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Pierce Streptavidin and Conjugates


Purified, conjugated and activated streptavidin for biotin-binding methods.

Pierce Streptavidin, Horseradish Peroxidase Conjugated

Thermo Scientific Pierce Streptavidin and Conjugates include streptavidin in a purified form, conjugated to enzymes or fluorophores or activated for crosslinking to sulfhydryl or carbonyl groups.

These purified and conjugated forms of recombinant streptavidin protein enable detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin and the streptavidin-HRP conjugates (peroxidase-conjugated streptavidin) are supplied lyophilized in phosphate-based buffers for immediate reconstitution with water. Alkaline Phosphatase conjugates are supplied as a liquid in a Tris-based buffer.

Highlights:

  • Recombinant streptavidin – high-quality protein, 53kDa, pI = 6.8 to 7.5
  • Pure protein and enzyme conjugates – choose pure, unconjugated protein for biotin blocking and controls; enzyme conjugates for substrate-based detection
  • Fluorophore conjugates – standard fluorescent forms (fluorescein, rhodamine, etc.) for traditional immunofluorescence techniques
  • Activated preparations – choose a maleimide-activated or hydrazide-activated form to prepare a custom streptavidin conjugate with sulfhydryl- or carbohydrate-containing molecule

Product Details:

Streptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Thermo Scientific Pierce Streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.

Applications for enzyme-labeled (HRP, AP) streptavidin:

  • Enzyme immunoassays (EIA), including enzyme-linked immunsorbent assays (ELISA) with biotinylated antibodies
  • Cell and tissue staining (for light microscopy)
  • Western blotting (WB) and other membrane-based biotin detection
Also see:

High Sensitivity
Streptavidin Conjugates


Much greater sensitivity and performance in many applications
   

Applications for fluorescent (FITC, TRITC or Texas Red* Fluor-labeled) streptavidin:

  • Immunofluorescent assays (IFA)
  • Tissue staining (for fluorescence microscopy)
  • Blot immunostaining
  • Cell staining (incl. fluorescent activated cell sorting)
Also see:

DyLight Fluor
Streptavidin Conjugates


Surpass the performance of traditional fluorescein and rhodamine fluors in many applications.

References:

  1. Chaiet, I. and Wolf, F.J. (1964). Arch. Biochem. Biophys. 106, 1-5.
  2. Savage, M.D., et al. (1992). Avidin-Biotin Chemistry: A Handbook. Rockford, Illinois: Pierce Chemical Company.
  3. Wilchek, M. and Bayer, E.A. (1983). Anal. Biochem. 71, 1-32.
  4. Zuk, P.A. and Elferink, L.A. (2000). J. Biol. Chem. 275, 26754-26764.
  5. Conti, L.R., et al. (2001). J. Biol. Chem. 276, 41270-41278.

Related Resources:

Immunohistochemistry (IHC) detection technical guide

Related Products:

Poly-HRP Streptavidin Conjugate
High Sensitivity Streptavidin HRP Conjugates
DyLight Fluor Streptavidin Conjugates
Streptavidin Agarose Resin
NeutrAvidin Protein and Conjugates


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
21122 Streptavidin
Formulation: Lyophilized powder from protein in buffered solution
Sufficient For: Binding 17 to 24µg biotin per mg protein
1mg MSDS for product #21122 Streptavidin Certificate of Analysis for product #21122 Streptavidin $119.00

Add to cart
21125 Streptavidin
Formulation: Lyophilized powder from protein in buffered solution
Sufficient For: Binding 17 to 24µg biotin per mg protein
5mg MSDS for product #21125 Streptavidin Certificate of Analysis for product #21125 Streptavidin $360.00

Add to cart
21125B Streptavidin
Formulation: Lyophilized powder from protein in buffered solution
Sufficient For: Binding 17 to 24µg biotin per mg protein
Custom MSDS for product #21125B Streptavidin Certificate of Analysis for product #21125B Streptavidin
21135 Streptavidin
Formulation: Lyophilized powder from protein in buffered solution
Sufficient For: Binding 17 to 24µg biotin per mg protein
100mg MSDS for product #21135 Streptavidin Certificate of Analysis for product #21135 Streptavidin $1,100.00

Add to cart
21145 Streptavidin
Formulation: Lyophilized powder from protein in buffered solution
Sufficient For: Binding 17 to 24µg biotin per mg protein
1000mg MSDS for product #21145 Streptavidin Certificate of Analysis for product #21145 Streptavidin $8,200.00

Add to cart
21126 Streptavidin, Horseradish Peroxidase Conjugated
Formulation: Lyophilized powder from protein in buffered saline solution

Also consider our High Sensitivity Streptavidin-HRP Conjugates
1mg MSDS for product #21126 Streptavidin, Horseradish Peroxidase Conjugated Certificate of Analysis for product #21126 Streptavidin, Horseradish Peroxidase Conjugated $164.00

Add to cart
21124 Streptavidin, Horseradish Peroxidase Conjugated
Formulation: Lyophilized powder from protein in buffered saline solution
2mg MSDS for product #21124 Streptavidin, Horseradish Peroxidase Conjugated Certificate of Analysis for product #21124 Streptavidin, Horseradish Peroxidase Conjugated $296.00

Add to cart
21127 Streptavidin, Horseradish Peroxidase Conjugated
Formulation: Lyophilized powder from protein in buffered saline solution
5mg MSDS for product #21127 Streptavidin, Horseradish Peroxidase Conjugated Certificate of Analysis for product #21127 Streptavidin, Horseradish Peroxidase Conjugated $513.00

Add to cart
21127B Streptavidin, Horseradish Peroxidase Conjugated
Formulation: Lyophilized powder from protein in buffered saline solution
Custom MSDS for product #21127B Streptavidin, Horseradish Peroxidase Conjugated Certificate of Analysis for product #21127B Streptavidin, Horseradish Peroxidase Conjugated
21324 Streptavidin, Alkaline Phosphatase Conjugated
Formulation: Approx. 2 to 4mg/mL in buffered saline solution and 50% glycerol
1mg MSDS for product #21324 Streptavidin, Alkaline Phosphatase Conjugated Certificate of Analysis for product #21324 Streptavidin, Alkaline Phosphatase Conjugated $200.00

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21323 Streptavidin, Alkaline Phosphatase Conjugated
Formulation: Approx. 2 to 4mg/mL in buffered saline solution and 50% glycerol
3mg MSDS for product #21323 Streptavidin, Alkaline Phosphatase Conjugated Certificate of Analysis for product #21323 Streptavidin, Alkaline Phosphatase Conjugated $451.00

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21224 Streptavidin, Fluorescein (FITC) Conjugated
Formulation: Lyophilized powder from protein in buffered saline solution

Also consider our complete line of DyLight Fluor Streptavidin Conjugates
1mg MSDS for product #21224 Streptavidin, Fluorescein (FITC) Conjugated Certificate of Analysis for product #21224 Streptavidin, Fluorescein (FITC) Conjugated $195.00

Add to cart
21724 Streptavidin, Rhodamine (TRITC) Conjugated
Formulation: Lyophilized powder from protein in buffered saline solution
1mg MSDS for product #21724 Streptavidin, Rhodamine (TRITC) Conjugated Certificate of Analysis for product #21724 Streptavidin, Rhodamine (TRITC) Conjugated $195.00

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21627 Streptavidin, R-Phycoerythrin Conjugated
Formulation: Lyophilized powder from approx. 0.5mg/mL protein in buffered saline solution containing bovine serum albumin
1mL MSDS for product #21627 Streptavidin, R-Phycoerythrin Conjugated Certificate of Analysis for product #21627 Streptavidin, R-Phycoerythrin Conjugated $175.00

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21629 Streptavidin, Allophycocyanin Conjugated
Formulation: Lyophilized powder from approx. 0.5mg/mL protein in buffered saline solution containing bovine serum albumin
0.5mL MSDS for product #21629 Streptavidin, Allophycocyanin Conjugated Certificate of Analysis for product #21629 Streptavidin, Allophycocyanin Conjugated $175.00

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21102 Maleimide-Activated Streptavidin
Formulation: Lyophilized powder from activated protein in buffered saline solution
Sufficient For: Conjugation to sulfhydryl groups of approx. 1mg of antibody (150kDa) or molar equivalent
1mg Product Instructions for product #21102 Maleimide-Activated Streptavidin MSDS for product #21102 Maleimide-Activated Streptavidin Certificate of Analysis for product #21102 Maleimide-Activated Streptavidin $161.00

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21120 Hydrazide-Activated Streptavidin
Formulation: Lyophilized powder from activated protein in buffered saline solution
Sufficient For: Conjugation to oxidized sugar groups of approx. 2mg of antibody (150kDa) or molar equivalent
2mg Product Instructions for product #21120 Hydrazide-Activated Streptavidin MSDS for product #21120 Hydrazide-Activated Streptavidin Certificate of Analysis for product #21120 Hydrazide-Activated Streptavidin $133.00

Add to cart
21120B Hydrazide-Activated Streptavidin
Formulation: Lyophilized powder from activated protein in buffered saline solution
Sufficient For: Conjugation to oxidized sugar groups of approx. 2mg of antibody (150kDa) or molar equivalent
Custom MSDS for product #21120B Hydrazide-Activated Streptavidin Certificate of Analysis for product #21120B Hydrazide-Activated Streptavidin

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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