Update profile
    Change password
    Sign out

Pierce Streptavidin Magnetic Beads


Superior biotin-binding particles for automated magnetic separations.

Pierce Streptavidin Magnetic Beads for Thermo Scientific Automated Solutions.

Thermo Scientific Pierce Streptavidin Magnetic Beads accelerate throughput for automated magnetic purification of biotinylated molecules.

These streptavidin magnetic particles are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher 96 and KingFisher Flex Instruments, but the beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand. The iron oxide, super-paramagnetic particles offer superior performance (high capacity and low nonspecific binding) compared with other commercial magnetic beads.

Highlights:

  • High-performance beads – non-aggregating, pre-blocked, iron oxide, superparamagnetic microparticles provide exceptional uniformity for automated HTS and manual applications alike
  • Stable immobilization chemistry – streptavidin is immobilized using leach-resistant chemistry
  • High capacity – superior quality beads with high binding capacity provide rapid and efficient biomolecule purification from complex samples
  • Low non-specific binding – stable, pre-blocked beads provide clean purification products (e.g., antigen eluted in IP with biotinylated antibody) that are compatible with mass spectrometry analysis and other downstream analyses
  • Superior performance – nearly three times higher binding capacity than typical beads from other suppliers, allowing the use of smaller amounts per experiment

Applications:

  • Immunoprecipitate antigens (using biotinylated antibodies) from a wide variety of sources
  • Co-immunoprecipitate interaction complexes using biotinylated antibodies
  • Capture protein-protein interactions in pull-down assays using biotinylated "bait" proteins
  • Isolate biotin-labeled DNA-protein complexes from cell or tissue extracts
  • Capture single-stranded biotinylated DNA oligos
  • Isolate biotinylated PCR products

Product Details:

Pierce Streptavidin Magnetic Beads use a recombinant form of streptavidin with a mass of 53kDa and a near-neutral isoelectric point (pI). The protein is a tetramer having four biotin-binding sites. Unlike avidin, streptavidin has no carbohydrate groups, resulting in low nonspecific binding. The high-affinity interaction between streptavidin and biotin cannot be dissociated efficiently except with very harsh conditions, such as boiling in sample loading buffer for SDS-PAGE or 8M guanidine•HCl, pH 1.5. Consequently, it is often possible to elute binding partners in an interaction complex without also eluting the biotinylated component.

Characteristics of Thermo Scientific Pierce Streptavidin Magnetic Beads.
Composition: Iron oxide particles covalently coated with a monolayer of recombinant streptavidin protein
Magnetization: Superparamagnetic (no magnetic memory)
Mean Diameter: 1µm
Density: 2g/cm3
Concentration: 10mg/mL
Binding Capacity: ~55µg biotinylated rabbit IgG per mg of beads;
~3500pmol biotinylated fluorescein per mg of beads
Note: These streptavidin magnetic bead solutions are NOT tested and certified to be RNase-free.

More efficient immunoprecipitation with Pierce Streptavidin Magnetic BeadsBetter immunoprecipitation results with Pierce Streptavidin Magnetic Beads. MOPC cell lysate (0.75mg per sample) was incubated overnight at 4°C with and without 10µg biotinylated Grp94 antibody. Pierce Streptavidin Magnetic Beads (Part No. 88817) and Dynabeads™ MyOne™ Streptavidin T1 Beads (Life Technologies) were added to a 96 deep-well plate (0.5mg or 0.25mg per well). Using the Thermo Scientific KingFisher 96 Instrument, the beads were washed with Tris-buffered saline containing 0.1% Tween-20, incubated 1 hour with the antigen sample/antibody mixture, washed three times and then eluted for 10 minutes at 96°C with SDS-PAGE reducing sample buffer. Eluates were resolved by SDS-PAGE and analyzed by Western Blot with anti-Grp94 antibody. About 0.25mg of Pierce Beads gave the same yield as 0.5mg of MyOne Beads.

Higher binding capacity with Pierce Streptavidin Magnetic Beads. Thermo Scientific Pierce Streptavidin Magnetic Beads and Dynabeads™ MyOne™ Streptavidin T1 Beads (Life Technologies) were added to a 96 deep-well plate (1mg beads per well). Using the Thermo Scientific KingFisher 96 Instrument, the beads were washed with phosphate-buffered saline containing 0.05% Tween-20. The beads were then incubated for 1 hour with varying amounts of biotinylated rabbit IgG (20 to 225µg). After binding, the beads were washed three times and eluted. Binding was calculated by subtracting the amount of protein in the flow-through from the amount of protein loaded using the Micro BCA Protein Assay (Part No. 23235). Higher binding capacity with Pierce Streptavidin magnetic beads

Related Resources:

Review of automated magnetic separations for proteomics (includes selection guide)

Related Products:

Other Pierce Magnetic Beads
Thermo Scientific KingFisher Magnetic Particle Processors


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
88816 Pierce Streptavidin Magnetic Beads
Formulation: Streptavidin on paramagnetic iron oxide particles (1µm dia.) slurried in water with sodium azide
Sufficient For: Binding approx. 55µg biotinylated rabbit IgG per mg of beads (approx. 3500 pmol biotinylated fluorescein per mg of beads)
1mL Product Instructions for product #88816 Pierce Streptavidin Magnetic Beads MSDS for product #88816 Pierce Streptavidin Magnetic Beads Certificate of Analysis for product #88816 Pierce Streptavidin Magnetic Beads $232.00

Add to cart
88817 Pierce Streptavidin Magnetic Beads
Formulation: Streptavidin on paramagnetic iron oxide particles (1µm dia.) slurried in water with sodium azide
Sufficient For: Binding approx. 55µg biotinylated rabbit IgG per mg of beads (approx. 3500 pmol biotinylated fluorescein per mg of beads)
5mL Product Instructions for product #88817 Pierce Streptavidin Magnetic Beads MSDS for product #88817 Pierce Streptavidin Magnetic Beads Certificate of Analysis for product #88817 Pierce Streptavidin Magnetic Beads $790.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Share This Page
 
 

Follow Us
  Email Sign-up  Email Announcements

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747