Thermo Scientific Pierce High Sensitivity Streptavidin HRP Conjugate is an exclusive peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.
This specially-manufactured variety of HRP-conjugated streptavidin protein meets the demands of today's scientists for more sensitive detection in ELISA and Western blotting applications. The conjugate is suitable for use with chemiluminescent, chemifluorescent or colorimetric substrates. Each High Sensitivity HRP conjugate is packaged in an easy-to-use stabilized solution that enables convenient storage at 4°C for at least one year.
- High sensitivity – detect low levels of target without background; obtain high signal-to-noise ratios
- Cost effective – use less conjugate in Western blotting and ELISA applications and still obtain excellent results
- Flexible – compatible with typical chemiluminescent, fluorescent and colorimetric peroxidase substrates
- Convenient – ready-to-use stabilized liquid format means there is no waiting for thawing and no need to aliquot
Our High Sensitivity HRP Conjugates outperform the competition on target detection, signal intensity and signal-to-noise ratios. Experiments indicate that the High Sensitivity Streptavidin-HRP can be diluted eight-to 10-fold more than traditional HRP conjugates and still produce similar results.
| Thermo Scientific High Sensitivity Streptavidin-HRP enables low-level target detection with high signal-to-noise ratios. Recombinant GST (Part No. 20237) was serially diluted (0-10,000pg/ml) with StartingBlock (PBS) Blocking Buffer (Part No. 37538). Each dilution (100µL) was added to a 96-well glutathione-coated plate (Part No. 15340) in four replicates, including a negative control. The plate was incubated for 60 minutes at room temperature and washed three times with PBS Tween-20 Detergent (Part No. 28348). Biotinylated anti-GST (100µL at 250ng/mL; Santa Cruz) was added to all wells. The plate was incubated for 30-60 minutes at room temperature and washed three times with wash buffer. Streptavidin-HRP conjugates were diluted in blocking buffer as per manufacturer's directions. High Sensitivity Streptavidin-HRP was diluted 1:10,000 and conjugates from other suppliers were diluted to 1:1,000. The conjugate solutions (100µL) were added to the plate and incubated for 60 minutes (light protected) at room temperature. The plate was washed five times with wash buffer and 150µL/well of SuperSignal ELISA Pico Substrate (Part No. 37070) was added. Signal intensity was measured using a luminometer.
Thermo Scientific High Sensitivity HRP Conjugates provide superior performance in Western blotting. Western blot of identical HeLa lysate samples probed with a biotinylated anti-GAPDH antibody (1µg/mL) and then with respective streptavidin-HRP conjugates. A dilution of 1:4000 was used for Thermo Scientific High Sensitivity Streptavidin-HRP, and a dilution of 1:1000 was used for conjugates from the other suppliers. After washing, Thermo Scientific Pierce ECL Substrate (Part No. 32106) was added using identical conditions. Membranes were exposed to film for 5 seconds.
Excellent IHC staining of GAPDH and cytokeratin 18 in human colon carcinoma with Thermo Scientific High Sensitivity HRP Conjugates. Formalin-fixed paraffin-embedded human colon carcinoma tissues were stained using Metal Enhanced DAB Substrate (Thermo Scientific Pierce Immunohistochemistry Peroxidase Detection Kit, Part No. 36000). The tissues were incubated with either biotinylated anti-GAPDH or rabbit anti-cytokeratin 18 followed by biotinylated anti-rabbit IgG (left panels) or blocking buffer only (right panels). The sections were subsequently incubated with Thermo Scientific High Sensitivity NeutrAvidin-HRP (top panels) or Streptavidin-HRP (bottom panels). Tissues were counterstained using the Harris-modified hematoxylin solution (blue staining in all panels). GAPDH and Cytokeratin 18, stained using the NeutrAvidin HRP and streptavidin HRP, appear brown (left panels) while the Negative Control panels shows no brown staining in the tissue.
Immunohistochemistry (IHC) detection technical guide
Poly-HRP Streptavidin Conjugate (including conjugate usage suggestions)
High Sensitivity NeutrAvidin HRP Conjugate
All Streptavidin, NeutrAvidin and Avidin Conjugates