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Streptavidin Coated Plates - High Sensitivity


Assay plates optimized to capture low concentrations of biotinylated molecules.

Pierce High Sensitivity Streptavidin and NeutrAvidin Plates

Thermo Scientific Pierce High Sensitivity Streptavidin Coated Plates are biotin-binding microplates that are coated to maximize capture efficiency of biotinylated probes and control background in assays for low levels of target analyte.

These Thermo Scientific Pierce Coated Plates are coated using proprietary surface-activation technology to achieve maximum efficient coating of active streptavidin biotin-binding protein. The high sensitivity plates enable detection of low concentrations (ng/mL) of biotinylated antibodies and other proteins. Whether to detect signal by fluorescent, colorimetric or chemiluminescent methods, these plates make it easy to develop an ELISA for any sample with a low-level target.

Highlights:

  • Sensitive – detect down to 5ng/mL of biotinylated IgG
  • Specific – low background with high signal-to-noise ratios
  • Versatile – use either fluorescence, chemiluminescence or colorimetric detection
  • Robust – broad dynamic range
  • Ready to use – plates are supplied pre-blocked to save time

Product Details:

Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. Thermo Scientific NeutrAvidin Protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.

Comparison of Streptavidin Coated Plate types. Detection Ranges were determined using Thermo Scientific QuantaBlu Fluorogenic Peroxidase Substrate.
  High Sensitivity
(HS)
High Binding
Capacity (HBC)
Standard Binding
Capacity (SBC)
Application Detect low concentrations of biotinylated molecules Detect high concentrations of biotinylated molecules General ELISA screening applications
Biotinylated Protein Minimum Size > 26kDa > 8kDa > 8kDa
Detection Range
Streptavidin Plates
5 to 300ng/mL
(current page)
62 to 10,000ng/mL
(visit page)
31 to 1250ng/mL
(visit page)
Detection Range
NeutrAvidin Plates
5 to 125ng/mL
(visit page)
15 to 2500ng/mL
(visit page)
15 to 300ng/mL
(visit page)

 

Biotin Binding Plates Detect low concentrations of biotinlyated target molecules using Thermo Scientific High Sensitivity Coated Plates. Biotinylated rabbit IgG was incubated for 30 minutes in streptavidin-coated high sensitivity plates (HS) or streptavidin-coated standard binding capacity (SBC) microplates. Plates were washed three times with Tris-buffered saline and incubated with goat anti-rabbit IgG conjugated to horseradish peroxidase (100ng/mL) for 30 minutes. Plates were washed and incubated with Thermo Scientific QuantaBlu Fluorogenic Peroxidase Substrate (Part No. 15169). The fluorescent signal was measured at 430nm.

Related Resources:

Avidin-biotin systems technical guide
Assay development and ELISA technical guide

Related Products:

Other Streptavidin and NeutrAvidin Coated Microplates
Blocking Buffers
ELISA Substrates

Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
15520 Streptavidin Coated High Sensitivity Plates, Clear, 8-Well Strip
Formulation: Clear polystyrene 96-well strip plates coated at 200μL/well with streptavidin and blocked at 250μL/well with Blocker BSA Buffer.
Sufficient For: Assaying samples containing an equivalent of 5 to 300ng/mL biotinylated IgG (33 to 2000pmol/mL protein)
5 plates Product Instructions for product #15520 Streptavidin Coated High Sensitivity Plates, Clear, 8-Well Strip MSDS for product #15520 Streptavidin Coated High Sensitivity Plates, Clear, 8-Well Strip Certificate of Analysis for product #15520 Streptavidin Coated High Sensitivity Plates, Clear, 8-Well Strip $207.00

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15525 Streptavidin Coated High Sensitivity Plates, Black, 8-Well Strip
Formulation: Black polystyrene 96-well strip plates coated at 200μL/well with streptavidin and blocked at 250μL/well with Blocker BSA Buffer.
Sufficient For: Assaying samples containing an equivalent of 5 to 300ng/mL biotinylated IgG (33 to 2000pmol/mL protein)
5 plates Product Instructions for product #15525 Streptavidin Coated High Sensitivity Plates, Black, 8-Well Strip MSDS for product #15525 Streptavidin Coated High Sensitivity Plates, Black, 8-Well Strip Certificate of Analysis for product #15525 Streptavidin Coated High Sensitivity Plates, Black, 8-Well Strip $207.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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