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Streptavidin Agarose and Columns


Standard-capacity affinity beads ideal for immunoprecipitation with biotinylated antibodies.

Streptavidin Agarose Resin

Thermo Scientific Pierce Streptavidin Agarose is a standard-capacity beaded agarose resin of immobilized recombinant streptavidin protein for use in a variety of biotin-binding affinity purification methods.

Pierce Streptavidin Agarose consists of purified recombinant streptavidin that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose. Streptavidin is a tetrameric biotin-binding protein. The agarose beads have physical and chemical properties that enable them to be used in a variety of batch- or column-type affinity procedures. Biotinylated antibodies, proteins, peptides, nucleic acids and other molecules or interaction complexes can be captured, immunoprecipitated, removed or purified from samples using this streptavidin resin.

Highlights:

  • Streptavidin – purified recombinant streptavidin protein (53kDa, near-neutral pI); tetrameric with four biotin-binding site per molecule
  • Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
  • Inert and stable – streptavidin is immobilized by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
  • Standard capacity – this variety of beads has a normal load of immobilized streptavidin, providing a binding capacity of 1 to 3mg of biotinylated BSA per mL of resin
  • Two formats – available in several bottle sizes of resin slurry and as pre-packed 5mL-capacity spin columns containing 1mL of resin

Applications:

  • Purification of membrane antigens in conjunction with biotinylated monoclonal antibodies
  • Isolation of cell-surface proteins that have been labeled with a cleavable biotinylation reagent
  • Isolation of cell-surface glycoproteins using biotinylated Concanavalin A
  • Recovery of single-stranded biotinylated DNA for dideoxy sequencing
  • Streptavidin-coated plates used for direct solid-phase sequencing
  • In vitro mutagenesis with streptavidin-coated plates
  • DNA fingerprinting with streptavidin-coated plates

Product Details:

Streptavidin is a tetrameric protein containing four biotin-binding sites similar to avidin. The native protein from Streptomyces avidinii is carbohydrate-free, has an acidic pI of 5.5 and a mass of 75 kDa. The recombinant protein used to manufacture Thermo Scientific Pierce Streptavidin Resins has a near-neutral isoelectric point and a mass of 53kDa. Streptavidin generally has less nonspecific binding than avidin (from chicken egg white) because of the absence of carbohydrates.

Comparison of available Thermo Scientific Pierce Streptavidin Resins. Binding-capacity specifications (Capacity) are expressed as milligrams of biotinylated BSA bound per milliliter of resin. Store all resins in water or buffer at 4°C; do not freeze. Also consider our NeutrAvidin Resins.
Product Support Capacity Available Formats
Streptavidin
Agarose Resin
Crosslinked 6% beaded agarose 1 to 3mg Bulk resin (2, 5, 10mL);
Spin columns (1mL resin)
High Capacity
Streptavidin
Agarose Resin
Crosslinked 6% beaded agarose >10mg Bulk resin (2, 5, 10mL);
Chromatography cartridges (1, 5mL)
Streptavidin
UltraLink Resin
Co-polymer of crosslinked bisacrylamide and azlactone >2mg Bulk resin (2, 5mL)
Streptavidin Plus UltraLink Resin Co-polymer of crosslinked bisacrylamide and azlactone >4mg Bulk resin (2, 5mL)

Properties of crosslinked 6% beaded agarose (CL-6B):

  • Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
  • Average Particle Size: 45 to 165 microns
  • Exclusion Limit: 10,000 to 4,000,000 daltons
  • Maximum Volumetric Flow Rate: approx. 1mL/minute (for 1cm diameter column)
  • Maximum Linear Velocity: 30cm per hour
  • Maximum Pressure: less than 25psi (1.5 bar)

References (all Streptavidin Resins):

  1. Nielsen K. H. et al. (2011) Synergistic activation of eIF4A by eIF4B and eIF4G. Nucleic Acids Res. 39, 2678-89.
  2. Havel L. S. et al. (2011) Preferential accumulation of N-terminal mutant huntingtin in the nuclei of striatal neurons is regulated by phosphorylation. Hum. Mol. Genet. 20, 1424-37.
  3. Caraballo J. C. et al. (2011) Hypoxia increases transepithelial electrical conductance and reduces occludin at the plasma membrane in alveolar epithelial cells via PKC-{zeta} and PP2A pathway. Am J Physiol Lung Cell Mol Physiol. 300, L569-78.
  4. Nielsen K. H. et al. (2009) Mechanism of ATP turnover inhibition in the EJC. RNA. 15, 67-75.
  5. Lamkanfi M. et al. (2008) Targeted peptidecentric proteomics reveals caspase-7 as a substrate of the caspase-1 inflammasomes. Mol. Cell. Proteomics. 7, 2350-63.

Related Products:

Other Biotin-binding Affinity Resins
Purified Streptavidin and Streptavidin Conjugates
Streptavidin Coated Plates
Pierce Streptavidin Magnetic Beads


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
20347 Streptavidin Agarose
Formulation: Streptavidin on crosslinked 6% beaded agarose, slurried in water with sodium azide
Sufficient For: Binding 15 to 28µg biotin per mL of resin (1 to 3mg biotinylated BSA per mL of resin)
2mL Product Instructions for product #20347 Streptavidin Agarose MSDS for product #20347 Streptavidin Agarose Certificate of Analysis for product #20347 Streptavidin Agarose $193.00

Add to cart
20349 Streptavidin Agarose
Formulation: Streptavidin on crosslinked 6% beaded agarose, slurried in water with sodium azide
Sufficient For: Binding 15 to 28µg biotin per mL of resin (1 to 3mg biotinylated BSA per mL of resin)
5mL Product Instructions for product #20349 Streptavidin Agarose MSDS for product #20349 Streptavidin Agarose Certificate of Analysis for product #20349 Streptavidin Agarose $339.00

Add to cart
20353 Streptavidin Agarose
Formulation: Streptavidin on crosslinked 6% beaded agarose, slurried in water with sodium azide
Sufficient For: Binding 15 to 28µg biotin per mL of resin (1 to 3mg biotinylated BSA per mL of resin)
10mL Product Instructions for product #20353 Streptavidin Agarose MSDS for product #20353 Streptavidin Agarose Certificate of Analysis for product #20353 Streptavidin Agarose $505.00

Add to cart
20351 Streptavidin Agarose Columns, 1mL
Formulation: 5mL-capacity centrifuge columns containing 1mL of resin
Sufficient For: Binding 15 to 28µg biotin per column (1 to 3mg biotinylated BSA per column)
5 columns Product Instructions for product #20351 Streptavidin Agarose Columns, 1mL MSDS for product #20351 Streptavidin Agarose Columns, 1mL Certificate of Analysis for product #20351 Streptavidin Agarose Columns, 1mL $344.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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