Update profile
    Change password
    Sign out

Our content has moved! Check out our protein biology page on lifetechnologies.com  Go now >

Stop Solution for TMB

A ready-to-use sulfuric acid solution for use with TMB substrates.

Stop Solution for TMB

Stop Solution is 0.16M sulfuric acid for use with the ELISA substrate 3,3’,5,5’ - tetramethylbenzidine (TMB). In the presence of horseradish peroxidase (HRP) enzyme conjugates, TMB and peroxide react to produce a blue byproduct having maximum absorbance at 605nm. The color intensity is proportional to the amount of HRP activity, which in turn is related to the levels of target analyte in an optimized ELISA procedure. Addition of sulfuric acid stop solution changes the color to yellow (absorbance maximum at 450nm), stabilizing the color development to enable accurate measurement of the intensity at 450nm using a spectrophotometer or plate reader.



  • Product Number: N600
  • Size: 55mL (sufficient for about five 96-well microplates)
  • Storage: Shipped at ambient temperature. Store at room temperature or 2-8°C.
  • Application: ELISA: Following TMB incubation, add an amount of stop solution equal to the amount of TMB substrate added to each well of the ELISA plate (typically 50 - 100mL per well). Use at full strength.

Related Products:

ELISA Blocking Buffer (Product # N502)
ELISA Wash Buffer (Product # N503)
TMB Substrate Solution (Product # N301)
Standard HRP-Streptavidin for ELISA (Product # N100)
Other TMB Substrates
Other types of ELISA Substrates

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
N600 Stop Solution 55mL MSDS for product #N600 Stop Solution $71.00

Now available on lifetechnologies.com

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

You may also need
28360 - 20X TBS Tween-20 Buffer

34028 - 1-Step Ultra TMB-ELISA Substrate Solution

46430 - Restore PLUS Western Blot Stripping Buffer


Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2015 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747