Update profile
    Change password
    Sign out

STAT6 In-Cell ELISA Kits


Microplate assays to measure total and phospho-STAT6 in whole cells.

Pierce STAT6 In-Cell ELISA Kits

Thermo Scientific Pierce STAT6 In-Cell ELISA Kits are colorimetric and fluorescent microplate assays optimized to measure total and phosphorylated signal transducers and activators of transciption 6 (phospho-STAT6) in whole cells.

These ELISA kits for whole cells include a mouse monoclonal anti-STAT6 antibody, rabbit monoclonal anti-phospho-(Tyr641) STAT6 antibody, detection reagents and all buffers required for either colorimetric or near infrared detection. The STAT6 In-Cell ELISA Kits enable simultaneous detection of both total and phospho-(Tyr641) STAT6.

Highlights:

  • Measures total and phosphorylated signal transducers and activators of transciption 6 (STAT6) in whole cells using a 96- or 384-well microplates
  • Two reliable detection formats available:
    • colorimetric detection kit for use with standard microplate readers
    • near infrared fluorescent detection kit for use with IR imaging system
  • Whole-cell detection requires no cell lysis, protein extraction or special sample preparation
  • Provides better data and higher throughput than quantitative Western blotting
  • Less costly (one well vs. one lane) than Western blotting

Product Details:

Dose-response curve showing decrease in total and phospho-MAPK3/1 in HeLa cells following U0126 treatment Stimulation with EGF causes a 3-fold increase in the level of STAT6 phosphorylation A431 cells. Relative total and phospho-STAT6 levels were measured in A431 cells using the STAT6 Colorimetric In-Cell ELISA Kit. Cells were serum-starved overnight and stimulated with 100ng/ml EFG for five minutes immediately prior to fixation.The level of STAT6 phosphorylation increased approximately 3-fold in response to EGF stimulation where as total STAT6 was not significantly affected. Note: Absolute absorbance values cannot be directly compared between phospho and total STAT6 experiments because they use antibodies with different sensitivities.

Graphs showing EGF induced phosphorylation of STAT6 and dose-dependent inhibition by PD168393
Treatment with EGF increases phosphorylation of STAT6 in A431 cells. Relative total and STAT6 levels were measured in A431 cells using the (A) STAT6 Near Infrared In-Cell ELISA Kit. Cells were serum-starved overnight and then treated with EGF for five minutes immediately prior to fixation. The EGF-induced phosphorylation of STAT6 is reduced in a dose-dependent manner when cell receive a two hour pre-treatment with the minute EGFR inhibitor, PD168393 (B, STAT6 Colorimetric In-Cell ELISA Kit). Note: Absolute absorbance values cannot be directly compared between phospho and total STAT6 experiments because they use antibodies with different sensitivities.

Overview of STAT6 and phosphorylation of STAT6:

STAT6 belongs to the STAT family of transcription factors which regulate many aspects of cell growth, survival and differentiation. Inactive STAT proteins are located in the cytosol until phosphorylated by receptor associated kinases. Upon phosphorylation, STAT proteins form homodimers or heterodimers with other STAT proteins and translocate into the cell nucleus. STAT6 is known interact with the transcriptional regulators EP300, CREB, NFκB and is integral in the anti-apoptotic effects of IL-4 through regulation of BCL2L1/BCL-X(L) expression. The STAT6 In-Cell ELISA Kit enables simultaneous detection of both total and phospho-(Tyr641) STAT6.

Related Resources:

Overview and Menu of Target-Specific In-Cell ELISA Kits
Dylight Fluor Spectral Properties (Ex/Em)

Related Products:

ELISA reagent reservoirs and sealing tape
Secondary Antibodies and Conjugates For ELISAs and Western Blotting
DyLight Fluor Conjugated Secondary Antibodies and Biotin Binding Proteins

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
62208 STAT6 Colorimetric In-Cell ELISA Kit
Formulation: Multi-component kit
Sufficient For: 96 microplate wells of overnight-cultured cells

Kit Contents:
Blocking Buffer, 50mL
20X Tris Buffered Saline, 50mL
Surfact-Amps 20 (10% Detergent), 10mL
Surfact-Amps X-100 (10% Detergent), 10mL
HRP Conjugate, 0.14mL
TMB Substrate, 58mL
TMB Stop Solution, 55mL
Janus Green Whole-Cell Stain, 50mL
Elution Buffer, 2 x 30mL
Thin Plate Seal Assembly, 8 each
Anti-Phospho STAT6 (Y641) Antibody
Anti-STAT6 Antibody
96-test kit Product Instructions for product #62208 STAT6 Colorimetric In-Cell ELISA Kit MSDS for product #62208 STAT6 Colorimetric In-Cell ELISA Kit $380.00

Add to cart
62213 STAT6 Near Infrared In-Cell ELISA Kit
Formulation: Multi-component kit
Sufficient For: 96 microplate wells of overnight-cultured cells

Kit Contents:
Blocking Buffer, 100mL
20X Tris Buffered Saline, 50mL
Surfact-Amps 20 (10% Detergent), 10mL
Surfact-Amps X-100 (10% Detergent), 10mL
DyLight 680 Goat Anti-Mouse IgG (H+L), 55µL
DyLight 800 Goat Anti-Rabbit IgG (H+L), 55µL
Thin Plate Seal Assembly, 8 each
Anti-Phospho STAT6 (Y641) Antibody
Anti-STAT6 Antibody
96-test kit Product Instructions for product #62213 STAT6 Near Infrared In-Cell ELISA Kit MSDS for product #62213 STAT6 Near Infrared In-Cell ELISA Kit $380.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Share This Page
 
 

Follow Us
  Email Sign-up  Email Announcements

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747