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StartingBlock Blocking Buffers


Simplify the selection of a blocker for Western blot and ELISA applications.

StartingBlock Blocking Buffers

Thermo Scientific StartingBlock Blocking Buffers are PBS and TBS formulations of a proprietary protein that provides exceptional performance and broad compatibility with membrane (Western blot) and 96-well plate (ELISA) assays.

Although no blocking buffer is ideal for every experimental situation, you are likely to obtain excellent results from the start with StartingBlock Blocking Buffers. This is because the blocking agent performs well with an extremely wide range of antibodies and assay systems. Our scientists have yet to encounter an antibody combination or other protein probing system in which StartingBlock Blocking Buffer is not compatible.

Highlights:

  • Compatible with many detection systems – Western blot, ELISA and IHC with antibody or avidin/biotin probes (blocker is serum and biotin-free)
  • Short blocking times – less than 15 minutes for nitrocellulose or PVDF membranes, almost instantaneous for polystyrene microplate wells
  • Strip and reprobe without reblocking – blots stay blocked even after stripping with Thermo Scientific Restore Stripping Buffer (Part No. 21059)
  • High signal:noise ratio – biotin- and serum protein-free formulation contributes to achieving signal-to-noise ratios from 10:1 to 20:1
  • Convenient formats – available ready-to-use 1X formulations in PBS and TBS, with and without Tween™ 20 detergent

Product Details:

Thermo Scientific StartingBlock Blocking Buffer performance after stripping and reprobing. Nitrocellulose and PVDF membranes that had been blocked with StartingBlock Blocking Buffer and used to detect a different primary target were then stripped with Thermo Scientific Restore Western Blot Stripping Buffer (Part No. 21059) and probed for the transferrin receptor (CD71). Detection was with SuperSignal West Dura Chemiluminescent Substrate (Part No. 34075) and exposed to film for 30 minutes and then again 24 hours. Very little background occurs with either membrane or exposure time, indicating exceptional blocking performance. StartingBlock Blocking Buffer performance after stripping and reprobing.

References:

  1. Alegria-Schaffer, A., et al. (2009). Performing and optimizing Western blots with an emphasis on chemiluminescent detection. Methods Enzymol. 463:573-99.

Related Resources:

Blocking buffers for Western blot and ELISA -- technical guide
Blocking strategies for immunohistochemistry -- technical guide

Related Products:

All Blocking Buffers


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
37538 StartingBlock (PBS) Blocking Buffer
Formulation: Proprietary protein formulation in phosphate-buffered saline, pH 7.5
Sufficient For: 30 blots or microplates at 30mL each
1L Product Instructions for product #37538 StartingBlock (PBS) Blocking Buffer MSDS for product #37538 StartingBlock (PBS) Blocking Buffer Certificate of Analysis for product #37538 StartingBlock (PBS) Blocking Buffer $165.00

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37578 StartingBlock (PBS) Blocking Buffer
Formulation: Proprietary protein formulation in phosphate-buffered saline, pH 7.5
Sufficient For: 3 blots or microplates at 30mL each
100mL Product Instructions for product #37578 StartingBlock (PBS) Blocking Buffer MSDS for product #37578 StartingBlock (PBS) Blocking Buffer Certificate of Analysis for product #37578 StartingBlock (PBS) Blocking Buffer $36.00

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37539 StartingBlock T20 (PBS) Blocking Buffer
Formulation: Proprietary protein formulation in phosphate-buffered saline, pH 7.5, with 0.05% Tween-20
Sufficient For: 30 blots or microplates at 30mL each
1L Product Instructions for product #37539 StartingBlock T20 (PBS) Blocking Buffer MSDS for product #37539 StartingBlock T20 (PBS) Blocking Buffer Certificate of Analysis for product #37539 StartingBlock T20 (PBS) Blocking Buffer $176.00

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37542 StartingBlock (TBS) Blocking Buffer
Formulation: Proprietary protein formulation in Tris-buffered saline, pH 7.5
Sufficient For: 30 blots or microplates at 30mL each
1L Product Instructions for product #37542 StartingBlock (TBS) Blocking Buffer MSDS for product #37542 StartingBlock (TBS) Blocking Buffer Certificate of Analysis for product #37542 StartingBlock (TBS) Blocking Buffer $165.00

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37579 StartingBlock (TBS) Blocking Buffer
Formulation: Proprietary protein formulation in Tris-buffered saline, pH 7.5
Sufficient For: 3 blots or microplates at 30mL each
100mL Product Instructions for product #37579 StartingBlock (TBS) Blocking Buffer MSDS for product #37579 StartingBlock (TBS) Blocking Buffer Certificate of Analysis for product #37579 StartingBlock (TBS) Blocking Buffer $36.00

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37543 StartingBlock T20 (TBS) Blocking Buffer
Formulation: Proprietary protein formulation in Tris-buffered saline, pH 7.5, with 0.05% Tween-20
Sufficient For: 30 blots or microplates at 30mL each
1L Product Instructions for product #37543 StartingBlock T20 (TBS) Blocking Buffer MSDS for product #37543 StartingBlock T20 (TBS) Blocking Buffer Certificate of Analysis for product #37543 StartingBlock T20 (TBS) Blocking Buffer $176.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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