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Spectra Multicolor Broad Range Protein Ladder


4-color MW marker especially for proteins between 10 and 260kDa.

Spectra Multicolor Broad Range Protein Ladder (Molecular Weight Marker)

Thermo Scientific Spectra Multicolor Broad Range Protein Ladder is a 4-color protein standard containing 10 prestained proteins (10 to 260kDa) for use in gel electrophoresis and Western blotting.

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Four different chromophores (blue, orange, green, pink) are bound to the different component proteins, producing a brightly colored ladder with an easy-to-remember pattern.

Highlights:

  • Size range – 10 proteins spanning 10 to 260kDa
  • Multicolor – four different colors for unambiguous band-size assignment
  • Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil
  • Sharp bands – color-coded bands of similar intensity for easy visualization
  • Quality tested – each lot evaluated by SDS-PAGE and Western blotting
  • Membrane-compatible – colored bands transfer to membranes for Western blotting

Includes:

  • Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.

Applications:

  • Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
  • Monitoring protein transfer onto membranes after Western blotting
  • Sizing of proteins on SDS-polyacrylamide gels and Western blots

Product Details:

SDS-PAGE band profile of the Thermo Scientific Spectra Multicolor Broad Range Protein Ladder.
SDS-PAGE band profile of the Thermo Scientific Spectra Multicolor Broad Range Protein Ladder. Images are from a 4-20% Tris-glycine gel (SDS-PAGE) and subsequent transfer to membrane.

 

Migration patterns of Spectra Multicolor Broad Range Protein Ladder.
Migration patterns of Spectra Multicolor Broad Range Protein Ladder in different electrophoretic conditions. The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.

References:

  1. Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680-685.
  2. Burnette, W.N. (1981). Western blotting: electrophoretic transfer of proteins from sodium dodecyl sulfate – polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A, Anal. Biochem., 112 (2), 195-203.
  3. Towbin, H., et al. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications, Proc. Natl. Acad. Sci. USA, 76, 4350-4354.

Related Resources:

Protein Molecular Weight Markers – product comparison and selection guide
Protein Gel Stains and Kits – compare colorimetric and fluorescent gel stains

Related Products:

Precise Protein Gels – browse all pre-cast gels for SDS-PAGE
BupH Dry Blend Electrophoresis Buffers – Tris-HEPES-SDS and other pouched electrophoresis buffers
Reducing Agents – 2-mercaptoethanol, DTT, TCEP and other reducing agents
Western blotting substrates and reagents – find kits, stripping buffers, blotting membranes and film


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
26623 Spectra Multicolor Broad Range Protein Ladder
Formulation: 10 proteins from 10 to 260kDa in reducing sample buffer
Sufficient For: 125 to 250 mini gel lanes at 10 to 20µL per lane
10 x 250µL Product Instructions for product #26623 Spectra Multicolor Broad Range Protein Ladder MSDS for product #26623 Spectra Multicolor Broad Range Protein Ladder Certificate of Analysis for product #26623 Spectra Multicolor Broad Range Protein Ladder $505.00

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26634 Spectra Multicolor Broad Range Protein Ladder
Formulation: 10 proteins from 10 to 260kDa in reducing sample buffer
Sufficient For: 25 to 50 mini gel lanes at 10 to 20µL per lane
2 x 250µL Product Instructions for product #26634 Spectra Multicolor Broad Range Protein Ladder MSDS for product #26634 Spectra Multicolor Broad Range Protein Ladder Certificate of Analysis for product #26634 Spectra Multicolor Broad Range Protein Ladder $115.00

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26634PROMO Spectra Multicolor Broad Range Protein Ladder
Formulation: 3 x Part No. 26634

Promotional item; expires Dec. 31, 2014
6 x 250µL $230.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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