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Sodium Deoxycholate

Disrupts and dissociates protein interactions.

Sodium Deoxycholate

Thermo Scientific Sodium Deoxycholate is an ionic detergent that is especially useful for disrupting and dissociating protein interactions.

Sodium deoxycholate (deoxycholic acid) is a water-soluble, bile-acid, ionic detergent commonly used in protein methods. It is most frequently used as a component of cell lysis buffers (e.g., RIPA buffer), but also has been used for liposome preparation, isolation of membrane proteins and lipids, preventing nonspecific binding in affinity chromatography and a cell culture media supplement.


  • Popular anionic, bile-acid detergent for many laboratory uses
  • Effective in disrupting and dissociating many types of protein interaction
  • Can be removed from solution by dialysis
  • Useful for elution or regeneration of certain kinds of affinity columns
  • High-purity compound with low UV absorptivity

Product Details:

The effectiveness of a detergent in any application depend upon the detergent's concentration. Too much or too little detergent can often have a deleterious effect. It is recommended that you examine a variety of detergent concentrations in your application. At concentrations above 2mM, cholate will form micelles having MW ~2000. The small micelle size allows easy removal by dialysis or gel filtration when needed. Note: removal of a detergent from a protein solution my result in protein precipitation and/or aggregation.

Sodium deoxycholate is the detergent recommended for stripping endotoxin (Lipopolysaccharide or LPS) from immobilized Polymyxin B columns. This is the recommended product for use with the Thermo Scientific Detoxi-Gel Endotoxin Removing Gel.

Chemical structure of sodium deoxycholic acid detergent
Chemical structure of sodium deoxycholate detergent.

Properties of Sodium deoxycholic acid:

  • Alternative Names: Sodium deoxycholic acid; deoxycholate, sodium salt
  • Chemical Name: 3, 12-α-Dihydroxy-5β-cholan-24-oic acid, monosodium salt
  • Molecular Weight: 414.6g
  • Detergent Class: Ionic (anionic)
  • Aggregation Number: 5 (average)
  • Micelle Molecular Weight: 2000g (average)
  • Critical Micelle Concentration (CMC): 2 to 6mM (0.083 to 0.249%, w/v)
  • Cloud Point: Unknown
  • Dialyzable: Yes

Specifications for Sodium Deoxycholate (Part No. 89904, 89905):

  • Formula: C24H39O4Na
  • Molecular Weight: 414.6g
  • Purity (by HPLC): ≥98%
  • Absorbance (1% Detergent Solution): 340nm <0.02; 280nm <0.04; 260nm <0.06
  • pH (1% Solution): 5 to 9
  • Solubility (in water at 20°C): ≥5%

Related Resources:

Review of detergents for cell lysis

Related Products:

Detergent Sampler Pack
Other Detergents for Protein Methods
Detergent Removal Resins

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
89904 Sodium Deoxycholate
Formulation: Sodium deoxycholic acid
5g MSDS for product #89904 Sodium Deoxycholate Certificate of Analysis for product #89904 Sodium Deoxycholate $94.00

Now available on lifetechnologies.com
89905 Sodium Deoxycholate
Formulation: Sodium deoxycholic acid
25g MSDS for product #89905 Sodium Deoxycholate Certificate of Analysis for product #89905 Sodium Deoxycholate $354.00

Now available on lifetechnologies.com

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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