Thermo Scientific SnakeSkin Dialysis Tubing, 3.5K MWCO, is an easy- and ready-to-use form of traditional dialysis membrane tubing that allows desalting and buffer exchange for 10 to 100mL samples.
SnakeSkin Dialysis Tubing, 3.5K MWCO, is composed of regenerated-cellulose dialysis tubing and supplied as an open, pleated (telescoped) tube. SnakeSkin Dialysis Tubing is supplied in eight inch (20cm) sticks containing 35 feet of dialysis tubing having a 16, 22, or 35mm circular internal diameter (I.D.). With similar capacity to 10.5 meters of 23 to 50 diameter dry flat width tubing, the hydrated SnakeSkin Dialysis Tubing holds ~2 to 10mL of sample per centimeter of length.
- Convenient – ready to use, pre-wetted, pleated tube
- High recovery – greater than 90% protein recovery
- Speed – dialysis is generally completed in 4 to 6 hours
- Stability – compatible with a variety of laboratory solutions, including acids, bases, hydrophobic solvents and alcohols
3.5K MWCO Tubing Specifications:
- Membrane Type: Regenerated cellulose
- Tubing Nominal Dry Thickness: 0.9 to 1.2mil (22 to 30µm)
- Glycerol Content: Trace
- Sulfur Content: 0.1% to 0.15%
- Heavy Metals Content: Trace
SnakeSkin Dialysis Tubing dimensions and capacities. Calculated volume per centimeter of tubing excludes membrane length used for tube closure. Some minor differences in volume may be observed due to membrane swelling and expansion.
Volume per cm
SnakeSkin Dialysis Tubing is available in three molecular weight cut offs: 3.5K, 7K and 10K and an internal diameter of 22mm. The 3.5K and 10K MWCO membranes are also available with internal diameters of 16 and 35mm. To prevent drying, store the product in its original packaging either at room temperature or refrigerated. Properly stored membrane is stable for at least one year.
The easy-open, pleated format of SnakeSkin Dialysis Tubing was developed to simplify large volume sample dialysis. To use, simply pull out and cut off the required length of tubing, fold over one end of tubing and apply a dialysis clip to close it, add sample at the open end and use a second clip to close the remaining end. Clips are sold separately. In comparison, many traditional dialysis tubing membranes that are supplied as dry, flattened rolls that are difficult to open and often require extensive soaking before they can be used.
The SnakeSkin membrane-pleating process does not change the tubing’s MWCO. Also, any low MW contaminants present are removed during the dialysis process. Because SnakeSkin Dialysis Tubing is made from the same type of regenerated cellulose as flat tubing, its dialysis performance matches that of conventional tubing.
Review of dialysis methods for protein research
Clips and other varieties of SnakeSkin Dialysis Tubing
Dialysis Flasks (250mL capacity)
Dialysis Concentrating Solution
Buffers for Protein Methods
All types and sizes of buffer-exchange devices