Update profile
    Change password
    Sign out

Our content has moved! Check out our protein biology page on lifetechnologies.com  Go now >


A long-chain disulfide crosslinker for strong yet reversible amine-to-sulfhydryl conjugation.

Pierce SMPT

Thermo Scientific Pierce SMPT is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form strong (hindered) yet cleavable (reducible) disulfide bonds with cysteine sulfhydryls.


  • Reactive groups: NHS ester and pyridyldithiol
  • Reactive towards: amino and sulfhydryl groups
  • Cleavable with disulfide reducing agents
  • Water-insoluble (dissolve first in DMF or DMSO)
  • Contains a hindered disulfide bond; has formed immunotoxins with improved stability
  • In vitro, an SMPT conjugate was as effective as conjugates formed with SPDP and 2-IT
  • Compare to other varieties of SPDP-type reagents, including pegylated forms

Product Details:

 4-Succinimidyloxycarbonyl-alpha-methyl-alpha(2-pyridyldithio)toluene; SMPT
Chemical structure of SMPT crosslinking reagent.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)
Other SPDP-type Reagents

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
21558 SMPT
Formulation: 4-Succinimidyloxycarbonyl-alpha-methyl-alpha(2-pyridyldithio)toluene; powder solid
Sufficient For: 129mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #21558 SMPT MSDS for product #21558 SMPT Certificate of Analysis for product #21558 SMPT $246.00

Now available on lifetechnologies.com

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

You may also need
34095 - SuperSignal West Femto Maximum Sensitivity Substrate

66430 - Slide-A-Lyzer Cassette Float Buoys (for 0.5mL to 3mL cassettes)

34028 - 1-Step Ultra TMB-ELISA Substrate Solution

23224 - BCA Protein Assay Reagent B


Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2015 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747