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An amine-to-sulfhydryl crosslinker with a long spacer arm (14.2 angstroms).

Pierce SMPH

Thermo Scientific Pierce SMPH is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long spacer arm (14.2 angstroms).


  • Reactive groups: NHS ester and maleimide
  • Reactive towards: amino and sulfhydryl groups
  • Slightly longer and more soluble than SMPB
  • NHS ester reacts with primary amines at pH 7-9, forming a stable amide bond
  • Maleimide reacts with -SH groups at pH 6.5-7.5, forming a stable thioether bond
  • Non-cleavable
  • Water-insoluble (dissolve first in DMF or DMSO)

Product Details:

Succinimidyl 6-[(beta-maleimidopropionamido)hexanoate]; SMPH
Chemical structure of SMPH crosslinking reagent.


  1. Rector, E.S., et al. (1978). A method for the preparation of protein-protein conjugates of predetermined composition. J. Immuno. Methods 24, 321-336.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
22363 SMPH
Formulation: Succinimidyl 6-[(beta-maleimidopropionamido)hexanoate]; solid
Sufficient For: 132mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22363 SMPH MSDS for product #22363 SMPH Certificate of Analysis for product #22363 SMPH $170.00

Now available on lifetechnologies.com
22363B SMPH
Formulation: Succinimidyl 6-[(beta-maleimidopropionamido)hexanoate]; solid
Custom MSDS for product #22363B SMPH Certificate of Analysis for product #22363B SMPH Bulk Quote Request

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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