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Slide-A-Lyzer MINI Dialysis Devices, 7K MWCO


The best system for small-volume protein dialysis at 7K MWCO.

Slide-A-Lyzer MINI Dialysis Unit

Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices, 7K MWCO are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules >7kDa in volumes from 10 to 100µL.

Slide-A-Lyzer MINI Dialysis Devices allow easy sample addition and removal using a standard laboratory pipette and can be used for single or arrays of samples. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.

Highlights:

  • Flexible format - enables user to dialyze a single sample (tube) or multiple samples (25 array)
  • Excellent sample recoveries - low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
  • One-step protocol - does not require laborious procedures or expensive equipment
  • 100% leak-tested - innovative design does not permit "wicking" that can occur in homemade devices
  • Time savings - performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation
Video of Slide-A-Lyzer MINI Devices in action!
 

Product Details:

Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialyzing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.

The 0.1mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5mL microcentrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1mL MINI Device, 100µL of pH 2.8 glycine buffer can be equilibrated with 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.

Slide-A-Lyzer MINI Dialysis Device, 7K MWCO Slide-A-Lyzer MINI Dialysis Device, 7K MWCO Slide-A-Lyzer MINI Dialysis Device, 7K MWCO

1. Pipette sample into device.

2. Cap and float in buffer.

3. Recover sample.

Sample dialysis with 0.1mL Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices. The 0.1mL MINI Devices are designed for 10 to 100µL samples. Dialysis can be performed against a beaker of solution (as shown here) or by placing an individual device in a microcentrifuge tube that is filled with dialysis buffer.

As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.

More Product Data

Related Resources:

Slide-A-Lyzer MINI Dialysis Device FAQ's
Slide-A-Lyzer Dialysis MINI Device, 0.1mL dimensions
Review of dialysis methods for protein research (and Technical Handbook)

Related Products:

Other MINI Dialysis Devices (2K to 20K MWCO)
Dialysis Cassettes, Tubing and Flasks (capacities up to 250mL)
Buffers for Protein Methods
All types and sizes of buffer-exchange devices


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
69560 Slide-A-Lyzer MINI Dialysis Device, 7K MWCO, 0.1mL
Formulation: Cellulose membrane in polypropylene tube with cap
Sufficient For: 50 samples, each 10 to 100µL
50 devices Product Instructions for product #69560 Slide-A-Lyzer MINI Dialysis Device, 7K MWCO, 0.1mL MSDS for product #69560 Slide-A-Lyzer MINI Dialysis Device, 7K MWCO, 0.1mL Certificate of Analysis for product #69560 Slide-A-Lyzer MINI Dialysis Device, 7K MWCO, 0.1mL $171.00

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69562 Slide-A-Lyzer MINI Dialysis Device, 7K MWCO, 0.1mL
Formulation: Cellulose membrane in polypropylene tube with cap
Sufficient For: 250 samples, each 10 to 100µL
250 devices Product Instructions for product #69562 Slide-A-Lyzer MINI Dialysis Device, 7K MWCO, 0.1mL MSDS for product #69562 Slide-A-Lyzer MINI Dialysis Device, 7K MWCO, 0.1mL Certificate of Analysis for product #69562 Slide-A-Lyzer MINI Dialysis Device, 7K MWCO, 0.1mL $523.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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