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Slide-A-Lyzer MINI Dialysis Devices, 3.5K MWCO


The best system for small-volume protein dialysis at 3.5K MWCO.

Slide-A-Lyzer MINI Dialysis Unit

Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices, 3.5K MWCO are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules >3.5kDa in volumes from 10µL to 2mL.

These 3.5K MWCO dialysis devices are available in three sizes (0.1, 0.5 and 2mL capacities), and are designed to allow easy sample addition and removal using a standard laboratory pipette. The MINI Devices are effective and convenient for accomplishing typical sample desalting or buffer exchange in 4 to 8 hours. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.

Highlights:

  • Three convenient sizes – dialysis devices for 0.1, 0.5 and 2mL samples fit standard 1.5mL-microcentrifuge, 15mL- and 50mL-conical tubes, respectively
  • Excellent sample recoveries – low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
  • One-step protocol – pipette sample into the MINI Device and place in tube containing the dialysis buffer; no laborious assembly, device preparation or expensive equipment are required
  • 100% leak-tested – innovative design does not permit "wicking" that can occur in homemade devices
  • Time savings – performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation
Video of Slide-A-Lyzer MINI Devices in action!

Product Details:

Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialyzing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.

The 0.5mL and 2mL MINI Dialysis Devices fit in and are capped by inserting them into the included 15mL and 50mL conical tubes, respectively. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimizes the risk of spills or contamination, and eliminates the need for floats or large beakers of buffer. Using these MINI Dialysis Devices, low molecular weight contaminant removal, buffer exchange and desalting, can be accomplished within 4 to 8 hours with efficiencies, rates, and recoveries very similar to conventional dialysis using a large dialysate volume (view data).

Protocol summary for Slide-A-Lyzer MINI Dialysis Devices
Sample dialysis with 0.5mL or 2mL Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices. Dialysis with the 2mL device is performed with a 50mL conical tube, as shown here. The 0.5mL device is designed for use with a 15mL conical tube.

The 0.1mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5mL microcentrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1mL MINI Device, 100µL of pH 2.8 glycine buffer can be equilibrated to 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.

Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO

1. Pipette sample into device.

2. Cap and float in buffer.

3. Recover sample.

Sample dialysis with 0.1mL Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices. The 0.1mL MINI Devices are designed for 10 to 100µL samples. Dialysis can be performed against a beaker of solution (as shown here) or by placing an individual device in a microcentrifuge tube that is filled with dialysis buffer.

As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.

More Product Data

Related Resources:

Slide-A-Lyzer MINI Dialysis Device FAQ's
Slide-A-Lyzer Dialysis MINI Device, 0.1mL dimensions
Review of dialysis methods for protein research (and Technical Handbook)

Related Products:

Other MINI Dialysis Devices (2K to 20K MWCO)
Dialysis Cassettes, Tubing and Flasks (capacities up to 250mL)
Buffers for Protein Methods
All types and sizes of buffer-exchange devices


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
69550 Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 0.1mL
Formulation: Cellulose membrane in polypropylene tube with cap
Sufficient For: 50 samples, each 10 to 100µL
50 devices Product Instructions for product #69550 Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 0.1mL MSDS for product #69550 Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 0.1mL Certificate of Analysis for product #69550 Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 0.1mL $171.00

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69552 Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 0.1mL
Formulation: Cellulose membrane in polypropylene tube with cap
Sufficient For: 250 samples, each 10 to 100µL
250 devices Product Instructions for product #69552 Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 0.1mL MSDS for product #69552 Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 0.1mL Certificate of Analysis for product #69552 Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 0.1mL $523.00

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69558 Slide-A-Lyzer MINI Dialysis Device Kit, 3.5K MWCO, 0.1mL
Formulation: Cellulose membrane in polypropylene tube with cap
Sufficient For: 10 samples, each 10 to 100µL


Kit Contents:
Slide-A-Lyzer MINI Dialysis Device, 10 devices
Slide-A-Lyzer MINI Dialysis Device Float Buoy, 1 float
10-device kit Product Instructions for product #69558 Slide-A-Lyzer MINI Dialysis Device Kit, 3.5K MWCO, 0.1mL MSDS for product #69558 Slide-A-Lyzer MINI Dialysis Device Kit, 3.5K MWCO, 0.1mL Certificate of Analysis for product #69558 Slide-A-Lyzer MINI Dialysis Device Kit, 3.5K MWCO, 0.1mL $75.00

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88400 Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 0.5mL
Formulation: Cellulose membrane in polypropylene insert enclosed in a 15mL conical tube with cap
Sufficient For: 25 samples, each 50 to 500µL
25 devices Product Instructions for product #88400 Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 0.5mL MSDS for product #88400 Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 0.5mL Certificate of Analysis for product #88400 Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 0.5mL $199.00

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88403 Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 2mL
Formulation: Cellulose membrane in polypropylene insert enclosed in a 50mL conical tube with cap
Sufficient For: 25 samples, each 200 to 2000µL
25 devices Product Instructions for product #88403 Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 2mL MSDS for product #88403 Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 2mL Certificate of Analysis for product #88403 Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 2mL $215.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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