Image of Slide-A-Lyzer Dialysis Cassettes and Flasks

Secure sample loading and retrieval without leaks

Slide-A-Lyzer dialysis cassettes, flasks, and MINI devices facilitate the rapid and trouble-free dialysis of sample volumes from 10 ul–250 mL. These devices enable easy sample loading, retrieval, and security for your dialysis experiments. Unlike standard flat tubing, Slide-A-Lyzer dialysis devices do not require knots or clips that can lead to leakage and sample loss.

The Slide-A-Lyzer G3 dialysis cassettes and flasks consist of rigid, free-standing frames that contain large surface area regenerated cellulose membranes, and a locking cap. Slide-A-Lyzer MINI devices have a unique cup-like design that can be placed in a foam float or inserted in conical tubes for easy and self-contained dialysis. Use Slide-A-Lyzer dialysis devices in various applications, including low molecular-weight contaminant and salt removal, buffer exchange, desalting, equilibrium dialysis, microconjugation reactions, and sample concentration.

Which dialysis device is best for your experiment?

 G3 CassettesMini Dialysis DeviceFlasksOriginal Cassettes
Volume range1–125 mL10 μL–2 mL150–250 mL100 μL–30 mL
External float requiredNoYes (0.1 mL size only)YesYes
Sample removalPipettePipettePipette, directly pouringSyringe
Molecular weight cutoffs (MWCOs)2K, 3.5K, 10K, 20K2K (0.1 mL size only), 3.5K, 10K, 20K2K, 3.5K, 10K, 20K2K, 3.5K, 7K, 10K, 20K

Choose your Slide-A-Lyzer device by volume and MWCO

Slide-A-Lyzer dialysis cassettes of various sizes

Slide-A-Lyzer G3 dialysis cassettes accommodate volumes ranging from 1–125 mL, and are available in 2K, 3.5K, 10K, and 20K molecular weight cutoffs (MWCOs).

Use Slide-A-Lyzer G3 dialysis cassettes in various applications, including low molecular-weight contaminant and salt removal, buffer exchange, and sample concentration.

Learn more about Slide-A-Lyzer dialysis cassettes

Choose your G3 dialysis cassette based on MWCO

Slide-A-Lyzer MINI dialysis devices have a unique cup-like design that is available in 3 volume sizes: 0.1 mL devices, which can be placed into a foam float during dialysis, and 0.5 mL and 2 mL sizes, which are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes serve as dialysis reservoirs for easy and self-contained dialysis and are available in 2K, 3.5K, 7K, 10K, and 20K MWCOs.

Use the Slide-A-Lyzer MINI Dialysis Devices in applications including low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, microconjugation reactions, and sample concentration.

Learn more about Slide-A-Lyzer MINI dialysis devices

Choose your MINI dialysis device based on MWCO

Slide-A-Lyzer dialysis flask

Slide-A-Lyzer dialysis flasks facilitate simple and effective dialysis in larger sample volumes ranging from 150 mL up to 250 mL. These devices accomplish typical buffer exchange in 8–24 hours and are available in 2K, 3.5K, 10K, and 20K MWCOs.

Use Slide-A-Lyzer dialysis flasks in various applications, including low molecular-weight contaminant and salt removal, buffer exchange, and sample concentration.

Learn more about Slide-A-Lyzer flasks

Choose your dialysis flask

Slide-A-Lyzer dialysis cassettes of various sizes

The original Slide-A-Lyzer dialysis cassettes facilitate the rapid and effective dialysis of sample volumes ranging from 100 μL to 30 mL in 2K, 3.5K, 7K, 10K, 20K MWCOs.

Use the original Slide-A-Lyzer dialysis cassettes for applications including low-molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration.

Choose your Slide-A-Lyzer dialysis cassette based on MWCO

Learn more about Slide-A-Lyzer cassettes

Table 1. Quantitative sample recovery.

Three sample volume batches of water (0.5 mL, 1.7 mL, and 3.0 mL) were loaded and recovered per the respective manufacturer’s instructions in a Slide-A-Lyzer Dialysis Cassette or conventional dialysis tubing to determine the volumes of recovery. Water volume recovered was determined gravimetrically.

Sample volume loadedSlide-A-Lyzer dialysis cassette % volume recoveryTraditional dialysis tubing % volume recovery
3.0 mL9.4792.32
1.7 mL99.3093.12
0.5 mL98.7687.51
PB2260_slide-a-lyzer_cassette_procedure1

1. Insert syringe needle through the gasket via one of the corner ports. Inject the sample, withdraw the excess air, and remove the syringe.

PB2260_slide-a-lyzer_cassette_procedure2

2. Attach a float buoy and dialyze. (Buoys also serve as convenient bench-top stands for the cassettes).

PB2260_slide-a-lyzer_cassette_procedure3

3. Insert empty syringe needle at a second corner port. Inject air to expand the cassette chamber, and then withdraw the dialyzed sample.

Figure 1. Slide-A-Lyzer dialysis cassette workflow

Slide-A-Lyzer dialysis cassettes of various sizes

Slide-A-Lyzer G3 dialysis cassettes accommodate volumes ranging from 1–125 mL, and are available in 2K, 3.5K, 10K, and 20K molecular weight cutoffs (MWCOs).

Use Slide-A-Lyzer G3 dialysis cassettes in various applications, including low molecular-weight contaminant and salt removal, buffer exchange, and sample concentration.

Learn more about Slide-A-Lyzer dialysis cassettes

Choose your G3 dialysis cassette based on MWCO

Slide-A-Lyzer MINI dialysis devices have a unique cup-like design that is available in 3 volume sizes: 0.1 mL devices, which can be placed into a foam float during dialysis, and 0.5 mL and 2 mL sizes, which are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes serve as dialysis reservoirs for easy and self-contained dialysis and are available in 2K, 3.5K, 7K, 10K, and 20K MWCOs.

Use the Slide-A-Lyzer MINI Dialysis Devices in applications including low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, microconjugation reactions, and sample concentration.

Learn more about Slide-A-Lyzer MINI dialysis devices

Choose your MINI dialysis device based on MWCO

Slide-A-Lyzer dialysis flask

Slide-A-Lyzer dialysis flasks facilitate simple and effective dialysis in larger sample volumes ranging from 150 mL up to 250 mL. These devices accomplish typical buffer exchange in 8–24 hours and are available in 2K, 3.5K, 10K, and 20K MWCOs.

Use Slide-A-Lyzer dialysis flasks in various applications, including low molecular-weight contaminant and salt removal, buffer exchange, and sample concentration.

Learn more about Slide-A-Lyzer flasks

Choose your dialysis flask

Slide-A-Lyzer dialysis cassettes of various sizes

The original Slide-A-Lyzer dialysis cassettes facilitate the rapid and effective dialysis of sample volumes ranging from 100 μL to 30 mL in 2K, 3.5K, 7K, 10K, 20K MWCOs.

Use the original Slide-A-Lyzer dialysis cassettes for applications including low-molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration.

Choose your Slide-A-Lyzer dialysis cassette based on MWCO

Learn more about Slide-A-Lyzer cassettes

Table 1. Quantitative sample recovery.

Three sample volume batches of water (0.5 mL, 1.7 mL, and 3.0 mL) were loaded and recovered per the respective manufacturer’s instructions in a Slide-A-Lyzer Dialysis Cassette or conventional dialysis tubing to determine the volumes of recovery. Water volume recovered was determined gravimetrically.

Sample volume loadedSlide-A-Lyzer dialysis cassette % volume recoveryTraditional dialysis tubing % volume recovery
3.0 mL9.4792.32
1.7 mL99.3093.12
0.5 mL98.7687.51
PB2260_slide-a-lyzer_cassette_procedure1

1. Insert syringe needle through the gasket via one of the corner ports. Inject the sample, withdraw the excess air, and remove the syringe.

PB2260_slide-a-lyzer_cassette_procedure2

2. Attach a float buoy and dialyze. (Buoys also serve as convenient bench-top stands for the cassettes).

PB2260_slide-a-lyzer_cassette_procedure3

3. Insert empty syringe needle at a second corner port. Inject air to expand the cassette chamber, and then withdraw the dialyzed sample.

Figure 1. Slide-A-Lyzer dialysis cassette workflow

Slide-A-Lyzer dialysis cassettes

What are dialysis cassettes?

Dialysis cassettes offer a more convenient and secure alternative to traditional dialysis tubing.The Slide-A-Lyzer G3 dialysis cassette offers a rigid-framed, free-standing, and self-floating vessel with a secure cap closure and large surface area consisting of regenerated cellulose membranes for simple and efficient dialysis experiments. Samples can be loaded and retrieved easily using a serological pipette.

In contrast, traditional dialysis membrane tubing presents a number of challenges: the tubing is slippery when wet and requires knots or plastic clips to secure the sample. Leakage or contamination of the sample is likely because the membrane must be closed manually (see comparison table below).

Additional features of the Slide-A-Lyzer G3 dialysis cassettes:

  • Sample security—Sealed membranes prevent leakage that can occur with dialysis tubing
  • Ergonomic—A lighter and more compact frame enables easier handling, loading, and retrieval
  • Greener by design—The Slide-A-Lyzer G3 features up to a 68% reduction in plastic waste compared to previous generations while offering the same secure, high-performance dialysis. Refer to the  Slide-A-Lyzer G3 green fact sheet for more information.
  • Self-flotation—No external flotation device or clips are required

How do Slide-A-Lyzer G3 dialysis cassettes work?

The Slide-A-Lyzer G3 is simple to use. Load the cassette with sample, put it into the dialysis buffer, and then retrieve the sample.

workflow to use G3 dialysis cassettes includes loading sample, removing any air, dialyzing, then retrieving the sample using a pipet

Figure 2. Slide-A-Lyzer G3 dialysis cassettes workflow

Slide-A-Lyzer G3 dialysis cassettes video

Thermo Scientific Slide-A-Lyzer G3 Dialysis Cassettes

The G3 dialysis cassettes offer a more convenient and secure approach to traditional dialysis tubing—just hydrate the membrane, remove the cap, load your sample, recap, dialyze, remove pressure if needed, and retrieve your dialyzed sample. View the step-by-step instructional video that shows how to use the G3 dialysis cassettes.

Comparison of Slide-A-Lyzer cassettes with traditional dialysis tubing

 
Dialysis productTraditional tubingCassette
Sample handlingSlippery when wet
Flat tubing is difficult to handle when wet, increasing risk of sample loss during sample addition/removal
Easy handling and secure sample delivery
Plastic frame and cap allow for secure sample addition/removal
Sample recoveryRisk of sample loss
Sample can leak out if clamp becomes loose or falls off
Sample protection
Welded membrane and leak-proof cap helps minimize the risk of sample loss
SpeedTime consuming
Non-optimized design that typically requires overnight dialysis
Fast and efficient
Innovative design with high surface/volume ratio allows for more rapid dialysis than conventional tubing

Slide-A-Lyzer MINI dialysis devices

Slide-A-Lyzer MINI Dialysis Devices are disposable polypropylene cups with integrated, low-binding membranes for convenient, high-performance dialysis of 10 µL to 2 mL samples. The MINI devices are designed to allow easy sample addition and removal using a serological pipette. They are effective and convenient for performing typical sample desalting or buffer exchange in 4–8 hours. Slide-A-Lyzer MINI Dialysis Devices are available in three sizes (0.1, 0.5 and 2 mL capacities) with five molecular weight cut-off (MWCO) specifications: 2K, 3.5K, 7K, 10K, and 20K.

Additional features of Slide-A-Lyzer MINI dialysis devices:

  • High sample recovery—Low-binding membranes help ensure that proteins and other macromolecules are fully recovered
  • Ease-of-use—Disposable; no syringe adapters or other assembled equipment required
  • Chemical compatibility—Cellulose membranes are compatible with common chemicals and buffers

How do Slide-A-Lyzer MINI dialysis devices work?

The Slide-A-Lyzer MINI device makes dialyzing small volumes easy. Just add the sample, place the device in the conical tube (for 0.5 and 2 mL devices), recap, and shake for up to 4 hours or overnight. Use a pipet to recover the sample (Figure 3).

workflow to use the mini dialysis device includes adding the sample, placing in a conical tube, dialyzing, then retrieving the sample by pipetting

Figure 3. Slide-A-Lyzer MINI dialysis device workflow

Table 2. High protein recovery is obtained using the 2 mL Thermo Scientific Slide-A-Lyzer MINI Dialysis Device.†

Membrane MWCOProtein/PeptideRecovery (%)
3.5 KInsulin Chain B (3.5 kDa)90.13
10 KCytochrome C (12.4 kDa)94.44
20 KMyoglobin (17 kDa)95

† Insulin chain B, cytochrome C and myoglobin (0.25 mg/mL) in either 50mM sodium phosphate, 75 mM NaCl at pH 7.2 or 0.2 M carbonate-bicarbonate buffer at pH 9.4 were dialyzed overnight (17 hours) at 4°C. The amount of protein in the retentate was determined using the Pierce BCA Protein Assay.

Slide-A-Lyzer MINI dialysis devices video

Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices

Dialyze small volume samples of 10 µL–2 mL using MINI dialysis devices. Just add your sample, place in the conical tube, cap, gently shake, and recover your dialyzed sample. View the step-by-step instructional video that shows how to use the MINI dialysis devices.

Slide-A-Lyzer dialysis flasks

Slide-A-Lyzer dialysis flasks are excellent for sample volumes ranging from 150–250 mL. They are constructed from 2 sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of an inert plastic body. The membrane and flask materials are compatible with most laboratory buffers.

With Slide-A-Lyzer dialysis flasks, typical low-molecular weight contaminant removal, buffer exchange, and desalting can be accomplished in 8 hours to overnight. The rate of dialysis depends on a variety of factors, including sample volume, size and the shape of the molecule being dialyzed, agitation, and temperature. Minimum dialysis time must be determined empirically for each specific system.

Slide-A-Lyzer dialysis flasks are manufactured using clean-room conditions, and contain low protein-binding regenerated cellulose membranes to enable maximum sample recovery and sample purity. The dialysis flasks are available in distinct colors that correspond to the MWCO of the dialysis membrane: purple (20 kDa), orange (10 kDa), pink (3.5 kDa), and blue (2 kDa).

Additional features of Slide-A-Lyzer dialysis flasks:

  • Ease-of-use—No knots or clips are needed to seal the units; simply pipette or pour sample into the flask and begin dialysis
  • High recovery—A rectangular flask design maximizes recovery of the entire sample volume via the opening at the top of the flask

How do Slide-A-Lyzer dialysis flasks work?

Slide-A-Lyzer Dialysis flasks make sample loading and recovery easy. Simply attach the supplied float-ring and hydrate membrane for 2 minutes, pour sample into device, remove air, and replace the cap. Dialyze for 8 hours to overnight. Replace buffer after 2 and 5 hours, then pour out sample to recover.

workflow to use dialysis flasks includes hydrating the membrane, adding the sample, removing any air, dialyzing, then retrieving the sample by pouring or pipetting

Figure 4. Slide-A-Lyzer dialysis flasks workflow

Slide-A-Lyzer dialysis flasks video

Thermo Scientific Slide-A-Lyzer Dialysis Flasks

Using the dialysis flasks, you can dialyze large volumes up to 250 mL. Just hydrate the membrane, add your sample, remove excess air and recap the flask, dialyze, remove pressure if needed, and recover your sample. View the step-by-step instructional video that shows how to use the dialysis flasks.

For Research Use Only. Not for use in diagnostic procedures.