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Slide-A-Lyzer Dialysis Cassettes, 10K MWCO


Faster and simpler than dialysis tubing, and with better sample recovery.

Slide-A-Lyzer Dialysis Cassettes-10K MWCO

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.

Available in four different cassette sizes to accommodate sample volumes between 0.1 and 30mL, these devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Highlights:

  • Easy to use – no knots or clamps are needed; just inject sample into cassette and begin dialysis
  • Fast dialysis – flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
  • High recovery – rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
  • Four cassette sizes – select the cassette that best suits your sample volume
  • Color-coded – orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
  • Gamma-irradiated option – gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination
Video of the Slide-A-Lyzer Cassette in action!

10K Dialysis Membrane Specifications:

  • Composition: Regenerated cellulose synthesized by the Viscose method
  • Thickness: 0.9 to 1.2mil (22.5 to 30µm)
  • Hydration Required Before Use: 30 seconds for small-volume samples
  • Glycerol Content: 21%
  • Sulfur Content: 0.05%
  • Heavy Metals Content: Trace

Gamma-irradiated Dialysis Cassettes:

Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Product Details:

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

Injection of sample into Slide-A-Lyzer Dialysis Cassette    Buffer exchange of sample and dialysate    Collection of sample from Slide-A-Lyzer Dialysis Cassette after dialysis
1. Insert syringe needle through the gasket via one of the corner ports. Inject the sample, withdraw the excess air and remove the syringe. 2. Attach a float buoy and dialyze. (Buoys also serve as convenient bench-top stands for the cassettes.) 3. Insert empty syringe needle at a second corner port. Inject air to expand the cassette chamber, then withdraw the dialyzed sample.

Slide-A-Lyzer Dialysis Cassette procedure summary. The particular cassette model shown here is the 10K MWCO (orange), 3-12mL model. Syringes and float-buoys are available separately.

 

More Product Data

Product References:

  1. Ayalew, S., Confer, A.W., and Blackwood, E.R. (2004) Characterization of immunodominant and potentially protective epitopes of Mannheimia haemolytica serotype 1 outer membrane lipoprotein PlpE. Infec. Immunity 72(12), 7265-7274.
  2. Gembitsky, D.S. et al. (2004) A prototype antibody microarray platform to monitor changes in protein tyrosine phosphorylation. Mol. Cell. Proteomics 3, 1102-1118.
  3. Albarran, B., To, R., and Stayton, P.S. (2005) A TAT-streptavidin fusion protein directs uptake of biotinylated cargo into mammalian cells. Protein Engineering, Design, & Selection 18(3), 147-152.
  4. Cole, N.B. et al. (2005) Metal-catalyzed oxidation of alpha-synuclein. J. Biol. Chem. 280(10), 9678-9690.
  5. Li, R. et al. (2005) The protein phosphatases of Synechocystis sp. Strain PCC 6803: open reading frames sll1033 and sll1387 encode enzymes that exhibit both protein-serine and protein-tyrosine phosphatase activity in vitro. J. Bacteriol. 187(17), 5877-5884.
  6. McGee, D.J. et al. (2005) Helicobacter pylori thioredoxin is an arginase chaperone and guardian against oxidative and nitrosative stresses. J. Biol. Chem. 281(6), 3290-3296.
  7. Moreland, R.J. et al. (2005) Lysosomal acid alpha-glucosidase consists of four different peptides processed from a single chain precursor. J. Biol. Chem. 280(8), 6780-6791.
  8. Polster, B.M. et al. (2005) Calpain I induces cleavage and release of apoptosis-inducing factor from isolated mitochondria. J. Biol. Chem. 280(8), 6447-6454.
  9. Torres, M. et al. (2005) Variable-region-identical antibodies differing in isotype demonstrate differences in fine specificity and idiotype. J. Immunol. 174, 2132-2142.
  10. Yang, G. et al. (2005) Activation-induced deaminase cloning, localization, and protein extraction from young VH-mutant rabbit appendix. Proc. Natl. Acad. Sci. 102(47), 17083-17088.

Related Resources:

Slide-A-Lyzer Dialysis Cassette FAQ's
Slide-A-Lyzer Dialysis Cassette dimensions
Perform labeling and other reactions in the Slide-A-Lyzer Cassette
Review of dialysis methods for protein research (and Technical Handbook)

Related Products:

Accessories for Slide-A-Lyzer Dialysis Cassettes – syringes, floats, etc.
Other Slide-A-Lyzer Cassettes – 2K, 3.5K, 7K, 10K, 20K membranes
G2 Slide-A-Lyzer Dialysis Cassettes – self-floating, pipette-loadable!
All Dialysis ProductsSelection Guide


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
66383 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 0.5mL
Formulation: Cellulose membrane framed in inert plastic
Sufficient For: 10 samples, each 0.1 to 0.5mL
10 cassettes Product Instructions for product #66383 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 0.5mL MSDS for product #66383 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 0.5mL Certificate of Analysis for product #66383 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 0.5mL $88.00

Add to cart
66383B Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 0.5mL Custom MSDS for product #66383B Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 0.5mL
66384 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 0.5mL
Formulation: Cellulose membrane framed in inert plastic
Sufficient For: 50 samples, each 0.1 to 0.5mL
5 x 10 cassettes $417.00

Add to cart
66385 Slide-A-Lyzer Dialysis Cassette Kit, 10K MWCO, 0.5mL
Formulation: Cellulose membrane framed in inert plastic
Sufficient For: 10 samples, each 0.1 to 0.5mL


Kit Contents:
Slide-A-Lyzer Cassettes, 10 ea
Slide-A-Lyzer Float Buoy, 10 ea
1mL Syringe, 10 ea
18-gauge Needle, 10 ea
10-cassette kit MSDS for product #66385 Slide-A-Lyzer Dialysis Cassette Kit, 10K MWCO, 0.5mL $109.00

Add to cart
66380 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 3mL
Formulation: Cellulose membrane framed in inert plastic
Sufficient For: 10 samples, each 0.5 to 3mL
10 cassettes Product Instructions for product #66380 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 3mL MSDS for product #66380 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 3mL Certificate of Analysis for product #66380 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 3mL $88.00

Add to cart
66381 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 3mL
Formulation: Cellulose membrane framed in inert plastic
Sufficient For: 50 samples, each 0.5 to 3mL
5 x 10 cassettes $417.00

Add to cart
66382 Slide-A-Lyzer Dialysis Cassette Kit, 10K MWCO, 3mL
Formulation: Cellulose membrane framed in inert plastic
Sufficient For: 10 samples, each 0.5 to 3mL


Kit Contents:
Slide-A-Lyzer Cassettes, 10 ea
Slide-A-Lyzer Float Buoy, 10 ea
5mL Syringe, 10 ea
18-gauge Needle, 10 ea
10-cassette kit MSDS for product #66382 Slide-A-Lyzer Dialysis Cassette Kit, 10K MWCO, 3mL Certificate of Analysis for product #66382 Slide-A-Lyzer Dialysis Cassette Kit, 10K MWCO, 3mL $125.00

Add to cart
66405 Slide-A-Lyzer Dialysis Cassette Trial Kit, 10K MWCO, 3mL
Formulation: Cellulose membrane framed in inert plastic
Sufficient For: 1 sample of 0.5 to 3mL


Kit Contents:
Slide-A-Lyzer Cassettes, 1 ea
Slide-A-Lyzer Float Buoy, 1 ea
5mL Syringe, 1 ea
18-gauge Needle, 1 ea
1-cassette kit Product Instructions for product #66405 Slide-A-Lyzer Dialysis Cassette Trial Kit, 10K MWCO, 3mL MSDS for product #66405 Slide-A-Lyzer Dialysis Cassette Trial Kit, 10K MWCO, 3mL $35.00

Add to cart
66810 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 12mL
Formulation: Cellulose membrane framed in inert plastic
Sufficient For: 8 samples, each 3 to 12mL
8 cassettes Product Instructions for product #66810 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 12mL MSDS for product #66810 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 12mL Certificate of Analysis for product #66810 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 12mL $115.00

Add to cart
66811 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 12mL
Formulation: Cellulose membrane framed in inert plastic
Sufficient For: 40 samples, each 3 to 12mL
5 x 8 cassettes $530.00

Add to cart
66807 Slide-A-Lyzer Dialysis Cassette Kit, 10K MWCO, 12mL
Formulation: Cellulose membrane framed in inert plastic
Sufficient For: 8 samples, each 3 to 12mL


Kit Contents:
Slide-A-Lyzer Cassettes, 8 ea
Slide-A-Lyzer Float Buoy, 8 ea
20mL Syringe, 10 ea
18-gauge Needle, 10 ea
8-cassette kit MSDS for product #66807 Slide-A-Lyzer Dialysis Cassette Kit, 10K MWCO, 12mL Certificate of Analysis for product #66807 Slide-A-Lyzer Dialysis Cassette Kit, 10K MWCO, 12mL $142.00

Add to cart
66830 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 30mL
Formulation: Cellulose membrane framed in inert plastic
Sufficient For: 6 samples, each 12 to 30mL
6 cassettes Product Instructions for product #66830 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 30mL MSDS for product #66830 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 30mL Certificate of Analysis for product #66830 Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 30mL $93.00

Add to cart
66454 Slide-A-Lyzer Dialysis Cassettes, gamma-irradiated, 10K MWCO, 0.5mL
Formulation: Cellulose membrane framed in inert plastic
Sufficient For: 10 samples, each 0.1 to 0.5mL
10 cassettes Product Instructions for product #66454 Slide-A-Lyzer Dialysis Cassettes, gamma-irradiated, 10K MWCO, 0.5mL MSDS for product #66454 Slide-A-Lyzer Dialysis Cassettes, gamma-irradiated, 10K MWCO, 0.5mL Certificate of Analysis for product #66454 Slide-A-Lyzer Dialysis Cassettes, gamma-irradiated, 10K MWCO, 0.5mL $121.00

Add to cart
66455 Slide-A-Lyzer Dialysis Cassettes, gamma-irradiated, 10K MWCO, 3mL
Formulation: Cellulose membrane framed in inert plastic
Sufficient For: 10 samples, each 0.5 to 3mL
10 cassettes Product Instructions for product #66455 Slide-A-Lyzer Dialysis Cassettes, gamma-irradiated, 10K MWCO, 3mL MSDS for product #66455 Slide-A-Lyzer Dialysis Cassettes, gamma-irradiated, 10K MWCO, 3mL Certificate of Analysis for product #66455 Slide-A-Lyzer Dialysis Cassettes, gamma-irradiated, 10K MWCO, 3mL $131.00

Add to cart
66453 Slide-A-Lyzer Dialysis Cassettes, gamma-irradiated, 10K MWCO, 12mL
Formulation: Cellulose membrane framed in inert plastic
Sufficient For: 8 samples, each 3 to 12mL
8 cassettes Product Instructions for product #66453 Slide-A-Lyzer Dialysis Cassettes, gamma-irradiated, 10K MWCO, 12mL MSDS for product #66453 Slide-A-Lyzer Dialysis Cassettes, gamma-irradiated, 10K MWCO, 12mL Certificate of Analysis for product #66453 Slide-A-Lyzer Dialysis Cassettes, gamma-irradiated, 10K MWCO, 12mL $122.00

Add to cart
66456 Slide-A-Lyzer Dialysis Cassettes, gamma-irradiated, 10K MWCO, 30mL
Formulation: Cellulose membrane framed in inert plastic
Sufficient For: 6 samples, each 12 to 30mL
6 cassettes Product Instructions for product #66456 Slide-A-Lyzer Dialysis Cassettes, gamma-irradiated, 10K MWCO, 30mL MSDS for product #66456 Slide-A-Lyzer Dialysis Cassettes, gamma-irradiated, 10K MWCO, 30mL Certificate of Analysis for product #66456 Slide-A-Lyzer Dialysis Cassettes, gamma-irradiated, 10K MWCO, 30mL $97.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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