Thermo Scientific SILAC Cell Culture Media and dialyzed fetal bovine serum (FBS) are optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).
Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of "heavy" 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.
- Flexible – liquid media deficient in both L-lysine and L-arginine and powdered media deficient in L-leucine, L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
- Compatible – DMEM, MEM, RPMI-1640, IMDM, Ham's F12 and McCoy's 5A media and dialyzed FBS are used to culture a variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, K562 and Jurkat
- High quality – media are sterile, endotoxin-free and cell culture-compatible
These SILAC media and dialyzed FBS serum supplements are used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.
SILAC Protein Quantitation Kit FAQ
Tech Tip #60: Prepare SILAC peptides using the In-Gel Tryptic Digestion Kit
Review of Quantitative Proteomics
Service Providers that use our SILAC Reagents
SILAC Protein Quantitation Kits – multiple kits for general use and specific cell types
Heavy and Light Amino Acids for SILAC – heavy and/or light arginine, lysine, leucine and proline
Silver Stain for Mass Spectrometry – exceptional sensitivity and recoverability for MS
In-Gel Trypsin Digest Kit – optimized kit for protein MS sample preparation