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SILAC Media and Sera


Special media for stable isotope labeling with amino acids in cell culture.

Culture media and serum for SILAC

Thermo Scientific SILAC Cell Culture Media and dialyzed fetal bovine serum (FBS) are optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).

Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of "heavy" 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Flexible – liquid media deficient in both L-lysine and L-arginine and powdered media deficient in L-leucine, L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
  • Compatible – DMEM, MEM, RPMI-1640, IMDM, Ham's F12 and McCoy's 5A media and dialyzed FBS are used to culture a variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, K562 and Jurkat
  • High quality – media are sterile, endotoxin-free and cell culture-compatible

Product Details:

These SILAC media and dialyzed FBS serum supplements are used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.

Related Resources:

SILAC Protein Quantitation Kit FAQ
Tech Tip #60: Prepare SILAC peptides using the In-Gel Tryptic Digestion Kit
Review of Quantitative Proteomics
Service Providers that use our SILAC Reagents

Related Products:

SILAC Protein Quantitation Kits – multiple kits for general use and specific cell types
Heavy and Light Amino Acids for SILAC – heavy and/or light arginine, lysine, leucine and proline
Silver Stain for Mass Spectrometry – exceptional sensitivity and recoverability for MS
In-Gel Trypsin Digest Kit – optimized kit for protein MS sample preparation


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
89984 RPMI Media for SILAC
Formulation: RPMI-1640 media minus L-Lysine and L-Arginine
Sufficient For: Part of 1 SILAC experiment
500mL MSDS for product #89984 RPMI Media for SILAC Certificate of Analysis for product #89984 RPMI Media for SILAC $46.00

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88421 RPMI Media for SILAC
Formulation: RPMI-1640 media minus L-Lysine and L-Arginine
Sufficient For: 1 to 3 SILAC experiments
6 x 500mL MSDS for product #88421 RPMI Media for SILAC Certificate of Analysis for product #88421 RPMI Media for SILAC $190.00

Add to cart
88426 Powdered RPMI Media for SILAC
Formulation: RPMI media minus L-Leucine, L-Lysine and L-Arginine
Sufficient For: Preparing 10L media for approx. 10 SILAC experiments
104g MSDS for product #88426 Powdered RPMI Media for SILAC Certificate of Analysis for product #88426 Powdered RPMI Media for SILAC $225.00

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89985 DMEM Media for SILAC
Formulation: DMEM media minus L-Lysine and L-Arginine
Sufficient For: Part of 1 SILAC experiment
500mL MSDS for product #89985 DMEM Media for SILAC Certificate of Analysis for product #89985 DMEM Media for SILAC $46.00

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88420 DMEM Media for SILAC
Formulation: DMEM media minus L-Lysine and L-Arginine
Sufficient For: 1 to 3 SILAC experiments
6 x 500mL MSDS for product #88420 DMEM Media for SILAC Certificate of Analysis for product #88420 DMEM Media for SILAC $190.00

Add to cart
88425 Powdered DMEM Media for SILAC
Formulation: DMEM media minus L-Leucine, L-Lysine and L-Arginine
Sufficient For: Preparing 10L media for approx. 10 SILAC experiments
135g MSDS for product #88425 Powdered DMEM Media for SILAC Certificate of Analysis for product #88425 Powdered DMEM Media for SILAC $225.00

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88422 MEM for SILAC
Formulation: MEM media minus L-Lysine and L-Arginine
Sufficient For: Part of 1 SILAC experiment
500mL MSDS for product #88422 MEM for SILAC Certificate of Analysis for product #88422 MEM for SILAC $51.00

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88214 Phenol Red Free MEM for SILAC
Formulation: MEM media minus phenol red, L-Lysine and L-Arginine
Sufficient For: Part of 1 SILAC experiment
500mL MSDS for product #88214 Phenol Red Free MEM for SILAC Certificate of Analysis for product #88214 Phenol Red Free MEM for SILAC $58.00

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88215 DMEM:F12 (1:1) Media for SILAC
Formulation: DMEM:F12 (1:1) media minus L-Lysine and L-Arginine for induced pluripotent cells
Sufficient For: Part of 1 SILAC experiment
500mL MSDS for product #88215 DMEM:F12 (1:1) Media for SILAC Certificate of Analysis for product #88215 DMEM:F12 (1:1) Media for SILAC $51.00

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88424 Ham's F12 for SILAC
Formulation: Ham's F12 media minus L-Lysine and L-Arginine
Sufficient For: Part of 1 SILAC experiment
500mL MSDS for product #88424 Ham's F12 for SILAC Certificate of Analysis for product #88424 Ham's F12 for SILAC $51.00

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88423 IMDM for SILAC
Formulation: IMDM media minus L-Lysine and L-Arginine
Sufficient For: Part of 1 SILAC experiment
500mL MSDS for product #88423 IMDM for SILAC Certificate of Analysis for product #88423 IMDM for SILAC $51.00

Add to cart
88441 McCoy's 5A Media for SILAC
Formulation: McCoy's 5A media minus L-Lysine and L-Arginine
Sufficient For: Part of 1 SILAC experiment
500mL MSDS for product #88441 McCoy's 5A Media for SILAC Certificate of Analysis for product #88441 McCoy's 5A Media for SILAC $51.00

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89986 Dialyzed FBS for SILAC
Formulation: Dialyzed fetal bovine serum
Sufficient For: Part of 1 SILAC experiment
50mL MSDS for product #89986 Dialyzed FBS for SILAC Certificate of Analysis for product #89986 Dialyzed FBS for SILAC $99.00

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88212 Dialyzed FBS for SILAC
Formulation: Dialyzed fetal bovine serum
Sufficient For: Part of 1 SILAC experiment
100mL MSDS for product #88212 Dialyzed FBS for SILAC Certificate of Analysis for product #88212 Dialyzed FBS for SILAC $154.00

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88440 Dialyzed FBS for SILAC
Formulation: Dialyzed fetal bovine serum
Sufficient For: Preparing media for approx. 5 SILAC experiments
500mL MSDS for product #88440 Dialyzed FBS for SILAC Certificate of Analysis for product #88440 Dialyzed FBS for SILAC $431.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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