Update profile
    Change password
    Sign out

Our site will automatically redirect starting in March. Bookmark our protein biology page on lifetechnologies.com  Go now >


A very short, NHS-ester and iodoacetyl crosslinker for amine-to-sulfhydryl conjugation.

Pierce SIA

Thermo Scientific Pierce SIA is a very short (1.5 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.


  • Reactive groups: NHS ester and iodoacetyl
  • Reactive toward: amino and sulfhydryl groups
  • Shortest sulfhydryl-reactive and amine-reactive heterobifunctional crosslinker available; compare to SIAB
  • NHS ester reacts with primary amines at pH 7-9 to form a stable amide bond
  • Iodoacetyl group reacts with sulfhydryl groups at pH > 7.5 to form stable thioether bond
  • Non-cleavable
  • Water-insoluble (dissolve first in DMF or DMSO)

Product Details:

 Succinimidyl iodoacetate; SIA
Chemical structure of SIA crosslinking reagent.


  1. Thorpe, P.E., et al. (1984). Blockade of the galactose-binding sites of ricin by its linkage to antibody. Specific cytotoxic effects of the conjugates. Eur. J. Biochem 140, 63-71.
  2. Rector, E.S., et al. (1978). A method for the preparation of protein-protein conjugates of predetermined composition. J. Immunol. Methods 24, 321-336.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
22349 SIA
Formulation: Succinimidyl iodoacetate; solid
Sufficient For: 177mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22349 SIA MSDS for product #22349 SIA Certificate of Analysis for product #22349 SIA $60.00

Now available on lifetechnologies.com

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

You may also need
34095 - SuperSignal West Femto Maximum Sensitivity Substrate

34096 - SuperSignal West Femto Maximum Sensitivity Substrate

34094 - SuperSignal West Femto Substrate Trial Kit

66330 - Slide-A-Lyzer Dialysis Cassettes, 3.5K MWCO, 3mL

24510 - Sulfo-NHS


Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2015 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747