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Secondary Antibody Coated Plates


Capture antibodies for plate assays without interference from carrier proteins.

Pierce Antibody Coated Plates

Thermo Scientific Pierce Antibody Coated Plates are ideal for binding assays when available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates.

Because these plates are IgG-specific, purified antibodies are not required. Pierce Antibody Coated Plates may be used for direct, indirect, competitive or sandwich assays and are available in clear for colorimetric assays, white for chemiluminescent assays, and black for fluorescent assays.

Highlights:

  • Prevents antibody denaturation as a result of direct binding to polystyrene
  • Unlike Protein A or Protein G Plates, these plates bind only to target species
  • Antibody-binding capacity is higher than direct adsorption onto polystyrene
  • Pre-blocked to reduce nonspecific binding
  • Anti-mouse plates: Binding Capacity: ~7pmol IgG/well
  • Anti-rabbit plates: Binding Capacity: ~12pmol IgG/well
  • Activation level: 100µL/well

References:

  1. Gartner, W., et al. (2001). Cereb Cortex. 11, 1161-1169.
  2. Wagner, L., et al. (2000). J. Biol. Chem. 275, 24740-24751.

Related Resources:

Assay development and ELISA technical guide

Related Products:

Protein A, G and L Coated Plates
Pierce 96-Well Microplates and Accessories


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
15134 Goat Anti-Mouse IgG Antibody Coated Plates, Clear, 96-Well
Formulation: Clear polystyrene 96-well plates coated at 100μL/well with goat anti-mouse IgG antibody and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 7pmol mouse IgG per well
5 plates Product Instructions for product #15134 Goat Anti-Mouse IgG Antibody Coated Plates, Clear, 96-Well MSDS for product #15134 Goat Anti-Mouse IgG Antibody Coated Plates, Clear, 96-Well Certificate of Analysis for product #15134 Goat Anti-Mouse IgG Antibody Coated Plates, Clear, 96-Well $161.00

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15234 Goat Anti-Mouse IgG Antibody Coated Plates, White, 96-Well
Formulation: White polystyrene 96-well plates coated at 100μL/well with goat anti-mouse IgG antibody and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 7pmol mouse IgG per well
5 plates Product Instructions for product #15234 Goat Anti-Mouse IgG Antibody Coated Plates, White, 96-Well MSDS for product #15234 Goat Anti-Mouse IgG Antibody Coated Plates, White, 96-Well Certificate of Analysis for product #15234 Goat Anti-Mouse IgG Antibody Coated Plates, White, 96-Well $171.00

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15334 Goat Anti-Mouse IgG Antibody Coated Plates, Black, 96-Well
Formulation: Black polystyrene 96-well plates coated at 100μL/well with goat anti-mouse IgG antibody and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 7pmol mouse IgG per well
5 plates Product Instructions for product #15334 Goat Anti-Mouse IgG Antibody Coated Plates, Black, 96-Well MSDS for product #15334 Goat Anti-Mouse IgG Antibody Coated Plates, Black, 96-Well Certificate of Analysis for product #15334 Goat Anti-Mouse IgG Antibody Coated Plates, Black, 96-Well $171.00

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15135 Goat Anti-Rabbit IgG Antibody Coated Plates, Clear, 96-Well
Formulation: Clear polystyrene 96-well plates coated at 100μL/well with goat anti-rabbit IgG antibody and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 12pmol rabbit IgG per well
5 plates Product Instructions for product #15135 Goat Anti-Rabbit IgG Antibody Coated Plates, Clear, 96-Well MSDS for product #15135 Goat Anti-Rabbit IgG Antibody Coated Plates, Clear, 96-Well Certificate of Analysis for product #15135 Goat Anti-Rabbit IgG Antibody Coated Plates, Clear, 96-Well $161.00

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15135B Goat Anti-Rabbit IgG Antibody Coated Plates, Clear, 96-Well
Formulation: Clear polystyrene 96-well plates coated at 100µL/well with goat anti-rabbit IgG antibody and blocked at 200µL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 12pmol rabbit IgG per well
Custom MSDS for product #15135B Goat Anti-Rabbit IgG Antibody Coated Plates, Clear, 96-Well Certificate of Analysis for product #15135B Goat Anti-Rabbit IgG Antibody Coated Plates, Clear, 96-Well
15136 Goat Anti-Rabbit IgG Antibody Coated Plates, White, 96-Well
Formulation: White polystyrene 96-well plates coated at 100μL/well with goat anti-rabbit IgG antibody and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 12pmol rabbit IgG per well
5 plates Product Instructions for product #15136 Goat Anti-Rabbit IgG Antibody Coated Plates, White, 96-Well MSDS for product #15136 Goat Anti-Rabbit IgG Antibody Coated Plates, White, 96-Well Certificate of Analysis for product #15136 Goat Anti-Rabbit IgG Antibody Coated Plates, White, 96-Well $171.00

Add to cart
15137 Goat Anti-Rabbit IgG Antibody Coated Plates, Black, 96-Well
Formulation: Black polystyrene 96-well plates coated at 100μL/well with goat anti-rabbit IgG antibody and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 12pmol rabbit IgG per well
5 plates Product Instructions for product #15137 Goat Anti-Rabbit IgG Antibody Coated Plates, Black, 96-Well MSDS for product #15137 Goat Anti-Rabbit IgG Antibody Coated Plates, Black, 96-Well Certificate of Analysis for product #15137 Goat Anti-Rabbit IgG Antibody Coated Plates, Black, 96-Well $171.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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