Thermo Scientific SEA BLOCK Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.
Because SEA BLOCK Blocking Buffer is fish serum, it is effective in stabilizing solutions and samples for antibody-binding interactions while at the same time minimizing the possibility of cross-reaction with mammalian sample components. When normal sera cause background in immunohistochemistry (IHC), try this product. The product is also especially effective in various cellular imaging protocols based on visible and near-infrared fluorescence detection.
- Non-mammalian – fish proteins are less likely to have specific binding interactions with antibodies and other mammalian proteins present in typical methods
- Convenient – filtered and stabilized in PBS for compatibility with most assay systems
- Easy to use – can be used as supplied or diluted up to 10-fold as needed
- Flexible – may be used for many different applications, including as a diluent for antibodies
Fish serum has several advantages over typical blocking buffers. Because salmon is phylogenetically distant from mammals, which are the source of antibodies and samples used in most experiments, its serum proteins are less likely to have specific binding interactions with proteins used in typical cell biology experiments. Because SEA BLOCK Blocking Buffer is fish serum, it is effective in stabilizing solutions and samples for antibody-binding interactions. When normal sera cause background in IHC experiments, SEA BLOCK Blocking Buffer may correct the problem.
SEA BLOCK Blocking Buffer is particularly effective in applications involving fluorescence imaging. The blocker has been used to decrease background and increase signal-to-noise ratios with near-IR fluorescent probes and is validated for use with the LI-COR Odyssey™ Infrared Imaging System.
- Alegria-Schaffer, A., et al. (2009). Performing and optimizing Western blots with an emphasis on chemiluminescent detection. Methods Enzymol. 463:573-99.
- Hypolite, J.A., et al. (2001). Am. J. Physiol. Cell. Physiol. 280:C254-64.
- Wang, L, et al. (2002). J. Clin. Invest. 110:1175-1184.
Blocking buffers for Western blot and ELISA -- technical guide
Blocking strategies for immunohistochemistry -- technical guide
All Blocking Buffers