Thermo Scientific Sodium Dodecyl Sulfate (Lauryl) is standard-grade SDS detergent for use in protein polyacrylamide gel electrophoresis (PAGE).
This lauryl-grade sodium dodecyl sulfate (SDS) is a popular anionic detergent for routine protein electrophoresis and cell lysis methods. The formulation is a mixture of several different alkyl sulfate chain lengths (C10 to C18).
- Popular anionic detergent for a variety of protein methods
- Especially useful for denaturing polyacrylamide gel electrophoresis (SDS-PAGE)
- Common component of cell lysis buffers
|Chemical structure of SDS detergent.
Properties of SDS (values for pure C12):
- Molecular Weight: 288.5g
- Detergent Class: Ionic (anionic)
- Aggregation Number: 62
- Micelle Molecular Weight: 18,000g
- Critical Micelle Concentration (CMC): 6 to 8mM (0.1728 to 0.2304%, w/v)
- Cloud Point: >100°C
- Dialyzable: No
Specifications for Sodium Dodecyl Sulfate (Part No. 28364, 28365):
- Visual: White powder, free of foreign material.
- Solubility: 10% (aq, w/v) solution must be clear, colorless to slightly yellow.
- Chain length: C12 >60%; C14 = 20 to 35%; C16 <10%; C10 and C18 <1% each
Specifications for SDS, 20% Solution (Part No. 62202):
- Visual: Clear, colorless liquid, free of foreign material.
- pH: 5 to 8
- DNase, RNase and Protease: None
Review of detergents for cell lysis
Detergent Sampler Pack
Other Detergents for Protein Methods
Detergent Removal Resins