Thermo Scientific Sodium Dodecyl Sulfate (C12) is a highly purified form of SDS that is especially suited for protein experiments requiring tightly controlled solubility parameters.
Sodium dodecyl sulfate (SDS) is an alkyl sulfate, anionic detergent used frequently in protein electrophoresis and protein solubilization methods. It is a component of many cell lysis buffer formulations, including RIPA buffer. Typical SDS or lauryl sulfate preparations are mixtures of several different chain lengths (C10 to C18). However, protein renaturation and other specialized methods require very specific surfactant and buffer conditions. This particular formulation of SDS is highly purified to eliminate nearly all secondary chain lengths besides C12.
- Popular anionic detergent for protein electrophoresis and solubilization
- Component of many cell lysis buffer formulations
- Specially purified formulation is >98% C12 alkyl sulfate
- Contains low levels of hexadecyl sulfate (C16), which inhibits protein renaturation
|Chemical structure of SDS detergent.
Properties of Sodium Dodecyl Sulfate, (pure C12):
- Molecular Weight: 288.5g
- Detergent Class: Ionic (anionic)
- Aggregation Number: 62
- Micelle Molecular Weight: 18,000g
- Critical Micelle Concentration (CMC): 6 to 8mM (0.1728 to 0.2304%, w/v)
- Cloud Point: >100°C
- Dialyzable: No
Specifications for Sodium Dodecyl Sulfate (SDS), C12 (Part No. 28312):
- Visual: White powder, free of foreign material.
- Solubility: 10% (aq, w/v) solution must be clear, colorless and free of particulates.
- Chain length: C12 ≥98.0% if C16 ≤0.8%
- Report C10, C12, C14, C16, C18 concentrations.
Review of detergents for cell lysis
Detergent Sampler Pack
Other Detergents for Protein Methods
Detergent Removal Resins