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A short, NHS-ester and bromoacetyl crosslinker for amine-to-sulfhydryl conjugation.

Pierce SBAP

Thermo Scientific Pierce SBAP is a short (6.2 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and bromoacetyl reactive groups.


  • Reactive groups: NHS ester and bromoacetyl
  • Reactive toward: amino and sulfhydryl groups
  • NHS ester reacts with primary amines at pH 7-9 to form a stable amide bond
  • Bromacetyl group reacts with sulfhydryl groups at pH > 7.5 to form stable thioether bonds
  • Non-cleavable
  • Water-insoluble (dissolve first in DMF or DMSO)
  • Spacer maintains peptide-like character in the crosslinked species
  • Resulting crosslink is susceptible to acid hydrolysis
  • Useful for preparing cyclic peptides and peptide conjugates

Product Details:

Succinimidyl 3-(bromoacetamido)propionate; SBAP
Chemical structure of SBAP crosslinking reagent.


  1. Inman, J.K., et al. (1991). Synthesis of N-alpha-(tertbutoxycarbonyl)-N-epsilon-[N-(bromoacetyl)-beta-alanyl]-L-lysine: Its use in peptide synthesis for placing a bromoacetyl crosslinking function at any desired sequence position. Bioconjugate Chem. 2, 458-463.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
22339 SBAP
Formulation: Succinimidyl 3-(bromoacetamido)propionate; solid
Sufficient For: 163mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22339 SBAP MSDS for product #22339 SBAP Certificate of Analysis for product #22339 SBAP $132.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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