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Pierce Saturated Ammonium Sulfate Solution


Concentrate and partially purify antibodies from dilute culture supernatants.

Pierce Saturated Ammonium Sulfate Solution

Thermo Scientific Pierce Saturated Ammonium Sulfate Solution is formulated and ready to use for simple immunoglobulin, antibody, or other protein fractionation applications based on selective precipitation, which is also called salting out.

The saturated ammonium sulfate solution (4.32M) enables removal of abundant proteins from serum, cell culture supernatant and ascites fluid. Slowly adding the solution to a protein sample results in decreased protein solubility and eventual protein precipitation as the charged ammonium sulfate ions compete with protein for water interaction. High molecular weight proteins, such as antibodies, are among the first to precipitate. When used in combination with antibody purification methods such as affinity chromatography, ammonium sulfate precipitation is ideal for achieving highly purified antibody samples.

Highlights:

  • Convenient – solution is ready to use; no preparation required
  • Concentrates – recover antibody in 100mL from more than 1L of cell culture supernatant
  • Improves purity – partial-purification of antibodies by ammonium sulfate precipitation greatly improves the final purity that can be obtained with affinity purification
  • Extends column life – using pre-purified antibody samples instead of crude samples enables affinity columns to be used for many more cycles
  • Compatible – ammonium sulfate is easily removed by dialysis or desalting columns, eliminating any obstacles to downstream methods
  • Versatile – use for precipitating immunoglobulins or "salting out" fractions of other sample types

Related Resources:

Review of antibody purification methods

Related Products:

Melon Gel Monoclonal IgG Purification Kit (for culture supernatant and ascites)
Melon Gel IgG Purification Kit (for serum samples)
Protein A, Protein G and other IgG purification resins


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
45216 Saturated Ammonium Sulfate Solution
Formulation: 4.32M solution of ammonium sulfate
Sufficient For: Precipitating antibody from 1L of serum, ascites or culture supernatant
1L Product Instructions for product #45216 Saturated Ammonium Sulfate Solution MSDS for product #45216 Saturated Ammonium Sulfate Solution Certificate of Analysis for product #45216 Saturated Ammonium Sulfate Solution $159.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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