Update profile
    Change password
    Sign out

Restore Plus Western Blot Stripping Buffer


Stripping for high-affinity antibodies that require special treatment.

Restore Plus Western Blot Stripping Buffer

Thermo Scientific Restore PLUS Western Blot Stripping Buffer is an advanced formula for removing bound primary and secondary antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Restore PLUS Buffer is an alternative formulation of the original Thermo Scientific Restore Western Blot Stripping Buffer. Restore PLUS Stripping Buffer was designed for use with antibodies that are difficult to remove from Western blots and require longer incubation times or incubation temperatures greater than 22°C with gentler formulations. High-affinity antibodies can be quickly and effectively stripped from Western blots at room temperature without removing transferred proteins, thereby allowing multiple reprobes of the target.

Highlights:

  • Ready and easy to use – no dilution necessary; no offensive odors; store at room temperature
  • Compatible – use on nitrocellulose and PVDF membranes, whether still wet or already dry; works with practically any blocking buffer, enzyme conjugate and chemiluminescent substrate
  • Cost effective – save valuable time and samples; strip blots effectively the first time
  • Robust yet gentle – transferred proteins remain viable; strip the same blot up to five times
  • Flexible – strip and reprobe to optimize antibody concentrations or to detect a new antigen with different antibodies

Product Details:


More effective membrane stripping and reprobing with Restore PLUS Western Blot Stripping Buffer.
 
Thermo Scientific Restore PLUS Western Blot Stripping Buffer is most effective for reprobing with different antibodies. HeLa cell lysate was probed for actin and detected with Thermo Scientific Pierce ECL Substrate (Original panel). Blots were then stripped with either Restore PLUS Stripping Buffer or other suppliers’ stripping buffers (Stripped panel). The blots were then re-blocked and reprobed for cyclophilin B and detected with Pierce ECL Substrate (Reprobed panel).

Protocol Summary:

  • Wash blot to remove chemiluminescent substrate
  • Incubate blot in Restore Western Blot Stripping Buffer for 5 to 15 minutes at room temperature
    (or incubate at 37°C for high affinity antibodies)
  • Remove blot and wash in Wash Buffer
  • Block membrane
  • Test for sufficient removal of antibodies
  • Perform next immunoblot experiment

General References:

  1. Baolin Zhang, B, et al. (2003). Mol. Cell. Biol. 23: 5716-5725.
  2. Kaufmann, S.H., et al. (1987). Anal. Biochem. 161: 89-95.
  3. Kaufmann, S.H. and Kellner, U. (1998). Erasure of Western blots after autoradiographic or chemiluminescent detection. In Immunochemical Protocols. Ed. Pound, J.D. Humana Press, Totowa, NJ, 223-235.
  4. Lanying Wen, L., et al. (2003). Genetics. 165: 771-779.
  5. Schrager, J.A., et al. (2002). J. Biol. Chem. 277: 6137-6142.
  6. Skurk, C., et al. (2004). J. Biol. Chem. 277: 6137-6142.
  7. Sorci, G., et al. (2003). Mol. Cell. Biol. 23: 4870-4881.

Related Products:

Restore Western Blot Stripping Buffer – original gentle formulation
Restore Fluorescent Western Blot Stripping Buffer – formulated for fluorescent blotting on PVDF
Blocking Buffers – choose from protein-based and protein-free blocking buffers
Chemiluminescent Substrates – compare substrates to the choose the best for your application
CL-XPosure Film – X-ray film for chemiluminescent detection
Background Eliminator for Film – remove excess signal from film to make data clearer


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
46428 Restore PLUS Western Blot Stripping Buffer
Formulation: Proprietary clear, colorless, acidic solution
Sufficient For: 2 mini blots or 160cm2 membrane
30mL Product Instructions for product #46428 Restore PLUS Western Blot Stripping Buffer MSDS for product #46428 Restore PLUS Western Blot Stripping Buffer Certificate of Analysis for product #46428 Restore PLUS Western Blot Stripping Buffer $37.00

Add to cart
46430 Restore PLUS Western Blot Stripping Buffer
Formulation: Proprietary clear, colorless, acidic solution
Sufficient For: 25 mini blots or 2000cm2 membrane
500mL Product Instructions for product #46430 Restore PLUS Western Blot Stripping Buffer MSDS for product #46430 Restore PLUS Western Blot Stripping Buffer Certificate of Analysis for product #46430 Restore PLUS Western Blot Stripping Buffer $127.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

You may also need
21059 - Restore Western Blot Stripping Buffer

28908 - 16% Formaldehyde (w/v), Methanol-free

28904 - Trifluoroacetic Acid (TFA), Sequencing grade

24590 - GelCode Blue Stain Reagent

88323 - Pierce LTQ Velos ESI Positive Ion Calibration Solution

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747