Stripping for high-affinity antibodies that require special treatment.
Thermo Scientific Restore PLUS Western Blot Stripping Buffer is an advanced formula for removing bound primary and secondary antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Restore PLUS Buffer is an alternative formulation of the original Thermo Scientific Restore Western Blot Stripping Buffer. Restore PLUS Stripping Buffer was designed for use with antibodies that are difficult to remove from Western blots and require longer incubation times or incubation temperatures greater than 22°C with gentler formulations. High-affinity antibodies can be quickly and effectively stripped from Western blots at room temperature without removing transferred proteins, thereby allowing multiple reprobes of the target.
Ready and easy to use – no dilution necessary; no offensive odors; store at room temperature
Compatible – use on nitrocellulose and PVDF membranes, whether still wet or already dry; works with practically any blocking buffer, enzyme conjugate and chemiluminescent substrate
Cost effective – save valuable time and samples; strip blots effectively the first time
Robust yet gentle – transferred proteins remain viable; strip the same blot up to five times
Flexible – strip and reprobe to optimize antibody concentrations or to detect a new antigen with different antibodies
Thermo Scientific Restore PLUS Western Blot Stripping Buffer is most effective for reprobing with different antibodies. HeLa cell lysate was probed for actin and detected with Thermo Scientific Pierce ECL Substrate (Original panel). Blots were then stripped with either Restore PLUS Stripping Buffer or other suppliers’ stripping buffers (Stripped panel). The blots were then re-blocked and reprobed for cyclophilin B and detected with Pierce ECL Substrate (Reprobed panel).
Wash blot to remove chemiluminescent substrate
Incubate blot in Restore Western Blot Stripping Buffer for 5 to 15 minutes at room temperature
(or incubate at 37°C for high affinity antibodies)
Remove blot and wash in Wash Buffer
Test for sufficient removal of antibodies
Perform next immunoblot experiment
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