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Red Firefly Luciferase Reporter Assay Vectors


Red firefly reporter plasmids for bright protein expression in luciferase assays.

Thermo Scientific pMCS-Red Firefly Luciferase Vector

The Thermo Scientific pMCS-Red Firefly Luc Vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the intracellular red firefly luciferase reporter.

The Red Firefly Luc Vectors contain a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum. The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential. The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively. These constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.

Highlights:

  • Intracellular Red Firefly Luciferase gene, optimized for high expression in mammalian systems
  • Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
  • Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
  • Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
  • High-copy pUC bacterial DNA replication origin
  • Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization

Product Details:

The pMCS-, pCMV- and pTK-Red Firefly Luc Vectors encode the Red Firefly luciferase reporter with excellent light intensity.

Map of pMCS-Red-Firefly Luc Vector for luciferase assays
Plasmid map of the Thermo Scientific pMCS-Red-Firefly Luc Vector. See the following downloadable files for complete vector and cloning site sequences.

 

Download the complete sequence (.txt) and printable data sheet (.pdf) of each vector.
Vector Sequence Text File Data Sheet
pMCS-Red Firefly Luc pMCS-Red Firefly Luc Sequence (.txt) Data Sheet (.pdf)
pCMV-Red Firefly Luc pCMV-Red Firefly Luc Sequence (.txt) Data Sheet (.pdf)
pTK-Red Firefly Luc pTK-Red Firefly Luc Sequence (.txt) Data Sheet (.pdf)

These vectors are subject to a limited use label license.

More Product Data

Related Resources:

Review of Luciferase Reporters
Luciferase Reporter Assay Selection Guide

Related Products:

Pierce Luciferase Assay Kits and Vectors
Other Cell Lysis Reagents
Protein Assay Reagents and Kits


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
16155 pMCS-Red Firefly Luc
Formulation: DNA at 0.5µg/µL in 10mM Tris-HCl, pH 8.5
10µg MSDS for product #16155 pMCS-Red Firefly Luc Certificate of Analysis for product #16155 pMCS-Red Firefly Luc $57.00

Add to cart
16156 pCMV-Red Firefly Luc
Formulation: DNA at 0.5µg/µL in 10mM Tris-HCl, pH 8.5
10µg MSDS for product #16156 pCMV-Red Firefly Luc Certificate of Analysis for product #16156 pCMV-Red Firefly Luc $57.00

Add to cart
16157 pTK-Red Firefly Luc
Formulation: DNA at 0.5µg/µL in 10mM Tris-HCl, pH 8.5
10µg MSDS for product #16157 pTK-Red Firefly Luc Certificate of Analysis for product #16157 pTK-Red Firefly Luc $57.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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