Update profile
    Change password
    Sign out

Rat TNF alpha ELISA Kits


Microplate kits for sandwich ELISA of rat TNF alpha (rat TNFa).

Thermo Scientific Rat TNF alpha ELISA Kits

Assay TNFα (rat tumor necrosis factor alpha) levels in rat serum and culture supernatant samples with our Thermo Scientific Rat TNF alpha ELISA Kits.

These rat TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant rat TNFα (rat tumor necrosis factor alpha)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 31 to 2500pg/mL
  • Sensitivity: < 15pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%).
Video of the Thermo Scientific TNF alpha ELISA Kit

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Rat TNF alpha ELISA standard curve
Rat TNF alpha ELISA standard curve. An example standard curve for the Thermo Scientific Rat TNF alpha ELISA Kit (Part No. ER3TNFA), with error bars based on four replicates for each tumor necrosis factor alpha standard concentration.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
ER3TNFA Rat TNF alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Rat TNF alpha Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Rat TNF alpha Standard, 2 vials
Standard Diluent, 25mL
Pre-Treatment Buffer, 6mL
Biotinylated Antibody Reagent, 6mL
Streptavidin-HRP Reagent, 14mL
TMB Substrate Solution, 13mL
30X Wash Buffer, 50mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #ER3TNFA Rat TNF alpha ELISA Kit MSDS for product #ER3TNFA Rat TNF alpha ELISA Kit $495.00

Add to cart
ER3TNFA2 Rat TNF alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Rat TNF alpha Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Rat TNF alpha Standard, 4 vials
Standard Diluent, 2 x 25mL
Pre-Treatment Buffer, 2 x 6mL
Biotinylated Antibody Reagent, 2 x 6mL
Streptavidin-HRP Reagent, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
30X Wash Buffer, 2 x 50mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #ER3TNFA2 Rat TNF alpha ELISA Kit MSDS for product #ER3TNFA2 Rat TNF alpha ELISA Kit $740.00

Add to cart
ER3TNFA5 Rat TNF alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Rat TNF alpha Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Rat TNF alpha Standard, 10 vials
Standard Diluent, 5 x 25mL
Pre-Treatment Buffer, 5 x 6mL
Biotinylated Antibody Reagent, 5 x 6mL
Streptavidin-HRP Reagent, 5 x 14mL
TMB Substrate Solution, 5 x 13mL
30X Wash Buffer, 5 x 50mL
Stop Solution, 5 x 13mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #ER3TNFA5 Rat TNF alpha ELISA Kit MSDS for product #ER3TNFA5 Rat TNF alpha ELISA Kit $1,650.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747