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Rat IL-6 ELISA Kits


Microplate kits for sandwich ELISA of rat interleukin-6 (IL-6).

Thermo Scientific Rat IL-6 ELISA Kits

Assay IL-6 (rat interleukin-6) levels in rat serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Rat IL-6 ELISA Kits.

These rat IL-6 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-6 (IL-6) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant rat interleukin-6 (IL-6)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 62.5 to 4000pg/mL
  • Sensitivity: < 16pg/mL
  • Precision: dependent upon sample type
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 4 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with rat IL-10 and TNFα; mouse IL-6, IL-1β, TNFα, IL-4, IL-2 and IL-1α; or human IL-8 and GM-CSF.
Video of the Thermo Scientific IL-6 ELISA Kit

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Product Details:

Rat IL-6 ELISA standard curve
Rat IL-6 ELISA standard curve. An example standard curve for the Thermo Scientific Rat IL-6 ELISA Kit (Part No. ER3IL6), with error bars based on four replicates for each interleukin-6 standard concentration.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
ER3IL6 Rat IL-6 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Rat IL-6 Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Rat IL-6 Standard, 2 vials
Standard Diluent, 12mL
Biotinylated Antibody Reagent, 12mL
20X Wash Buffer, 55mL
Streptavidin-HRP Concentrate, 75μL
HRP Dilution Buffer, 13mL
TMB Substrate Solution, 12mL
Stop Solution, 6mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #ER3IL6 Rat IL-6 ELISA Kit MSDS for product #ER3IL6 Rat IL-6 ELISA Kit $495.00

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ER3IL62 Rat IL-6 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Rat IL-6 Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Rat IL-6 Standard, 4 vials
Standard Diluent, 2 x 12mL
Biotinylated Antibody Reagent, 2 x 12mL
20X Wash Buffer, 2 x 55mL
Streptavidin-HRP Concentrate, 2 x 75μL
HRP Dilution Buffer, 2 x 13mL
TMB Substrate Solution, 2 x 12mL
Stop Solution, 2 x 6mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #ER3IL62 Rat IL-6 ELISA Kit MSDS for product #ER3IL62 Rat IL-6 ELISA Kit $750.00

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ER3IL65 Rat IL-6 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Rat IL-6 Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Rat IL-6 Standard, 5 vials
Standard Diluent, 55mL
Biotinylated Antibody Reagent, 55mL
20X Wash Buffer, 250mL
Streptavidin-HRP Concentrate, 250μL
HRP Dilution Buffer, 70mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 30mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #ER3IL65 Rat IL-6 ELISA Kit MSDS for product #ER3IL65 Rat IL-6 ELISA Kit $1,500.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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