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R-Phycoerythrin Fluorescent Protein


A red-fluorescent phycobiliprotein to make fluorescent molecular probes.

R-Phycoerythrin

Thermo Scientific R-Phycoerythrin is a red-fluorescent, multi-subunit protein that can be crosslinked or conjugated to other molecules to make fluorescent probes.

This fluorochrome is a member of the phycobiliprotein family of proteins isolated from the marine algae Porphyra tenera or Gastroclonium coulterii. Fluorochrome-labeled reagents have high resolution and are therefore advantageous in immunological assays, such as live cell staining, double-labeling techniques and cell sorting procedures. The strong absorption bands of the R-Phycoerythrin are in the visible region of the spectrum, extending from the green to the far-red wavelengths. The absorbance spectra extends over a broad range of potential excitation wavelengths, allowing for versatility in the excitation source and creating large Stokes shifts, thus minimizing interference from Rayleigh-scattered light.

Highlights:

  • R-Phycoerythrin – red-fluorescent, multi-subunit protein in the phycobiliprotein family
  • Large Stokes shift – excitation (absorption) at one of three wavelengths produces light emission at a much higher wavelength

Properties of R-Phycoerythrin:

Alternative names R-Phycoerythrin, R-PE, fluorescent phycobiliprotein
Molecular weight 240,000
Excitation wavelength 480, 545, 565nm
Emission wavelength 578nm

General References:

  1. Oi, V.T., et al. (1982). Fluorescent phycobiliprotein conjugates for analyses of cells and molecules. J. Cell Biol. 93, 981-986.
  2. Kronick, M.N. and Grossman, P.D. (1983). Immunoassay techniques with fluorescent phycobiliprotein conjugates. Clin. Chem. 29, 1582-1586.
  3. Parks, D.R. and Herzenberg, L.A. (1984). Fluorescence-activated cell sorting: theory, experimental optimization, and applications in lymphoid-cell biology. Meth. Enzymol. 108, 197-241.
  4. Kronick, M.N. (1986). The use of phycobiliproteins as fluorescent labels in immunoassay.
    J. Immunol. Meth. 92, 1-13.
  5. White, J.C. and Stryer, L. (1987). Photostability studies of phycobiliprotein fluorescent labels. Anal. Biochem. 161, 442-452.
  6. Festin, R., et al. (1987). Detection of triple antibody-binding lymphocytes in standard single laser flow cytometry using colloidal gold, fluorescein and phycoerythrin as labels.
    J. Immunol. Meth. 101, 23-28.

Related Products:

DyLight Fluor Labeling Reagents and Kits
Fluorescent Labeling – Top-level menu of all reagents


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
46185 R-Phycoerythrin
Formulation: Purified fluorescent protein in ammonium sulfate suspension
Sufficient For: Conjugation with approx. 1mg of antibody (IgG)
2mg Product Instructions for product #46185 R-Phycoerythrin MSDS for product #46185 R-Phycoerythrin Certificate of Analysis for product #46185 R-Phycoerythrin $312.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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