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Pierce Quantitative Peroxide Assay Kits


Measure total peroxide levels in either aqueous or lipid-containing laboratory reagents.

Pierce Quantitative Peroxide Assay Kits

Thermo Scientific Pierce Quantitative Peroxide Assay Kits detect and measure hydrogen peroxide levels in biological samples using an iron and xylenol orange (XO) reagent.

These aqueous and lipid-compatible peroxide assay kits detect peroxides based on oxidation of ferrous to ferric ion in the presence of xylenol orange. One assay kit has an aqueous-compatible formulation that includes sorbitol, which provides sensitivity enhancement. The other assay kit has a formulation that may be used with lipid-containing samples without first extracting the lipids; however, because this kit does not contain sorbitol, the assay is less sensitive. Most proteins do not interfere with these assays, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects.

Highlights:

  • Peroxides – detects and measures hydrogen peroxide (H2O2) levels in biological and other liquids samples
  • Convenient – no proprietary reagents involved but the ready-to-use kits remove the variability, difficulty and added expense of formulating the reagents yourself
  • Versatile – quantitation by calculation from the extinction coefficient or by reference to a standard curve enables the method to be easily adapted to a variety of sample types and testing purposes
  • Two formulations – choose the aqueous-compatible kit for maximum sensitivity with non-lipid samples; choose the lipid-compatible kit for best results with lipid-containing samples

Applications:

  • General hydrogen peroxide (H2O2) measurement in protein samples
  • Quantitation of peroxides in detergents
  • Assessment of the effects of peroxides on cellular activity
  • Determination of protein glycation; Measurement of peroxide accumulation in lipids

Product Details:

In these assays, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With the aqueous-compatible formulation, peroxide first reacts with sorbitol, converting it to a peroxyl radical, which in turn initiates Fe2+ oxidation to Fe3+. In the lipid compatible formulation, the peroxide converts the Fe2+ to Fe3+ directly. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560nm. Peroxide levels in test samples can be determined by calculation from the know extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.

References:

  1. Jiang, Z.-Y., Woollard, A.C.S. and Wolff, S.P. (1991). Lipid peroxide measurement by oxidation of Fe++ in the presence of Xylenol Orange: Comparison with the thiobarbituric acid assay and an iodometric method. Lipids 26, 853-856.
  2. Jiang, Z.-Y., Hunt, J.V. and Wolff, S.P. (1992). A simple Fe++-oxidation method for detection of lipid peroxide in low density lipoprotein and liposomes. Anal. Biochem. 202, 384-389.

Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
23280 Quantitative Peroxide Assay Kit (Aqueous)
Formulation: Multi-component kit
Sufficient For: 100 tube assays or 500 microplate assays with non-lipid samples

Kit Contents:
Reagent A, 1mL
Reagent B, 2 x 50mL
100mL kit Product Instructions for product #23280 Quantitative Peroxide Assay Kit (Aqueous) MSDS for product #23280 Quantitative Peroxide Assay Kit (Aqueous) Certificate of Analysis for product #23280 Quantitative Peroxide Assay Kit (Aqueous) $157.00

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23285 Quantitative Peroxide Assay Kit (Lipid)
Formulation: Multi-component kit
Sufficient For: 100 tube assays or 500 microplate assays with lipid-containing samples

Kit Contents:
Reagent A, 1mL
Reagent C, 4 x 25mL
100mL kit Product Instructions for product #23285 Quantitative Peroxide Assay Kit (Lipid) MSDS for product #23285 Quantitative Peroxide Assay Kit (Lipid) Certificate of Analysis for product #23285 Quantitative Peroxide Assay Kit (Lipid) $144.00

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23281 Peroxide Assay Reagent A
Formulation: 25mM ammonium ferrous sulfate
Sufficient For: Sufficient For: Use with aqueous- or lipid-compatible procedures
1mL MSDS for product #23281 Peroxide Assay Reagent A Certificate of Analysis for product #23281 Peroxide Assay Reagent A $53.00

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23282 Peroxide Assay Reagent B
Formulation: 125µM xylenol orange in water with sorbitol
Sufficient For: Use with aqueous-compatible procedures
50mL MSDS for product #23282 Peroxide Assay Reagent B Certificate of Analysis for product #23282 Peroxide Assay Reagent B $96.00

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23283 Peroxide Assay Reagent C
Formulation: 125µM xylenol orange in methanol with BHT
Sufficient For: Use with lipid-compatible procedures
25mL MSDS for product #23283 Peroxide Assay Reagent C Certificate of Analysis for product #23283 Peroxide Assay Reagent C $99.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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