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Proteinase K


Inactivates DNase and RNase to aid in nucleic acid purification.

Pierce Proteinase K

Thermo Scientific Pierce Proteinase K is a protease that cleaves at the carboxyl side of aliphatic, aromatic or hydrophobic residues and is commonly used to digest and inactivate DNase and RNase during in nucleic acid purification.

Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album. It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times. The activity of proteinase K is increased at elevated temperatures up to 65°C. Calcium is not essential to the function of proteinase K. Therefore, EDTA and other chelating agents do not interfere with the activity and may be used alongside proteinase K to inactivate calcium-dependent nucleases in DNA and RNA preparation.


Properties of Proteinase K Enzyme.
Alternative names Peptidase K, Tritirachium alkaline proteinase
Specificity Cleaves at the carboxyl side of aliphatic, aromatic or hydrophobic residues
Proteinase K Source Tritirachium album
Molecular weight 28,900
Form Lyophilized form
Concentration/activity >30 units/mg at 35°C
RNase/DNase RNase-free and DNase-free
Protease type Serine protease
Uses/applications Inactivation of RNase and DNase during nucleic acid purification
Reaction conditions 0.05-1 mg/ml proteinase K, pH 7.5-8, often containing 0.5-1% SDS
Storage conditions Store at -20°C
Inhibitors PMSF or DFP

References:

  1. Ebeling, W., et al. (1974) Proteinase K from Tritirachium album limber. Eur. J. Biochem. 47,
    91-97.
  2. Liu, D., et al. (2003) Endocrinology. 144, 4894-4904.

Related Products:

Pierce Trypsin, MS Grade (Part No. 90057)
MS-Grade Proteases and Digestion Kits for Mass Spectrometry
Standard-Grade Proteases and Protease Resins
Enzymes for Cell Lysis


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
17916 Proteinase K
Formulation: Lyophilized enzyme powder
Sufficient For: At least 30 units/mg
100mg MSDS for product #17916 Proteinase K Certificate of Analysis for product #17916 Proteinase K $125.00

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17916B Proteinase K
Formulation: Lyophilized enzyme powder
Sufficient For: At least 30 units/mg
Custom MSDS for product #17916B Proteinase K Certificate of Analysis for product #17916B Proteinase K

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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