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Conjugates primary amines to DNA via light-activated intercalation among pyrimidines.

Pierce SPB

Thermo Scientific Pierce SPB is an NHS-ester and psoralen crosslinker that effectively conjugates primary amines to DNA via light-activated intercalation next to pyrimidine bases.


  • Reactive groups: NHS ester and psoralen
  • Reactive toward: amino groups and thymine
  • DNA intercalating agent and nucleic acid probe1,2
  • Reagent for photo-immobilization to microplates3
  • Melting point: 177-178°C


Product Details:

Succinimidyl-[4-(psoralen-8-yloxy)]-butyrate; SPB
Chemical structure of SPB crosslinking reagent.


  1. Laskin, J.D., et al. (1986). Psoralen potentiate ultraviolet light induced inhibition of epidermal growth factor binding. Proc. Natl. Acad. Sci. USA 83, 8211.
  2. Inman, R.B. and Schnos, M. (1987). Electron microscopic identification of supercoiled regions ina complex DNA structure. J. Mol. Biol. 193, 377.
  3. Elsner, H.I. and Mouritsen, S. (1994). Use of psoralens for covalent immobilization of biomolecules in solid phase assays. Bioconjugate Chem. 5, 463-467.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)
Chromatin IP (ChIP) Kits

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
23013 SPB
Formulation: Succinimidyl-[4-(psoralen-8-yloxy)]-butyrate; solid
Sufficient For: 130mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #23013 SPB MSDS for product #23013 SPB Certificate of Analysis for product #23013 SPB $174.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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