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Protein G UltraLink Resin


Protein G beads based on a durable resin for medium pressure affinity purification of IgG.

Pierce Protein G UltraLink Resin

Thermo Scientific Pierce Protein G UltraLink Resin is an extremely durable beaded polyacrylamide support to which recombinant Protein G has been immobilized for use in IgG purification methods.

The Protein G UltraLink Resin is used to bind and purify IgG in gravity-flow, spin-column or medium-pressure chromatography methods. Protein G is a popular antibody-binding protein that is especially useful for affinity purification of IgG from mouse, human and rat serum.

Highlights:

  • Immobilized Protein G – specifically binds IgG from several mammalian species, especially all isotypes of IgG from human, mouse and rat
  • Standard capacity – loading of immobilized Protein G provides a binding capacity of approx. 20mg human IgG per milliliter of resin
  • UltraLink Resin – acrylamide-based support provides an alternative to crosslinked beaded agarose supports for medium pressure applications

Product Details:

UltraLink Resin is an azlactone-activated polyacrylamide support that is hydrophilic, charge-free, high capacity, highly crosslinked, rigid and porous. This support is especially useful for medium pressure techniques when using large sample volumes requiring fast-flow techniques (FPLC) and large-scale applications. Unlike typical beaded agarose supports, UltraLink Support does not crush or collapse when subjected to high flow rates that result in pressures exceeding 25psi.

Properties of Thermo Scientific UltraLink Resin:

  • Support pH Stability: 1 to 13
  • Average Particle Size: 50 to 80 microns
  • Exclusion Limit: greater than 2,000,000 daltons
  • Average Surface Area: greater than 250m2 per gram of beads
  • Average Pore Volume: greater than 1.2mL per gram of beads (> 60% of bead volume)
  • Pore Size: 500 to 1000 angstroms (50 to 100nm)
  • Maximum Linear Velocity: 3000cm per hour
  • Maximum Pressure: 100psi (6.9 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

Related Resources:

Review of antibody purification methods

Related Products:

Other Protein G Resins
All Antibody Purification Products


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
53125 Protein G UltraLink Resin
Formulation: Protein G on UltraLink Resin, slurried in water with sodium azide
Sufficient For: Binding 20mg human IgG/mL resin
2mL Product Instructions for product #53125 Protein G UltraLink Resin MSDS for product #53125 Protein G UltraLink Resin Certificate of Analysis for product #53125 Protein G UltraLink Resin $295.00

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53126 Protein G UltraLink Resin
Formulation: Protein G on UltraLink Resin, slurried in water with sodium azide
Sufficient For: Binding 20mg human IgG/mL resin
10mL Product Instructions for product #53126 Protein G UltraLink Resin MSDS for product #53126 Protein G UltraLink Resin Certificate of Analysis for product #53126 Protein G UltraLink Resin $990.00

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53127 Protein G UltraLink Columns, 2mL
Formulation: 5mL-capacity columns containing 2mL resin
Sufficient For: Binding 40mg human IgG per column
2 columns Product Instructions for product #53127 Protein G UltraLink Columns, 2mL MSDS for product #53127 Protein G UltraLink Columns, 2mL Certificate of Analysis for product #53127 Protein G UltraLink Columns, 2mL $563.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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