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Protein A UltraLink Resin


Protein A beads based on a durable resin for medium pressure affinity purification of IgG.

Pierce Protein A UltraLink Resin

Thermo Scientific Pierce Protein A UltraLink Resin is an extremely durable beaded polyacrylamide support to which IgG-binding Protein A has been immobilized for use in IgG purification methods.

The Protein A UltraLink Resin is used to bind and purify IgG in gravity-flow, spin-column or medium-pressure chromatography methods. Protein A is a popular antibody-binding protein that is especially useful for affinity purification of IgG from rabbit or human serum.

Highlights:

  • Immobilized Protein A – specifically binds IgG from several mammalian species, especially most subtypes of human and rabbit IgG
  • Standard capacity – loading of immobilized Protein A provides a binding capacity of approx. 16mg human IgG per milliliter of resin
  • UltraLink Resin – acrylamide-based support provides an alternative to crosslinked beaded agarose supports for medium pressure applications

Product Details:

Protein A is a cell wall component produced by several strains of Staphylococcus aureus. The 46.7kDa-protein consists of a single polypeptide chain that is essentially devoid of carbohydrate. Native Protein A has contains four high-affinity (Ka = 10^8/mol) binding sites that are capable of interacting with the Fc region of IgG-class antibodies from selected mammalian species. IgG-binding function is optimal at pH 8.2, but efficient binding also occurs in neutral and physiological buffers (pH 7.0 to 7.6).

UltraLink Resin is an azlactone-activated polyacrylamide support that is hydrophilic, charge-free, high capacity, highly crosslinked, rigid and porous. This support is especially useful for medium pressure techniques when using large sample volumes requiring fast-flow techniques (FPLC) and large-scale applications. Unlike typical beaded agarose supports, UltraLink Support does not crush or collapse when subjected to high flow rates that result in pressures exceeding 25psi.

Properties of Thermo Scientific UltraLink Resin:

  • Support pH Stability: 1 to 13
  • Average Particle Size: 50 to 80 microns
  • Exclusion Limit: greater than 2,000,000 daltons
  • Average Surface Area: greater than 250m2 per gram of beads
  • Average Pore Volume: greater than 1.2mL per gram of beads (> 60% of bead volume)
  • Pore Size: 500 to 1000 angstroms (50 to 100nm)
  • Maximum Linear Velocity: 3000cm per hour
  • Maximum Pressure: 100psi (6.9 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

Related Resources:

Review of antibody purification methods

Related Products:

All Protein A Resins (including Protein A Agarose and IgG Purification Kits)
All Antibody Purification Products


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
53139 Protein A UltraLink Resin
Formulation: Protein A on UltraLink Resin, slurried in water with sodium azide
Sufficient For: Binding 16mg human IgG/mL resin
5mL Product Instructions for product #53139 Protein A UltraLink Resin MSDS for product #53139 Protein A UltraLink Resin Certificate of Analysis for product #53139 Protein A UltraLink Resin $380.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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