Update profile
    Change password
    Sign out

Pierce Protein A Magnetic Beads


High-performance magnetic affinity particles for antibody purification and IP assays.

Pierce Protein A Magnetic Beads

Thermo Scientific Pierce Protein A Magnetic Beads are high-capacity and high-throughput affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.

Recombinant protein A, covalently attached to a blocked magnetic bead surface, can bind to antibodies from many different species, enabling purification of antibodies from crude extracts. Immunoprecipitation assays performed with protein A coated beads result in high yield of target antigen with very low background. Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in IP or Co-IP experiments. The Pierce Protein A Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific KingFisher Instruments, for high-throughput workflows.

Highlights:

  • High efficiency – equivalent or higher yield of IP target antigens than magnetic beads from other suppliers
  • Low non-specific binding – stable, pre-blocked beads provide highly purified product (e.g., antigen eluted in IP with antibody is devoid of contaminating protein from complex cell lysate)
  • Consistent – magnetic beads eliminate resin loss and provide for more efficient separation than traditional IP methods that use only centrifugation
  • Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

Applications:

  • IP and Co-IP experiments
  • Antibody purification

Product Details:

Thermo Scientific Pierce Protein A Magnetic Beads are used for purifying antibody from serum, cell culture supernatant or ascites, as well as for IP/Co-IP of antigens from cell or tissue extracts. Protein A can bind to antibodies from many different species, including mouse, human, rabbit, pig, dog, and cat. The protocol for the Pierce Protein A beads has been optimized to allow for high recovery and high purity of the isolated antibody or antigen. Antibody or antigen/antibody complex (IP) is first captured on the magnetic beads. The beads are washed and then the target is eluted with low pH elution buffer. IP performance is equivalent to or better than Protein A magnetic beads from other suppliers.

Characteristics of Thermo Scientific Pierce Protein A Magnetic Beads.
Composition: Recombinant Protein A monolayer covalently coupled to the surface of blocked magnetic beads
Magnetization: Superparamagnetic (no magnetic memory)
Mean Diameter: 1μm (nominal)
Density: 2.0g/cm3
Bead Concentration: 10mg/mL
Binding Capacity: ≥40μg of rabbit IgG/mg of beads;
≥400μg rabbit IgG/mL of beads

 

Binding capacity comparison of Protein A magnetic beads
Comparison of binding capacities for human and rabbit IgG. Thermo Scientific Pierce Protein A Magnetic Beads have approximately twice the IgG binding capacity of Dynabeads™ A (Life Technologies). Beads were added to a 96 deep-well plate (100μL beads per well). Using the Thermo Scientific KingFisher Flex Instrument, the beads were incubated for 1 hour with 400μg purified human or rabbit IgG. Binding was calculated using the Thermo Scientific BCA Protein Assay by subtracting the amount of IgG in the flow-throughs from the IgG loaded onto the beads.

 

Immunoprecipitation performance comparison of Protein A magnetic beads
Better immunoprecipitation results with Thermo Scientific Pierce Protein A Magnetic Beads. PP2A antibody (5μg) was incubated overnight at 4°C with 0.5mg of A549 cell lysate. Using the KingFisher Flex Instrument, 50μL each of Pierce Protein A Magnetic Beads, Dynabeads™ A (Life Technologies, Supplier 1), Protein A Magnetic Sepharose™ (GE Life Sciences; Supplier 2) and PureProteome™ Protein A beads (Millipore; Supplier 3) were added to 96 deep-well plates. The beads were incubated for 1 hour with the antigen/antibody complex at room temperature, washed twice in phosphate buffered saline containing 0.05% Tween™ 20, washed once in water and then eluted in 0.1M glycine, pH 2.0. Samples were resolved by SDS-PAGE and analyzed by Western blot for PP2A. The Pierce beads were found to have higher yield of PP2A than other Protein A beads tested. An accompanying silver-stained gel (not shown) confirmed that non-specific binding was negligible for all beads tested.

Related Resources:

Review of antibody purification methods
Compare Protein A, Protein G and other antibody binding proteins
Affinities of Proteins A, G and L by species and antibody type (Tech Tip #34)

Related Products:

Pierce Protein A/G Magnetic Beads
Other Pierce Magnetic Beads
Pierce Protein A Agarose
Thermo Scientific KingFisher Magnetic Particle Processors
Cell Lysis Reagents


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
88845 Protein A Magnetic Beads
Formulation: Recombinant Protein A monolayer covalently coupled to blocked superparamagnetic beads; supplied at 10mg/mL in PBS containing 0.05% Tween-20 and 0.05% NaN3
Sufficient For: Binding at least 400µg rabbit IgG/mL of bead
1mL Product Instructions for product #88845 Protein A Magnetic Beads MSDS for product #88845 Protein A Magnetic Beads Certificate of Analysis for product #88845 Protein A Magnetic Beads $159.00

Add to cart
88846 Protein A Magnetic Beads
Formulation: Recombinant Protein A monolayer covalently coupled to blocked superparamagnetic beads; supplied at 10mg/mL in PBS containing 0.05% Tween-20 and 0.05% NaN3
Sufficient For: Binding at least 400µg rabbit IgG/mL of bead
5mL Product Instructions for product #88846 Protein A Magnetic Beads MSDS for product #88846 Protein A Magnetic Beads Certificate of Analysis for product #88846 Protein A Magnetic Beads $605.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Share This Page
 
 

Follow Us
  Email Sign-up  Email Announcements

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747