Thermo Scientific™

Pierce™ Protein A Magnetic Beads

Catalog number: 88845
Thermo Scientific™

Pierce™ Protein A Magnetic Beads

Catalog number: 88845
Catalog Number
88845
Unit Size
1 mL
Price (USD)
Price: 201.00
Online price: 187.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
5 mL
Price (USD)
Price: 850.00
Online price: 804.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
888451 mL
Price: 201.00
Online price: 187.65
Your price:
-
888465 mL
Price: 850.00
Online price: 804.65
Your price:
-
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Thermo Scientific Pierce Protein A Magnetic Beads are high-capacity and high-throughput affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.

Features of Protein A Magnetic Beads:

High efficiency – equivalent or higher yield of IP target antigens than magnetic beads from other suppliers
Low non-specific binding – stable, pre-blocked beads provide highly purified product (e.g., antigen eluted in IP with antibody is devoid of contaminating protein from complex cell lysate)
Consistent – magnetic beads eliminate resin loss and provide for more efficient separation than traditional IP methods that use only centrifugation
Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

Recombinant protein A, covalently attached to a blocked magnetic bead surface, can bind to antibodies from many different species, enabling purification of antibodies from crude extracts. Immunoprecipitation assays performed with protein A coated beads result in high yield of target antigen with very low background. Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in IP or Co-IP experiments. The Pierce Protein A Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific KingFisher Instruments, for high-throughput workflows.

Applications:
• IP and Co-IP experiments
• Antibody purification

Thermo Scientific Pierce Protein A Magnetic Beads are used for purifying antibody from serum, cell culture supernatant or ascites, as well as for IP/Co-IP of antigens from cell or tissue extracts. Protein A can bind to antibodies from many different species, including mouse, human, rabbit, pig, dog, and cat. The protocol for the Pierce Protein A beads has been optimized to allow for high recovery and high purity of the isolated antibody or antigen. Antibody or antigen/antibody complex (IP) is first captured on the magnetic beads. The beads are washed and then the target is eluted with low pH elution buffer. IP performance is equivalent to or better than Protein A magnetic beads from other suppliers.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form
Liquid
Product Line
Pierce™
Quantity
1 mL
Protein Form
Recombinant
Target
Antibody
Type
Magnetic Affinity Bead
Ligand Type
Protein A

Contents & Storage

Upon receipt store at 4°C. Product shipped with an ice pack.

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