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Protein A High-Capacity Agarose and Kits


Premium Protein A beads, columns and kits for human and rabbit IgG antibody purification.

Pierce Protein A Agarose IgG Purification Kits

Thermo Scientific Pierce Protein A Plus Agarose is a versatile, high-performance affinity resin for antibody purification that is available as bottled agarose beads, pre-packed spin columns, 96-well filter plates and complete IgG purification kits.

Pierce Protein A Plus Agarose consists of purified native Protein A that has been covalently immobilized at high density onto high-quality crosslinked 6% beaded agarose (CL-6B). Among the many available varieties of immobilized Protein A affinity resins, this one provides the most versatile combination of chromatographic features for high yield and high purity purification of whole IgG from mammalian serum samples. The agarose beads have physical and chemical properties suitable for many affinity purification systems. Accordingly, Pierce Protein A Plus Agarose is offered in several convenient package formats, including bottled resin slurries, three sizes of spin columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates.

Highlights:

  • Native Protein A – immobilized Protein A is ideal for polyclonal IgG purification from human, rabbit, pig, dog or cat serum
  • Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
  • Inert and stable – superior manufacturing method immobilizes Protein A by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
  • High capacity – this “Plus” variety of Pierce Protein A Agarose has a dense load of immobilized Protein A, providing a binding capacity greater than 34mg human IgG/mL resin (approx. 16 to 17mg mouse IgG/mL resin)
  • Multiple formats – choose from bottled resin slurries, centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates

Includes:

  • Purification kits contain Protein A Plus Spin Columns, binding buffer, elution buffer and neutralization buffer

Product Details:

Protein A is a cell wall component produced by several strains of Staphylococcus aureus. The 46.7kDa-protein consists of a single polypeptide chain that is essentially devoid of carbohydrate. Native Protein A has contains four high-affinity (Ka = 10^8/mol) binding sites that are capable of interacting with the Fc region of IgG-class antibodies from selected mammalian species. IgG-binding function is optimal at pH 8.2, but efficient binding also occurs in neutral and physiological buffers (pH 7.0 to 7.6).

Protein A is a bacteria-derived protein that binds with high affinity and specificity to the Fc portion of antibodies, especially those of the IgG class. Compared to its alternative (Protein G), Protein A provides the highest binding capacity for subclasses of IgG from rabbits, pigs, dogs and cats. Protein A also has higher binding capacity for human IgG, except for IgG3, which it binds weakly. Protein A binds weakly to mouse IgG1 and is not recommended for most applications with that antibody isotype.

Pierce Protein A Plus Agarose is prepared using Thermo Scientific AminoLink Coupling Chemistry, which provides several advantages compared to traditional methods of ligand immobilization. AminoLink Immobilization results in conjugation between sugar monomers of the agarose beads and native lysine residues on the Protein A via simple amide bonds. Unlike typical cyanogen bromide (CNBr) immobilization, the AminoLink Method does not introduce any novel chemical groups that could cause undesired nonspecific binding and produces a stable, essentially irreversible bond. The result is a high-binding-capacity resin that retains functional immobilized Protein A through multiple rounds of antibody purification.

More Product Data

Properties of crosslinked 6% beaded agarose (CL-6B):

  • Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
  • Average Particle Size: 45 to 165 microns
  • Exclusion Limit: 10,000 to 4,000,000 daltons
  • Maximum Volumetric Flow Rate: approx. 1mL/minute (for 1cm diameter column)
  • Maximum Linear Velocity: 30cm per hour
  • Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

References:

  1. Sjoquist, J., et al. (1972) Eur. J. Biochem.29, 572-578.
  2. Hjelm, H., et al. (1975) Eur. J. Biochem.57, 395-403.
  3. Sjoholm, I., et al. (1975) Eur. J. Biochem.51, 55-6.
  4. Wang, B., et al. (1999) Proc. Nat. Acad. Sci. USA.96, 1627-1632.
  5. Higashi, I. , et al. (2000) Clin. Chem. 46, 297-299.

Related Resources:

Review of antibody purification methods
Compare Protein A, Protein G and other antibody binding proteins
Protein A, Protein G, Protein L affinity by species and antibody type (Tech Tip #34)

Related Products:

Other Protein A Resins
All Antibody Purification Products
Pierce Protein A/G Magnetic Beads
MagnaBind Protein A Magnetic Beads
Immunoprecipitation Kits

Available Formats:

  • Resin Slurries – 1mL, 5mL, 25mL bottles
  • Spin Columns – 0.2mL (microcentrifuge), 1mL, 5mL columns
  • Purification Kits – complete kits in all three column sizes
  • Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
  • 96-well Spin Plates – pre-dispensed 96-well filter plates, compatible with centrifugation and vacuum manifold systems for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations. (see Protein A 96-well Spin Plate data)
  • Bulk and Custom – let us know your needs for bulk Pierce Protein A Plus Agarose

Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
89924 Pierce Protein A Chromatography Cartridges, 1mL
Formulation: 1mL resin cartridges with LC and Luer-Lok Fittings
Sufficient For: Binding 35mg human IgG per cartridge

Learn more about Pierce Chromatography Cartridges.
2 cartridges Product Instructions for product #89924 Pierce Protein A Chromatography Cartridges, 1mL MSDS for product #89924 Pierce Protein A Chromatography Cartridges, 1mL Certificate of Analysis for product #89924 Pierce Protein A Chromatography Cartridges, 1mL $177.00

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89925 Pierce Protein A Chromatography Cartridges, 5mL
Formulation: 5mL resin cartridges with LC and Luer-Lok Fittings
Sufficient For: Binding 175mg human IgG per cartridge

Learn more about Pierce Chromatography Cartridges.
1 cartridge Product Instructions for product #89925 Pierce Protein A Chromatography Cartridges, 5mL MSDS for product #89925 Pierce Protein A Chromatography Cartridges, 5mL Certificate of Analysis for product #89925 Pierce Protein A Chromatography Cartridges, 5mL $350.00

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22810 Pierce Protein A Plus Agarose
Formulation: Protein A on crosslinked 6% beaded agarose, slurried in water with sodium azide
Sufficient For: Binding ≥34mg human IgG/mL resin; 16 to 17mg mouse IgG/mL resin
1mL Product Instructions for product #22810 Pierce Protein A Plus Agarose MSDS for product #22810 Pierce Protein A Plus Agarose Certificate of Analysis for product #22810 Pierce Protein A Plus Agarose $105.00

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22811 Pierce Protein A Plus Agarose
Formulation: Protein A on crosslinked 6% beaded agarose, slurried in water with sodium azide
Sufficient For: Binding ≥34mg human IgG/mL resin; 16 to 17mg mouse IgG/mL resin
5mL Product Instructions for product #22811 Pierce Protein A Plus Agarose MSDS for product #22811 Pierce Protein A Plus Agarose Certificate of Analysis for product #22811 Pierce Protein A Plus Agarose $417.00

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22812 Pierce Protein A Plus Agarose
Formulation: Protein A on crosslinked 6% beaded agarose, slurried in water with sodium azide
Sufficient For: Binding ≥34mg human IgG/mL resin; 16 to 17mg mouse IgG/mL resin
25mL Product Instructions for product #22812 Pierce Protein A Plus Agarose MSDS for product #22812 Pierce Protein A Plus Agarose Certificate of Analysis for product #22812 Pierce Protein A Plus Agarose $1,460.00

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22812B Pierce Protein A Plus Agarose
Support: Crosslinked 6% beaded agarose
Capacity: > 34mg human IgG/mL of resin; 16 to 17mg mouse IgG/mL of resin
Custom MSDS for product #22812B Pierce Protein A Plus Agarose Certificate of Analysis for product #22812B Pierce Protein A Plus Agarose
89952 NAb Protein A Plus Spin Columns, 0.2mL
Formulation: 0.8mL-capacity microcentrifuge columns containing 0.2mL resin
Sufficient For: Binding 7mg human IgG per column
10 columns Product Instructions for product #89952 NAb Protein A Plus Spin Columns, 0.2mL MSDS for product #89952 NAb Protein A Plus Spin Columns, 0.2mL Certificate of Analysis for product #89952 NAb Protein A Plus Spin Columns, 0.2mL $245.00

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89956 NAb Protein A Plus Spin Columns, 1mL
Formulation: 5mL-capacity centrifuge columns containing 1mL resin
Sufficient For: Binding 35mg human IgG per column
5 columns Product Instructions for product #89956 NAb Protein A Plus Spin Columns, 1mL MSDS for product #89956 NAb Protein A Plus Spin Columns, 1mL Certificate of Analysis for product #89956 NAb Protein A Plus Spin Columns, 1mL $458.00

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89960 NAb Protein A Plus Spin Column, 5mL
Formulation: 22mL-capacity centrifuge columns containing 5mL resin
Sufficient For: Binding 175mg human IgG per column
1 column Product Instructions for product #89960 NAb Protein A Plus Spin Column, 5mL MSDS for product #89960 NAb Protein A Plus Spin Column, 5mL Certificate of Analysis for product #89960 NAb Protein A Plus Spin Column, 5mL $438.00

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89948 NAb Protein A Plus Spin Kit, 0.2mL
Formulation: Multi-component kit
Sufficient For: Purifying approx. 7mg of human IgG per column

Kit Contents:
NAb Protein A Plus Spin Columns, 0.2mL, 10 columns
Microcentrifuge Tubes, 2mL, 80 tubes
PBS Pack (makes 500mL), 1 pack
IgG Elution Buffer (pH 2.8), 50mL
Neutralization Buffer, 12mL
10-column kit Product Instructions for product #89948 NAb Protein A Plus Spin Kit, 0.2mL MSDS for product #89948 NAb Protein A Plus Spin Kit, 0.2mL Certificate of Analysis for product #89948 NAb Protein A Plus Spin Kit, 0.2mL $260.00

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89978 NAb Protein A Plus Spin Kit, 1mL
Formulation: Multi-component kit
Sufficient For: Purifying approx. 35mg of human IgG per column

Kit Contents:
NAb Protein A Plus Spin Columns, 1mL, 2 columns
PBS Pack (makes 500mL), 1 pack
IgG Elution Buffer (pH 2.8), 240mL
Neutralization Buffer, 12mL
2-column kit Product Instructions for product #89978 NAb Protein A Plus Spin Kit, 1mL MSDS for product #89978 NAb Protein A Plus Spin Kit, 1mL Certificate of Analysis for product #89978 NAb Protein A Plus Spin Kit, 1mL $208.00

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45202 Protein A Spin Plate for IgG Screening
Formulation: 96-well filter plate containing 50µL Protein A Plus Agarose per well; Includes 3 collection plates
Sufficient For: Binding approx. 0.6mg human IgG or 0.3mg of mouse IgG per well

Learn more about Pierce 96-well Filter Spin Plates.
1 plate Product Instructions for product #45202 Protein A Spin Plate for IgG Screening MSDS for product #45202 Protein A Spin Plate for IgG Screening Certificate of Analysis for product #45202 Protein A Spin Plate for IgG Screening $235.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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