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Protein A, Protein G, Protein A/G and Protein L Coated Plates


Specifically capture antibodies and antibody complexes via Fc domains.

Pierce Protein A, G, A/G and L Coated Plates

Thermo Scientific Pierce Protein A, G, A/G and L Coated Plates provide alternatives to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

These Protein A, G, A/G and L Coated Plates are uniformly and stably coated with one of four popular immunoglobilin-binding proteins (Protein A, Protein G, Protein A/G or Protein L). They enable antibodies or pre-estabilished antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Highlights:

  • Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
  • Orient antibodies for maximum antigen-binding capacity
  • Immobilize antibodies for plate assays without prior purification
  • Ensure minimal variation (<5% well-to-well) from consistent coating
  • Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
  • Bind 2 to 5pmol of antibody per well (based on tests with rabbit IgG)

Protein A/G Coated Microplates:

  • Fusion protein containing four antibody-binding sites from Protein A and two from Protein G
  • Binds to most IgG species because it combines the specificities of Protein A and Protein G
  • Binds to Fc region of antibodies, ensuring optimal orientation
  • Binds well using a wide range of pH

Protein A Coated Microplates:

  • Binds strongly to IgG from human, rabbit, guinea pig, pig, dog and rhesus monkey
  • Binds strongly to mouse IgG2a, IgG2b and IgG3
  • Binds to Fc region of antibodies for optimal orientation

Protein G Coated Microplates:

  • Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat
  • Binds only to IgG – no cross-reactivity with other antibody classes
  • Binds to Fc region of antibodies for optimal orientation

Protein L Coated Microplates:

  • Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD)
  • Binds to the VL region of kappa light chains (human I, III, IV and Mouse I) without interfering with antigen-binding sites
  • Binds to Fab fragments and ScFv
  • Does not bind bovine, goat or sheep immunoglobulin
  • Binds weakly to rabbit immunoglobulin
  • Pre-blocked to reduce nonspecific binding

Product Details:

Bind more antibody using Protein A/G Coated Plates Greater signal compared to directly coated antibody. Comparison of signal obtained by antibody bound to Protein A and Protein G coated plates, and antibody passively bound to polystyrene plates. Goat Anti-Biotin was serially diluted across Protein A, Protein G and polystyrene plates. The Goat Anti-Biotin was detected by the addition of biotin, followed by streptavidin-HRP and finally TMB substrate.

Protein A References:

  1. Lawrenson, I.D., et al. (2002). J. Cell Sci. 115, 1059-1072.
  2. Asthagiri, A.R., et al. (1999). J. Biol. Chem. 274, 27119-27127.

Protein G References:

  1. Rauch, J., et al. (2003). J. Biol. Chem. 278, 47508-47515.
  2. Lai, Z., et al. (2002). Proc. Natl. Acad. Sci. USA 99, 14734-14739.

Protein L References:

  1. Björck, L. (1988). J. Immunol. 140, 1194-1197.
  2. Kastern, W., et al. (1992). J. Biol. Chem. 267, 12820-12825.
  3. Nilson, B.H.K., et al. (1993). J. Immunol. Methods 164, 33-40.
  4. Åkerström, B. and Björck, L. (1989). J. Biol. Chem. 264, 19740-19746.
  5. Rhee, J., et al. (2001). J. Biol. Chem. 276, 6640-6644.

Related Resources:

Assay development and ELISA technical guide
Comparison of Protein A, G, A/G and L immunoglobulin binding proteins

Related Products:

Secondary Antibody Coated Plates
Pierce 96-Well Microplates and Accessories


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
15130 Protein A Coated Plates, Clear, 96-Well
Formulation: Clear polystyrene 96-well plates coated at 100μL/well with Protein A and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 4pmol rabbit IgG per well

5 plates Product Instructions for product #15130 Protein A Coated Plates, Clear, 96-Well MSDS for product #15130 Protein A Coated Plates, Clear, 96-Well Certificate of Analysis for product #15130 Protein A Coated Plates, Clear, 96-Well $172.00

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15132 Protein A Coated Plates, Clear, 8-Well Strip
Formulation: Clear polystyrene 96-well strip plates coated at 100μL/well with Protein A and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 4pmol rabbit IgG per well

5 plates Product Instructions for product #15132 Protein A Coated Plates, Clear, 8-Well Strip MSDS for product #15132 Protein A Coated Plates, Clear, 8-Well Strip Certificate of Analysis for product #15132 Protein A Coated Plates, Clear, 8-Well Strip $192.00

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15154 Protein A Coated Plates, White, 96-Well
Formulation: White polystyrene 96-well plates coated at 100μL/well with Protein A and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 4pmol rabbit IgG per well
5 plates Product Instructions for product #15154 Protein A Coated Plates, White, 96-Well MSDS for product #15154 Protein A Coated Plates, White, 96-Well Certificate of Analysis for product #15154 Protein A Coated Plates, White, 96-Well $192.00

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15155 Protein A Coated Plates, Black, 96-Well
Formulation: Black polystyrene 96-well plates coated at 100μL/well with Protein A and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 4pmol rabbit IgG per well
5 plates Product Instructions for product #15155 Protein A Coated Plates, Black, 96-Well MSDS for product #15155 Protein A Coated Plates, Black, 96-Well Certificate of Analysis for product #15155 Protein A Coated Plates, Black, 96-Well $192.00

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15131 Protein G Coated Plates, Clear, 96-Well
Formulation: Clear polystyrene 96-well plates coated at 100μL/well with Protein G and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 2pmol rabbit IgG per well

5 plates Product Instructions for product #15131 Protein G Coated Plates, Clear, 96-Well MSDS for product #15131 Protein G Coated Plates, Clear, 96-Well Certificate of Analysis for product #15131 Protein G Coated Plates, Clear, 96-Well $192.00

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15133 Protein G Coated Plates, Clear, 8-Well Strip
Formulation: Clear polystyrene 96-well strip plates coated at 100μL/well with Protein G and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 2pmol rabbit IgG per well

5 plates Product Instructions for product #15133 Protein G Coated Plates, Clear, 8-Well Strip MSDS for product #15133 Protein G Coated Plates, Clear, 8-Well Strip Certificate of Analysis for product #15133 Protein G Coated Plates, Clear, 8-Well Strip $209.00

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15156 Protein G Coated Plates, White, 96-Well
Formulation: White polystyrene 96-well plates coated at 100μL/well with Protein G and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 2pmol rabbit IgG per well
5 plates Product Instructions for product #15156 Protein G Coated Plates, White, 96-Well MSDS for product #15156 Protein G Coated Plates, White, 96-Well Certificate of Analysis for product #15156 Protein G Coated Plates, White, 96-Well $209.00

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15157 Protein G Coated Plates, Black, 96-Well
Formulation: Black polystyrene 96-well plates coated at 100μL/well with Protein G and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 2pmol rabbit IgG per well
5 plates Product Instructions for product #15157 Protein G Coated Plates, Black, 96-Well MSDS for product #15157 Protein G Coated Plates, Black, 96-Well Certificate of Analysis for product #15157 Protein G Coated Plates, Black, 96-Well $209.00

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15138 Protein A/G Coated Plates, Clear, 8-Well Strip
Formulation: Clear polystyrene 96-well strip plates coated at 100μL/well with Protein A/G and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 5pmol rabbit IgG per well
5 plates Product Instructions for product #15138 Protein A/G Coated Plates, Clear, 8-Well Strip MSDS for product #15138 Protein A/G Coated Plates, Clear, 8-Well Strip Certificate of Analysis for product #15138 Protein A/G Coated Plates, Clear, 8-Well Strip $210.00

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15190 Protein L Coated Plates, Clear, 96-Well
Formulation: Clear polystyrene 96-well plates coated at 100μL/well with Protein L and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: (Binding capacity not determined)

5 plates Product Instructions for product #15190 Protein L Coated Plates, Clear, 96-Well MSDS for product #15190 Protein L Coated Plates, Clear, 96-Well Certificate of Analysis for product #15190 Protein L Coated Plates, Clear, 96-Well $205.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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