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Prostaglandin E2 ELISA Kit


Microplate competitive sandwich ELISA kit for prostaglandin E2 (PGE2).

Thermo Scientific Prostaglandin E2 ELISA Kits

Assay PGE2 (prostaglandin E2) levels in biological samples, such as tissue culture media, plasma, saliva and urine, with our Thermo Scientific Prostaglandin E2 Competitive ELISA Kit.

This PGE2 competitive ELISA kit is an immunoassay for the quantitative determination of prostaglandin E2 (PGE2) in biological fluids. The assay is based on the competition between PGE2 in the standard or sample and alkaline phosphatase conjugated PGE2 (PGE2-AP) for a limited amount of PGE2 monoclonal antibody bound to an anti-mouse IgG precoated 96-well plate. As the concentration of PGE2 in the sample increases, the amount of PGE2-AP captured by the coating antibody decreases. Thus, there is an inverse relationship between optical density (OD) and the amount of analyte in the sample.

Highlights:

  • Target: prostaglandin E2 (PGE2)
  • Format: colorimetric ELISA (p-NPP substrate) in clear 96-well plate
  • Measurement: absorbance at 405nm minus absorbance at 570nm
  • Assay Range: 39 to 5000pg/mL
  • Sensitivity: < 15.9pg/mL
  • Precision: dependent upon sample type and level (see instructions)
  • Sample Types: cell culture supernatant, serum, plasma (EDTA), whole blood, saliva, urine
  • Total Assay Time: 3 hours
  • Sample Size: 100μL
  • Specificity: no significant cross-reaction with 13,14-dihydro-15-keto-PGF2a; 6,15-keto-13-dihydro-PGF1a; Thromboxane B2; 2-Arachidonoylglycerol; Anandamide; PGD2; Arachadonic acid. Less than 1% cross-reaction with PFG2a; 6-keto-PGF1a; PGA2; PGB1; Approx. 16% cross-reaction with PGE3; Approx. 70% cross-reaction with PGE1.

Includes:

Complete kit contains pre-coated antibody-plate, detection antibodies, diluent, wash buffer, standard, substrate, and stop solution.

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EHPGE2 Prostaglandin E2 Competitive ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Mouse IgG Precoated 96-well Plate, 1 each
Reagent Diluent, 30mL
PGE2 Antibody, 6mL
PGE2-AP Conjugate, 6mL
PGE2 Standard, 0.55mL
10X Wash Buffer, 30mL
Substrate Solution 20mL
Stop Solution, 6mL
Plate Sealer, 1 each
PGE2 Assay Layout Sheet, 1 each
1-plate kit MSDS for product #EHPGE2 Prostaglandin E2 Competitive ELISA Kit $350.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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