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Precise Tris-HEPES Gels, 10 x 8.5cm


45-minute run-time provides quick results.

Thermo Scientific Precise Protein Gels

Thermo Scientific Precise Protein Gels are precast polyacrylamide gels for protein electrophoresis (PAGE) that use Tris-HEPES running buffer, have long shelf lives and provide compatibility with different commercial gel tanks.

Precise Gels are cast in a durable plastic cassette using a neutral-pH buffer that prevents polyacrylamide breakdown and results in a long shelf life. High-resolution staining and transfer of proteins is accomplished quickly on these 1mm thick mini gels. Gels are individually packaged in an easy-to-open plastic pouch and are ready to run with no comb or tape to remove. Following electrophoresis, the cassettes are easy to dismantle (no special tools required) without damaging the gel. Precise Gels are available in gradient (4 to 20% and 8 to 16%) and fixed (8%, 10% and 12%) concentrations and in 10-well, 12-well and 15-well formats.

Highlights:

  • High-performance – unique running buffer produces excellent separation and high-resolution protein bands
  • Long shelf life – up to 12-month stability ensures consistent performance for the duration of each package of gels
  • Fast – 45-minute run-time provides results quickly; transfers efficiently to nitrocellulose and PVDF membranes in just 90 minutes; stains quickly coomassie and silver stains
  • Large-volume wells – innovative well design accommodates twice the sample volume of typical mini-gel cassette wells (10-well=50µL; 12-well=30µL; 15-well=25µL)
  • Compatible – cassettes fit many popular mini-gel tanks, and gels can be loaded using standard Laemmli sample loading buffers
  • Several convenient styles – choose from five gel densities and three well formats

Specifications:

Specifications of Precise Protein Gels (Tris-HEPES Gels).
  • Cassette dimensions: 10cm x 8.5cm x 4.5mm
  • Gel dimensions: 8cm x 5.8cm x 1mm
  • Storage conditions: up to 1 year at 4°C
  • Stacking gel: 4% polyacrylamide
  • Buffer system in gel: Tris-HCl, pH 7
  • SDS content: none
  • Required running buffer: Tris-HEPES-SDS (Part No. 28398)
  • Recommended sample buffer: Tris-HCl-SDS (any type)

Compatible gel tanks for Precise Tris-HEPES Gels.
  • Fisher Biotech™ Vertical Minigel System
  • BioRad™ Mini-PROTEAN™ II and 3
  • Life Technologies XCell I, II™ and Surelock™
  • IBI Universal Protein System; EC 4-Cell
  • Hoefer Tall Mighty Small™ (SE 280), Mighty Small II (SE 260/ SE 250), Mini VE System (SE 300)
  • C.B.S. Scientific MGV 302/402
  • Daiichi Mini 2-Gel and 6-Gel
  • GradiGel Mini 4-Cell

Product Details:

Resolution and migration profiles for  Thermo Scientific Precise Protein Gels.
Chart of resolution and migration profiles for Thermo Scientific Precise Protein Gels (Tris-HEPES Gels). Values in each lane are protein sizes in kilodaltons (kDa) for the tested protein molecular weight marker.

SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) is used to determine the approximate molecular weights of proteins with reference to the mobility of standard proteins on the same gel. Gradient gels provide the appropriate gel pore size, making molecular weight estimation more accurate by sharpening stained protein bands.

The most popular buffer system for SDS-PAGE is the Laemmli system [Nature 227, 680-686 (1970)]. This system, however, is limited by extended running times and gel instability from hydrolysis of polyacrylamide to acrylic acid in alkaline conditions. This chemical change alters gel conductivity, causing a constant change in protein migration patterns with time.

Polyacrylamide hydrolysis does not occur at neutral pH, and Thermo Scientific Precise Protein Gels are cast at pH 7, yielding a long shelf life and assured reproducibility of protein migration patterns. The extra stability of Precise Protein Gels is combined with a new Tris-HEPES running buffer to give speed and excellent resolution with the same size ranges as the Laemmli system. The buffer can be made according to the recipe provided in the instruction booklet or purchased premixed.

Related Resources:

Protein gel electrophoresis technical guide

Related Products:

Membranes and Filter Paper
Molecular Weight Markers and Sample Loading Buffer
BupH Tris-Glycine Buffer Packs – and other BupH Buffer Packs
10X and 20X Concentrated Electrophoresis Buffers


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
25200 Precise Protein Gels, 8%, 10-Well
Formulation: Precast 8% polyacrylamide gels for SDS-PAGE
Sufficient For: 10 samples per gel, 50μL capacity per well
10 gels Product Instructions for product #25200 Precise Protein Gels, 8%, 10-Well MSDS for product #25200 Precise Protein Gels, 8%, 10-Well Certificate of Analysis for product #25200 Precise Protein Gels, 8%, 10-Well $128.00

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25201 Precise Protein Gels, 10%, 10-Well
Formulation: Precast 10% polyacrylamide gels for SDS-PAGE
Sufficient For: 10 samples per gel, 50μL capacity per well
10 gels Product Instructions for product #25201 Precise Protein Gels, 10%, 10-Well MSDS for product #25201 Precise Protein Gels, 10%, 10-Well Certificate of Analysis for product #25201 Precise Protein Gels, 10%, 10-Well $128.00

Add to cart
25202 Precise Protein Gels, 12%, 10-Well
Formulation: Precast 12% polyacrylamide gels for SDS-PAGE
Sufficient For: 10 samples per gel, 50μL capacity per well
10 gels Product Instructions for product #25202 Precise Protein Gels, 12%, 10-Well MSDS for product #25202 Precise Protein Gels, 12%, 10-Well Certificate of Analysis for product #25202 Precise Protein Gels, 12%, 10-Well $128.00

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25203 Precise Protein Gels, 8-16%, 10-Well
Formulation: Precast 8 to 16% gradient polyacrylamide gels for SDS-PAGE
Sufficient For: 10 samples per gel, 50μL capacity per well
10 gels Product Instructions for product #25203 Precise Protein Gels, 8-16%, 10-Well MSDS for product #25203 Precise Protein Gels, 8-16%, 10-Well Certificate of Analysis for product #25203 Precise Protein Gels, 8-16%, 10-Well $128.00

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25204 Precise Protein Gels, 4-20%, 10-Well
Formulation: Precast 4 to 20% gradient polyacrylamide gels for SDS-PAGE
Sufficient For: 10 samples per gel, 50μL capacity per well
10 gels Product Instructions for product #25204 Precise Protein Gels, 4-20%, 10-Well MSDS for product #25204 Precise Protein Gels, 4-20%, 10-Well Certificate of Analysis for product #25204 Precise Protein Gels, 4-20%, 10-Well $128.00

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25220 Precise Protein Gels, 8%, 12-Well
Formulation: Precast 8% polyacrylamide gels for SDS-PAGE
Sufficient For: 12 samples per gel, 30μL capacity per well
10 gels Product Instructions for product #25220 Precise Protein Gels, 8%, 12-Well MSDS for product #25220 Precise Protein Gels, 8%, 12-Well Certificate of Analysis for product #25220 Precise Protein Gels, 8%, 12-Well $128.00

Add to cart
25221 Precise Protein Gels, 10%, 12-Well
Formulation: Precast 10% polyacrylamide gels for SDS-PAGE
Sufficient For: 12 samples per gel, 30μL capacity per well
10 gels Product Instructions for product #25221 Precise Protein Gels, 10%, 12-Well MSDS for product #25221 Precise Protein Gels, 10%, 12-Well Certificate of Analysis for product #25221 Precise Protein Gels, 10%, 12-Well $128.00

Add to cart
25222 Precise Protein Gels, 12%, 12-Well
Formulation: Precast 12% polyacrylamide gels for SDS-PAGE
Sufficient For: 12 samples per gel, 30μL capacity per well
10 gels Product Instructions for product #25222 Precise Protein Gels, 12%, 12-Well MSDS for product #25222 Precise Protein Gels, 12%, 12-Well Certificate of Analysis for product #25222 Precise Protein Gels, 12%, 12-Well $128.00

Add to cart
25223 Precise Protein Gels, 8-16%, 12-Well
Formulation: Precast 8 to 16% gradient polyacrylamide gels for SDS-PAGE
Sufficient For: 12 samples per gel, 30μL capacity per well
10 gels Product Instructions for product #25223 Precise Protein Gels, 8-16%, 12-Well MSDS for product #25223 Precise Protein Gels, 8-16%, 12-Well Certificate of Analysis for product #25223 Precise Protein Gels, 8-16%, 12-Well $128.00

Add to cart
25224 Precise Protein Gels, 4-20%, 12-Well
Formulation: Precast 4 to 20% gradient polyacrylamide gels for SDS-PAGE
Sufficient For: 12 samples per gel, 30μL capacity per well
10 gels Product Instructions for product #25224 Precise Protein Gels, 4-20%, 12-Well MSDS for product #25224 Precise Protein Gels, 4-20%, 12-Well Certificate of Analysis for product #25224 Precise Protein Gels, 4-20%, 12-Well $128.00

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25240 Precise Protein Gels, 8%, 15-Well
Formulation: Precast 8% polyacrylamide gels for SDS-PAGE
Sufficient For: 15 samples per gel, 25μL capacity per well
10 gels Product Instructions for product #25240 Precise Protein Gels, 8%, 15-Well MSDS for product #25240 Precise Protein Gels, 8%, 15-Well Certificate of Analysis for product #25240 Precise Protein Gels, 8%, 15-Well $128.00

Add to cart
25241 Precise Protein Gels, 10%, 15-Well
Formulation: Precast 10% polyacrylamide gels for SDS-PAGE
Sufficient For: 15 samples per gel, 25μL capacity per well
10 gels Product Instructions for product #25241 Precise Protein Gels, 10%, 15-Well MSDS for product #25241 Precise Protein Gels, 10%, 15-Well Certificate of Analysis for product #25241 Precise Protein Gels, 10%, 15-Well $128.00

Add to cart
25242 Precise Protein Gels, 12%, 15-Well
Formulation: Precast 12% polyacrylamide gels for SDS-PAGE
Sufficient For: 15 samples per gel, 25μL capacity per well
10 gels Product Instructions for product #25242 Precise Protein Gels, 12%, 15-Well MSDS for product #25242 Precise Protein Gels, 12%, 15-Well Certificate of Analysis for product #25242 Precise Protein Gels, 12%, 15-Well $128.00

Add to cart
25243 Precise Protein Gels, 8-16%, 15-Well
Formulation: Precast 8 to 16% gradient polyacrylamide gels for SDS-PAGE
Sufficient For: 15 samples per gel, 25μL capacity per well
10 gels Product Instructions for product #25243 Precise Protein Gels, 8-16%, 15-Well MSDS for product #25243 Precise Protein Gels, 8-16%, 15-Well Certificate of Analysis for product #25243 Precise Protein Gels, 8-16%, 15-Well $128.00

Add to cart
25244 Precise Protein Gels, 4-20%, 15-Well
Formulation: Precast 4 to 20% gradient polyacrylamide gels for SDS-PAGE
Sufficient For: 15 samples per gel, 25μL capacity per well
10 gels Product Instructions for product #25244 Precise Protein Gels, 4-20%, 15-Well MSDS for product #25244 Precise Protein Gels, 4-20%, 15-Well Certificate of Analysis for product #25244 Precise Protein Gels, 4-20%, 15-Well $128.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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