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Porcine TNF alpha ELISA Kits

Microplate kits and reagent sets for sandwich ELISA of pig TNF alpha.

Thermo Scientific Porcine TNF alpha ELISA Kits

Assay TNFa (porcine tumor necrosis factor alpha) levels in pig serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Porcine TNF alpha ELISA Kits.

These porcine TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.


  • Target: natural and recombinant porcine TNFα (porcine tumor necrosis factor alpha)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 31.3 to 2000pg/mL
  • Sensitivity: < 5pg/mL
  • Precision: dependent upon sample type and level (see instructions)
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 4 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12p70, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, Eotaxin, GCSF, GMCSF, GROα, GROβ, IFNγ, IFNα, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, MIP-1α, MIP-1β, TGFβ, TNFβ or RANTES; mouse TNFα; rat TNFα; bovine TNFα. This ELISA uses antibodies raised against human TNFα. This ELISA reacts with porcine and human TNFα at 100%.

Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit.

Video of the Thermo Scientific TNF alpha ELISA Kit


Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.

» Compare ELISA Kit formats »


Porcine TNF alpha ELISA standard curve
Porcine TNF alpha ELISA standard curve. An example standard curve for the Thermo Scientific Porcine TNF alpha ELISA Kit (Part No. EP2TNFA), with error bars based on four replicates for each tumor necrosis factor alpha standard concentration. Also see standard curve for the TNFα Reagent Kit.


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This product is for In Vitro experimental use only. Not for resale without express authorization.

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
EP2TNFA Porcine TNF alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-TNF alpha Precoated 96-well Plate, 1 each
Lyophilized Recombinant Porcine TNF alpha Standard, 2 vials
Sample Diluent, 14mL
30X Wash Buffer, 50mL
Biotinylated Antibody Reagent, 12mL
Streptavidin-HRP Reagent, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Sealers, 6 each
1-plate kit Product Instructions for product #EP2TNFA Porcine TNF alpha ELISA Kit MSDS for product #EP2TNFA Porcine TNF alpha ELISA Kit $399.00

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ESS0003 Porcine TNF alpha ELISA Reagent Kit
Formulation: Antibodies and complete reagent set for ELISA
Sufficient For: 5 x 96 microplate wells

Kit Contents:
TNF alpha Coating Antibody, 1 vial
TNF alpha Detection Antibody, 1 vial
Porcine TNF alpha Standard, 5 vials
Streptavidin-HRP Conjugate, 0.25mL
TMB Substrate Solution, 55mL
Stop Solution, 55mL
5-plate kit Product Instructions for product #ESS0003 Porcine TNF alpha ELISA Reagent Kit MSDS for product #ESS0003 Porcine TNF alpha ELISA Reagent Kit $375.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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