Thermo Scientific Pierce PMPI is a maleimide-and-isocyanate crosslinker for attaching compounds to sulfhydryl groups (cysteines) after conjugating the linker to hydroxyl groups on a molecule by urethane (carbamate) bonds.
Features of PMPI:
• Reactive groups:maleimide and isocyanate • Reactive towards: sulfhydryl and hydroxyl groups • Short (8.7A), sulfhydryl-to-hydroxyl crosslinker with an aromatic spacer arm (noncleavable) • Maleimide group reacts with sulfhydryl groups to form stable thioether linkages • Isocyanate reacts with non-aqueous hydroxyl groups to form carbamate linkage • Excellent tool for conjugating hydroxyl-containing compounds such as steroids and vitamins • Targets and reaction solutions must not contain primary amines
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Reactivity
Carboxyl-Sulfhydryl
Cleavable
No
Description
PMPI
Molecular Weight (g/mol)
307.1
PEGylated
No
Spacer Arm Length
8.7 Å
Cell Permeability
Not determined
Shipping Condition
Ambient
Product Line
Pierce™
Labeling Method
Chemical Labeling
Crosslinker Type
Heterobifunctional
Reactive Moiety
Maleimide, Isocyanate
Spacer
Short (<10 Å)
Form
Solid
Quantity
50 mg
Solubility
DMF, DMSO
Format
Standard
Water Soluble
No
Product Type
Crosslinker
Contents & Storage
Upon receipt store at 4°C protected from moisture.