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Conjugate hydroxyl compounds via an isocyanate reactive group.

Pierce PMPI

Thermo Scientific Pierce PMPI is a maleimide-and-isocyanate crosslinker for attaching compounds to sulfhydryl groups (cysteines) after conjugating the linker to hydroxyl groups on a molecule by urethane (carbamate) bonds.


  • Reactive groups: maleimide and isocyanate
  • Reactive towards: sulfhydryl and hydroxyl groups
  • Short (8.7A), sulfhydryl-to-hydroxyl crosslinker with an aromatic spacer arm (noncleavable)
  • Maleimide group reacts with sulfhydryl groups to form stable thioether linkages
  • Isocyanate reacts with non-aqueous hydroxyl groups to form carbamate linkage
  • Excellent tool for conjugating hydroxyl-containing compounds such as steroids and vitamins
  • Targets and reaction solutions must not contain primary amines

Product Details:

p-Maleimidophenyl isocyanate; PMPI
Chemical structure of PMPI crosslinking reagent.


  1. Annunziato, M.E., et al. (1993). p-Maleimidophenyl isocyanate: A novel heterobifunctional linker for hydroxyl to thiol coupling. Bioconjugate Chem. 4, 212-218.

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
28100 PMPI
Formulation: p-Maleimidophenyl isocyanate; solid
Sufficient For: 233mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #28100 PMPI MSDS for product #28100 PMPI Certificate of Analysis for product #28100 PMPI $161.00

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28100B PMPI
Formulation: p-Maleimidophenyl isocyanate; solid
Custom MSDS for product #28100B PMPI Certificate of Analysis for product #28100B PMPI Bulk Quote Request

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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