Update profile
    Change password
    Sign out

Pierce Western Blot Signal Enhancer


Detect low-abundance proteins without changing substrate or antibody dilutions.

Thermo Scientific Pierce Western Blot Signal Enhancer

Thermo Scientific Pierce Western Blot Signal Enhancer is a two-reagent system for conditioning protein blots after transfer to greatly enhance the effectiveness of primary antibodies and intensify the final detection signal in Western blot experiments.

The Pierce Western Blot Signal Enhancer membrane treatment procedure is very simple, takes only 15 minutes and can be added to nearly any existing Western blotting protocol. The result is an increase in the intensity of target protein bands on the Western blot or detection of target proteins at levels that were previously not possible. The product is effective for signal intensification with both chemiluminescent and chromogenic substrates, especially with nitrocellulose membranes.

Highlights:

  • Increases protein detection – most protein targets show a three- to 10-fold increase in signal intensity, enabling much less protein to be detected with the same substrate and method
  • Improves antibody binding – the membrane-treatment reagent exposes and conditions target proteins so that specific antibodies can bind more effectively
  • Works for nearly any protein – signal enhancement has been demonstrated with targets such as IL-6, p53, NFκB, BRCA1 and EGF
  • Effective with any substrate – enhances both chemiluminescent (ECL) and colorimetric detection for Western blots
  • Compatible with any membrane – enhances signal on nitrocellulose and PVDF membrane, regardless of pore size (enhancement is less pronounced with PVDF)
  • Fast, 15-minute protocol – optimized for a combination of simplicity, speed and signal enhancement for most proteins
  • Ready-to-use – no formulating or diluting necessary, and the reagents are stable for storage at room temperature

Product Details:

Protocol summary for Pierce Western Blot Signal Enhancer
Protocol summary for Thermo Scientific Pierce Western Blot Signal Enhancer.

 

Chemiluminescent signal enhancement with Pierce Western Blot Signal Enhancer Enhanced chemiluminescent substrate detection using Thermo Scientific Pierce Western Blot Signal Enhancer. Identical gels and nitrocellulose blots of IL-6 protein samples (250 to 2000pg per well) were probed and detected with identical reagents and conditions after no treatment (top) or Enhancer-treatment (bottom) of the membrane. Final detection was with SuperSignal West Pico Chemiluminescent Substrate and exposure to film for 5 minutes.

 

Chromogenic substrate enhancement with Pierce Western Blot Signal Enhancer Enhanced chromogenic substrate detection using Thermo Scientific Pierce Western Blot Signal Enhancer. Identical gels and nitrocellulose blots of IL-6 protein samples (0.1 to 2µg per well) were probed and detected with identical reagents and conditions after no treatment (top) or Enhancer-treatment (bottom) of the membrane. Final detection was with NBT-BCIP substrate.

References:

  1. Aupperlee, M., et al. (2005). Endocrinology 146, 3577-3588.
  2. Li, Y., et al. (2004). Blood. 104, 1137-1144.
  3. MacMillan, D., et al. (2005). J. Cell. Sci. 118, 5443-5451.

Related Products:

SuperSignal Western Blot Enhancer
SuperSignal West Pico Chemiluminescent Substrate
NBT-BCIP Substrate Solution


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
21050 Pierce Western Blot Signal Enhancer
Formulation: Proprietary clear, colorless solutions
Sufficient For: 10 mini blots or 800cm2 membrane


Kit Contents:
Enhancer Reagent 1, 250mL
Enhancer Reagent 2, 250mL
500mL kit Product Instructions for product #21050 Pierce Western Blot Signal Enhancer MSDS for product #21050 Pierce Western Blot Signal Enhancer Certificate of Analysis for product #21050 Pierce Western Blot Signal Enhancer $129.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Share This Page
 
 

Follow Us
  Email Sign-up  Email Announcements

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747