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Pierce Trypsin Protease, MS Grade


An economical alternative to Promega Trypsin Gold for proteomic mass spectrometry.

Pierce Trypsin Protease, MS Grade

Thermo Scientific Pierce Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

Pierce Trypsin Protease, MS Grade, is a serine protease derived from porcine pancreatic extracts. The enzyme is TPCK-treated to eliminate chymotryptic activity and methylated to improve stability during protein digestion. This mass spectrometry-grade, modified trypsin is packaged in both frozen liquid format (100µg at 1mg/mL) and lyophilized formats (convenient 5 x 20μg, 5 x 100μg, and 1mg fill sizes).

Highlights:

  • Exceptional selectivity – cleaves at the carboxyl side of lysine and arginine residues with greater than 95% specificity
  • High purity – no detectable chymotrypsin activity
  • Enhanced stability – chemically modified for reduced autolytic activity
  • Economical – available in larger fill sizes that are more cost effective
  • Flexible – available in ready-to-use liquid format and three sizes of lyophilized format

Applications:

  • In-gel digestion of proteins from 1-D or 2-D gels
  • In-solution tryptic digestion of proteins

Product Details:

Trypsin is a serine protease that specifically cleaves at the carboxyl side of lysine and arginine residues. The selectivity of this enzyme is critical for reproducible protein digestion and mass spectrometry-based protein identification. Since chymotrypsin co-purifies with trypsin derived from natural sources, Pierce Trypsin Protease has been treated with TPCK to eliminate chymotryptic activity, improving digestion specificity. Native trypsin is also subject to autolysis which can reduce enzyme stability and efficiency. To reduce autolytic degradation, Pierce Trypsin Protease is chemically modified by methylation, yielding a highly active and more stable form of the enzyme.

In addition to possessing high specific activity and being resistant to autolytic digestion, Pierce Trypsin Protease can tolerate commonly used partially denaturing conditions, such as 0.1% SDS, 1M urea and 10% acetonitrile. Pierce Trypsin Protease is most active in pH ranges pH 7 to 9 and can be reversibly inactivated at pH < 4. Both the liquid and lyophilized forms of the enzyme are stable for > 1 yearr when stored at -20°C.

Excellent digestion performance with Thermo Scientific Pierce Trypsin, MS Grade
Excellent digestion performance with Thermo Scientific Pierce Trypsin Protease, MS Grade. Base peak chromatograms of a five-protein mixture sample digested with Pierce Trypsin Protease (top two) and Promega™ Trypsin Gold (bottom two). Samples (10μg each) were mixed with trypsin at a 1:50 ratio in a 50mM TEAB buffer (pH ~8) and incubated at 37°C for 30 minutes or 3 hours. Digested sample peptides (0.5μg each) were separated using nanoflow high-pressure liquid chromatography for analysis by a Thermo Scientific Velos Pro mass spectrometer. Click image to see full size.

Comparison of the cleavage selectivity of spectrometry-grade trypsin products.
Comparison of the cleavage selectivity of spectrometry-grade trypsin products. Five-protein mixture samples (10μg) were digested with Thermo Scientific Pierce Trypsin Protease or Promega™ Trypsin Gold for 3 hours and analyzed by LC/MS using a Thermo Scientific Velos Pro mass spectrometer. Data was searched using Mascot with "no enzyme" digestion settings. Greater than 95% cleavage selectivity for lysine and arginine (K/R) was observed for Pierce Trypsin Protease.

Percent sequence coverage of selected proteins in a protein sample digest.
  Thermo Scientific 
Pierce Trypsin
Protease, MS Grade
Promega™ 
Trypsin Gold,
MS Grade
Protein 30 min 3 hours 30 min 3 hours
Glutamate dehydrogenase 49 51 54 55
Myoglobin 68 82 68 78
Lactoperoxidase 41 45 46 45
Transferrin 63 64 67 64

General References:

  1. Biringer, R.G., et al. (2006). Brief Func. Genom. and Prot. 5:144-153.
  2. Schlosser, A., et al. (2005). Anal. Chem. 77:5243-5250.
  3. Wu, S-L., et al. (2005). J. Proteome Res. 4:1155-1170.

Related Products:

In-Gel Tryptic Digestion Kit
In-Solution Tryptic Digestion and Guanidination Kit
Mass spectrometry reagents and kits
Detergent Removal Products
Mass Spec Sample Prep Kit for Cultured Cells


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
90057 Trypsin Protease, MS Grade
Formulation: Purified porcine trypsin, lyophilized
5 x 20μg Product Instructions for product #90057 Trypsin Protease, MS Grade MSDS for product #90057 Trypsin Protease, MS Grade Certificate of Analysis for product #90057 Trypsin Protease, MS Grade $56.00

Add to cart
90058 Trypsin Protease, MS Grade
Formulation: Purified porcine trypsin, lyophilized
5 x 100μg Product Instructions for product #90058 Trypsin Protease, MS Grade MSDS for product #90058 Trypsin Protease, MS Grade Certificate of Analysis for product #90058 Trypsin Protease, MS Grade $246.00

Add to cart
90059 Trypsin Protease, MS Grade
Formulation: Purified porcine trypsin, lyophilized
1mg Product Instructions for product #90059 Trypsin Protease, MS Grade MSDS for product #90059 Trypsin Protease, MS Grade Certificate of Analysis for product #90059 Trypsin Protease, MS Grade $475.00

Add to cart
90305 Trypsin Protease, MS Grade, Frozen
Formulation: Purified porcine trypsin, 1mg/mL frozen liquid

1 x 100µg Product Instructions for product #90305 Trypsin Protease, MS Grade, Frozen MSDS for product #90305 Trypsin Protease, MS Grade, Frozen Certificate of Analysis for product #90305 Trypsin Protease, MS Grade, Frozen $45.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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