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Pierce Recombinant Protein L and Conjugates


Binds immunglobulin kappa light chains of many species and antibody classes.

Pierce Recombinant Protein L

Use purified and conjugated forms of Thermo Scientific Pierce Recombinant Protein L to probe and detect mouse and human antibodies containing kappa light chains in Western blotting, ELISA, IHC and other immunoassay protocols.

Protein L is an immunoglobulin-binding protein that was originally derived from the bacteria Peptostreptococcus magnus, but is now produced recombinantly. Protein L has the unique ability to bind through kappa light chain interactions without interfering with an antibody's antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L will bind to all classes of Ig (IgG, IgM, IgA, IgE and IgD). Protein L will also bind single chain variable fragments (scFv) and Fab fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse.

Highlights:

  • Contains four immunoglobulin-binding domains per protein
  • Binds members of all classes of Ig (IgG, IgM, IgA, IgE and IgD) that contain kappa light chains
  • Binds single chain variable fragments (scFv) and Fab fragments that contain kappa light chains

Properties of Recombinant Protein L:

  • Source: E. coli
  • Molecular Weight: ~35,800 (Apparent MW by SDS-PAGE: 36,000)
  • Form: salt-free powder
  • A280 of 0.1% solution: ~0.80
  • Isoelectric point (pI): 4.8

General References:

  1. Bjorck, L. (1988). Protein L: A novel bacterial cell wall protein with affinity for Ig L chains. J. Immunol. 140(4):1194-1197.
  2. Kastern, W., et al. (1992). Structure of peptostreptococcal protein L and identification of a repeated immunoglobulin light chain-binding domain. J. Biol. Chem. 267(18):12820-5.
  3. Nilson, B.H., et al. (1993). Purification of antibodies using protein L binding framework structures in the light chain variable domain. J. Immunol. Methods 164(1):33-40.
  4. Bottomley, S.P., et al. (1995). Cloning, expression and purification of Ppl-1, a kappa-chain binding protein, based upon protein L from Peptostreptococcus magnus. Bioseparation 5(6):359-67.
  5. Murphy, J.P., et al. (1996). Amplified expression and large-scale purification of protein L. Bioseparation 6(2):107-13.

Related Resources:

Comparison of Protein A, G, A/G and L
Binding characteristics of Protein A, Protein G, Protein A/G and Protein L (Tech Tip #34)

Related Products:

Protein L Agarose Resins, Columns and Kits


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
21189 Recombinant Protein L
Formulation: Salt-free powder
1mg Product Instructions for product #21189 Recombinant Protein L MSDS for product #21189 Recombinant Protein L Certificate of Analysis for product #21189 Recombinant Protein L $135.00

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77679 Recombinant Protein L
Formulation: Salt-free powder

50mg Product Instructions for product #77679 Recombinant Protein L MSDS for product #77679 Recombinant Protein L Certificate of Analysis for product #77679 Recombinant Protein L $1,240.00

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77680 Recombinant Protein L
Formulation: Salt-free powder

500mg Product Instructions for product #77680 Recombinant Protein L MSDS for product #77680 Recombinant Protein L Certificate of Analysis for product #77680 Recombinant Protein L $4,960.00

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21189B Recombinant Protein L
Formulation: Salt-free powder
Custom MSDS for product #21189B Recombinant Protein L Certificate of Analysis for product #21189B Recombinant Protein L
29997 Recombinant Protein L, Biotinylated
Formulation: Lyophilized powder from protein in buffered solution
0.5mg Product Instructions for product #29997 Recombinant Protein L, Biotinylated MSDS for product #29997 Recombinant Protein L, Biotinylated Certificate of Analysis for product #29997 Recombinant Protein L, Biotinylated $189.00

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32420 Recombinant Protein L, Peroxidase Conjugated
Formulation: Lyophilized powder from protein in buffered solution
0.5mg MSDS for product #32420 Recombinant Protein L, Peroxidase Conjugated Certificate of Analysis for product #32420 Recombinant Protein L, Peroxidase Conjugated $171.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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