Use purified and conjugated forms of Thermo Scientific Pierce Recombinant Protein L to probe and detect mouse and human antibodies containing kappa light chains in Western blotting, ELISA, IHC and other immunoassay protocols.
Protein L is an immunoglobulin-binding protein that was originally derived from the bacteria Peptostreptococcus magnus, but is now produced recombinantly. Protein L has the unique ability to bind through kappa light chain interactions without interfering with an antibody's antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L will bind to all classes of Ig (IgG, IgM, IgA, IgE and IgD). Protein L will also bind single chain variable fragments (scFv) and Fab fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse.
- Contains four immunoglobulin-binding domains per protein
- Binds members of all classes of Ig (IgG, IgM, IgA, IgE and IgD) that contain kappa light chains
- Binds single chain variable fragments (scFv) and Fab fragments that contain kappa light chains
Properties of Recombinant Protein L:
- Source: E. coli
- Molecular Weight: ~35,800 (Apparent MW by SDS-PAGE: 36,000)
- Form: salt-free powder
- A280 of 0.1% solution: ~0.80
- Isoelectric point (pI): 4.8
- Bjorck, L. (1988). Protein L: A novel bacterial cell wall protein with affinity for Ig L chains. J. Immunol. 140(4):1194-1197.
- Kastern, W., et al. (1992). Structure of peptostreptococcal protein L and identification of a repeated immunoglobulin light chain-binding domain. J. Biol. Chem. 267(18):12820-5.
- Nilson, B.H., et al. (1993). Purification of antibodies using protein L binding framework structures in the light chain variable domain. J. Immunol. Methods 164(1):33-40.
- Bottomley, S.P., et al. (1995). Cloning, expression and purification of Ppl-1, a kappa-chain binding protein, based upon protein L from Peptostreptococcus magnus. Bioseparation 5(6):359-67.
- Murphy, J.P., et al. (1996). Amplified expression and large-scale purification of protein L. Bioseparation 6(2):107-13.
Comparison of Protein A, G, A/G and L
Binding characteristics of Protein A, Protein G, Protein A/G and Protein L (Tech Tip #34)
Protein L Agarose Resins, Columns and Kits