Thermo Scientific Pierce Protein Transfection Reagent is a cationic lipid mixture for complexation with proteins, peptides, antibodies and other biologically active molecules to allow their direct intracellular delivery.
This is a unique cationic lipid mixture (previously called Pro-Ject Reagent) which, when complexed with proteins or peptides, allows direct intracellular delivery of proteins, peptides, antibodies and other biologically active molecules. Reagent protein complexes attach to negatively charged cell surfaces and either can directly fuse with the membrane and deliver the captured protein into the cell or be endocytosed by the cell and then fuse with the endosome, releasing the captured protein into the cytoplasm. Since the complex formed is noncovalent it does not interfere with the protein's biological activity.
- No need to isolate, clone and transfect gene sequences —Saves days of work
- Optimum delivery in three to four hours after incubation —Fast protein delivery
- Delivery complex is non-covalent —Proteins and peptides retain biological activity after delivery
- Works with proteins, peptides and fluorescently labeled antibodies —Versatility
Molecules that have been successfully delivered with the transfection reagent include ß-galactosidase, fluorescent-antibody, green fluorescent protein (GFP), high and low molecular weight dextran sulfate, phycoerythrin-BSA, caspase 3, caspase 8 and granzyme B.
Protocol for Optimization of Protein Transfection.
Cell types successfully tested with the transfection reagent. Transfection efficiencies vary with cell type. For example, easy to transfect cell lines such as NIH-3T3 cells typically result in >90% transfection efficiencies. HeLa cells generally give 40-50% transfection efficiencies.
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TurboFect in vitro Transfection Reagent