Update profile
    Change password
    Sign out

Pierce Protein-Free Blocking Buffers


Block Western blots and ELISA plates without introducing exogenous proteins.

Pierce Protein-Free Blocking Buffers

Thermo Scientific Pierce Protein-Free Blocking Buffers are PBS and TBS formulations of a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background.

Traditional blocking buffers contain proteins that can cross-react with immunodetection systems, resulting in high background and reduced signal. Typical commercial alternatives to protein-based blocking agents are ineffective. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes and other surfaces for ELISA, Western blotting, glass slide arrays and other applications.

Highlights:

  • Protein-free blocker – minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
  • Application-compatible – effective in all kinds of protein detection systems, including Western blots (membranes), ELISA (microplates) and arrays (coated glass slides)
  • Streptavidin-friendly – absolutely free of biotin; no possible interference with avidin-biotin detection systems
  • High-performance – optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)
  • Convenient – supplied as ready-to-use 1X formulations in TBS and PBS with and without 0.05% Tween™ 20

Product Details:

Figure 2. Protein-Free Blocking Buffer efficiently blocks Western Blotting membranes.
Thermo Scientific Pierce Protein-Free Blocking Buffer efficiently blocks Western Blotting membranes. Jurkat Apoptotic Lysate (lane 1: 0.25µg, lane 2: 0.50µg) was separated in 4-20% Tris-glycine gels and transferred to nitrocellulose or PVDF membranes. The membranes were blocked for 1 hour at room temperature with the indicated blocking buffer, probed with mouse anti-PARP (0.25µg/mL) followed by goat anti-mouse HRP (4ng/mL) and detected by chemiluminescence with Thermo Scientific SuperSignal West Dura Substrate.

In most experimental situations, blocking buffers based on readily available animal proteins provide the best control and elimination of nonspecific and off-target binding interactions between antibody probes and assay platforms (nitrocellulose or PVDF membranes, polystyrene microplates or glass slides). However, certain sample-and-antibody combinations require the elimination of all possible exogenous animal proteins in the assay system to avoid cross-reaction or quenching of the desired probe function.

Various manufacturers have proposed the use of blocking buffers based on non-mammalian or non-animal proteins, suggesting that plant or bacterial proteins are less likely to cross-react with typical mammalian samples and antibodies. However, our experiments suggest that these non-animal protein blockers do not perform very well compared to Pierce Protein-Free Blocking Buffer, which is completely devoid of proteins (animal or otherwise).

Protein-Free Blocking Buffers exhibit less background than other blocking buffers in Multiplex SearchLight Arrays.
Thermo Scientific Pierce Protein-Free Blocking Buffers exhibit less background than other commercial alternatives to animal protein blocking agents. Multiplex protein arrays were created by spotting 12 cytokine capture antibodies per well of 96-well microplates. The ELISA plates were then blocked with the indicated blocking buffers, probed with HRP-conjugated secondary antibody (no samples) and the background for each well determined by chemiluminescence. Error bars represent the standard deviation for triplicate microplate wells.

Related Resources:

Blocking buffers for Western blot and ELISA -- technical guide
Blocking strategies for immunohistochemistry -- technical guide

Related Products:

All Blocking Buffers
Pierce ECL Western Blotting Substrate
Restore Western Blot Stripping Buffers
SuperSignal West Pico Chemiluminescent Substrate
SuperSignal ELISA Pico Chemiluminescent Substrate
1-Step Ultra TMB ELISA Substrate


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
37584 Protein-Free (PBS) Blocking Buffer
Formulation: Proprietary compound in phosphate-buffered saline, pH 7.4 with Kathon Antimicrobial Agent
Sufficient For: 3 blots or microplates at 30mL each
100mL Product Instructions for product #37584 Protein-Free (PBS) Blocking Buffer MSDS for product #37584 Protein-Free (PBS) Blocking Buffer Certificate of Analysis for product #37584 Protein-Free (PBS) Blocking Buffer $36.00

Add to cart
37572 Protein-Free (PBS) Blocking Buffer
Formulation: Proprietary compound in Phosphate-buffered saline, pH 7.4, with Kathon preservative
Sufficient For: 30 blots or microplates at 30mL each
1L Product Instructions for product #37572 Protein-Free (PBS) Blocking Buffer MSDS for product #37572 Protein-Free (PBS) Blocking Buffer Certificate of Analysis for product #37572 Protein-Free (PBS) Blocking Buffer $159.00

Add to cart
37573 Protein-Free T20 (PBS) Blocking Buffer
Formulation: Proprietary compound in Phosphate-buffered saline, pH 7.4, with 0.05% Tween-20 and Kathon preservative
Sufficient For: 30 blots or microplates at 30mL each
1L Product Instructions for product #37573 Protein-Free T20 (PBS) Blocking Buffer MSDS for product #37573 Protein-Free T20 (PBS) Blocking Buffer Certificate of Analysis for product #37573 Protein-Free T20 (PBS) Blocking Buffer $174.00

Add to cart
37585 Protein-Free (TBS) Blocking Buffer
Formulation: Proprietary compound in Tris-buffered saline, pH 7.4 with Kathon Antimicrobial Agent
Sufficient For: 3 blots or microplates at 30mL each
100mL Product Instructions for product #37585 Protein-Free (TBS) Blocking Buffer MSDS for product #37585 Protein-Free (TBS) Blocking Buffer Certificate of Analysis for product #37585 Protein-Free (TBS) Blocking Buffer $36.00

Add to cart
37570 Protein-Free (TBS) Blocking Buffer
Formulation: Proprietary compound in Tris-buffered saline, pH 7.4, with Kathon preservative
Sufficient For: 30 blots or microplates at 30mL each
1L Product Instructions for product #37570 Protein-Free (TBS) Blocking Buffer MSDS for product #37570 Protein-Free (TBS) Blocking Buffer Certificate of Analysis for product #37570 Protein-Free (TBS) Blocking Buffer $159.00

Add to cart
37571 Protein-Free T20 (TBS) Blocking Buffer
Formulation: Proprietary compound in Tris-buffered saline, pH 7.4, with 0.05% Tween-20 and Kathon preservative
Sufficient For: 30 blots or microplates at 30mL each
1L Product Instructions for product #37571 Protein-Free T20 (TBS) Blocking Buffer MSDS for product #37571 Protein-Free T20 (TBS) Blocking Buffer Certificate of Analysis for product #37571 Protein-Free T20 (TBS) Blocking Buffer $174.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

You may also need
23209 - Bovine Serum Albumin Standard Ampules, 2mg/mL

23227 - BCA Protein Assay Kit

66380 - Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 3mL

23225 - BCA Protein Assay Kit

66810 - Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 12mL

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747