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Pierce Protein Concentrators, 150K MWCO


Disposable ultrafiltration centrifugal devices for protein concentration.

Pierce Protein Concentrators, 150K MWCO

Thermo Scientific Pierce Protein Concentrators, 150K are disposable centrifugal devices for high-performance concentration, diafiltration or buffer-exchange of biological samples.

Pierce Protein Concentrators effectively combine simplicity, speed, capacity and recovery to yield enhanced concentration, purification and separation of proteins, even with dilute protein samples. The high-performance regenerated cellulose membranes in these 150K molecular-weight cutoff (MWCO) units are carefully and accurately rated to provide more than 90% protein recovery for proteins larger than 150,000 daltons. Pierce Concentrators are available in 7mL and 20mL sizes, which are compatible with swinging-bucket and fixed-angle rotors. These centrifugal concentrators provide efficient concentration of biological samples containing proteins (antigens, antibodies and enzymes), nucleic acids (DNA) and micro organisms (viral particles).

Highlights:

  • Rapid protein concentration – achieve greater than 100-fold protein concentration in less than 45 minutes
  • Excellent protein recovery – remove buffer and small molecules while retaining more than 90% of proteins greater than 150kDa
  • Convenient – two sizes designed to fit 15 and 50mL conical tubes; ideal for simple concentration of 1 to 20mL of sample
  • Instrument-compatible – use standard centrifuges with swinging-bucket or fixed-angle rotors; collect and recover sample without invert spinning
  • Versatile – the easiest way to perform sample concentration, diafiltration or buffer-exchange of biological samples

Requires:

Centrifuge equipped with a rotor for 15 or 50mL conical tubes (for 7 or 20mL Concentrators, respectively)

Applications:

  • Protein concentration with tissue culture media, antiserum or monoclonal antibody preps
  • Concentration of protein peaks following gel-permeation chromatography
  • Removal of unincorporated protein label
  • Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate or affinity chromatography columns

Product Details:

Pierce Protein Concentrators have a unique conical high-performance design that overcomes many problems associated with traditional protein concentration. These provide a high degree of concentration in a single centrifugation step while minimizing polarization and adsorption at the membrane surface. The regenerated cellulose membranes are accurately rated for molecular-weight cutoffs (MWCO) and routinely provide greater than 90% recovery of proteins larger than the membrane MWCO. Concentration factors greater than 100-fold can be obtained in less than 45 minutes. The Pierce Concentrators also allow accurate control of the dead-stop volume and final concentration factor, providing reliable and consistent sample processing, especially with low-concentration samples that are often lost or damaged with other centrifugal concentrator devices.

Small figures can be positioned with title and legend placed alongside.
Thermo Scientific Pierce Protein Concentrators make sample loading and recovery easy. Simply load up to 20mL of sample into the large concentration device (or up to 7mL with the smaller unit), cap the device and insert it into a fixed angle or swinging bucket rotor. Centrifuge the sample for the desired amount of time and rpm and then recover the concentrated sample. No inverted spin or membrane washing is required due to the unique conical design.

Related Resources:

Dialysis and desalting (Technical Guide)

Related Products:

Browse all Pierce Protein Concentrators
Dialysis Membrane and Devices
Desalting Columns
Pre-formulated Buffers


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
89920 Protein Concentrators, 150K MWCO, 7mL
Formulation: Cellulose membrane bonded to polypropylene tube
Sufficient For: 10 samples, each 1 to 7mL
10 concentrators Product Instructions for product #89920 Protein Concentrators, 150K MWCO, 7mL Certificate of Analysis for product #89920 Protein Concentrators, 150K MWCO, 7mL $68.00

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89922 Protein Concentrators, 150K MWCO, 7mL
Formulation: Cellulose membrane bonded to polypropylene tube
Sufficient For: 25 samples, each 1 to 7mL
25 concentrators Product Instructions for product #89922 Protein Concentrators, 150K MWCO, 7mL Certificate of Analysis for product #89922 Protein Concentrators, 150K MWCO, 7mL $158.00

Add to cart
89921 Protein Concentrators, 150K MWCO, 20mL
Formulation: Cellulose membrane bonded to polypropylene tube
Sufficient For: 10 samples, each 5 to 20mL
10 concentrators Product Instructions for product #89921 Protein Concentrators, 150K MWCO, 20mL Certificate of Analysis for product #89921 Protein Concentrators, 150K MWCO, 20mL $121.00

Add to cart
89923 Protein Concentrators, 150K MWCO, 20mL
Formulation: Cellulose membrane bonded to polypropylene tube
Sufficient For: 25 samples, each 5 to 20mL
25 concentrators Product Instructions for product #89923 Protein Concentrators, 150K MWCO, 20mL Certificate of Analysis for product #89923 Protein Concentrators, 150K MWCO, 20mL $282.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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