Update profile
    Change password
    Sign out

Pierce Protease and Phosphatase Inhibitor Tablets


Complete protection from proteases and phosphatases in one reliable tablet.

Pierce Protease and Phosphatase Inhibitor Tablets are broad-spectrum protease and phosphatase inhibitors

Thermo Scientific Pierce Protease and Phosphatase Inhibitor Tablets contain both protease inhibitors and phosphatase inhibitors to provide broad-spectrum complete protection from dephosphorylation and proteolytic degradation.

The Pierce Protease and Phosphatase Inhibitor Tablets contain aprotinin, bestatin, E-64 and leupeptin to provide protection from degradation by serine-proteases, cysteine-proteases, aspartic acid-proteases. To prevent the dephosphorylation activity of serine/threonine and tyrosine phosphatases, each tablet also contains sodium fluoride, sodium orthovanadate, sodium pyrophosphate and beta-glycerophosphate. The formulation is available with or without EDTA, a metalloproteinase inhibitor that may interfere with certain downstream applications. Pierce Protease and Phosphatase Inhibitor Tablets are stable at 4°C for up to 12 months, and each tablet is sufficient for 10mL of solution.

Highlights:

  • Complete – all-in-one tablet contains both protease and phosphatase inhibitors
  • Easy-to-use – the refrigerator-stable tablets are easily dissolved in buffers and lysis reagents
  • Two formulations – available with or without EDTA
  • Compatible – use with homebrew or commercially available cell lysis reagents and Thermo Scientific Protein Assays

Applications:

  • Protect intact, active cellular proteins from degradation and/or dephosphorylation by endogenous proteases and phosphatases.
  • Preserve protein integrity and phosphorylation upstream of phosphoproteomic analyses and phospho-specific immunoassays.

Product Details:

Pierce Protease and Phosphatase Inhibitor Tablets are the first complete inhibitor tablet. Adding one tablet to 10mL of lysis buffer provides complete protection from protease and phosphatase activity during protein extraction and protein purification. While both formulations have metalloprotease inhibitors, an EDTA-free formulation is available for applications that require divalent cations for protein activity. Similarly, EDTA and certain phosphatase inhibitors interfere with immobilized metal chelate affinity chromatography (IMAC) and 2D electrophoresis. Dialyze or desalt the sample before performing these procedures. The Protease and Phosphatase Inhibitor Tablets formulation is mass spec-compatible because it does not contain AEBSF.

Comparison of protease and phosphatase inhibitor activity.
Comparison of protease and phosphatase inhibitor activity. The degree of inhibition provided by Thermo Scientific Pierce Protease and Phosphatase Inhibitor Tablets was determined by incubating a quenched-fluorescent substrate with pancreatic tissue extract with or without inhibitors and measuring fluorescence after substrate cleavage. A. Protease assay reactions were incubated for 2 hours and 37°C. B. Acid phosphatase assay reactions were incubated for 1 hour at 37°C. The degree of inhibition is indicated for each inhibitor formulation.

 

More Product Data

Related Resources:

Review of protease and phosphatase inhibitors
Review of cell lysis and protein extraction

Related Products:

Pierce Protease Inhibitor Tablets
Pierce Phosphatase Inhibitor Tablets
Halt Inhibitor Cocktails
Individual Protease Inhibitors (AEBSF, aprotinin, leupeptin, PMSF, etc.)
Browse all Pierce Cell Lysis Solutions


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
88668 Pierce Protease and Phosphatase Inhibitor Mini Tablets
Formulation: Inhibitors in proprietary excipient in amber glass vials
Sufficient For: 200mL of sample (10mL each mini tablet)

Replaces Part No. 88663 (tablets in blister packs)
20 tablets Product Instructions for product #88668 Pierce Protease and Phosphatase Inhibitor Mini Tablets MSDS for product #88668 Pierce Protease and Phosphatase Inhibitor Mini Tablets Certificate of Analysis for product #88668 Pierce Protease and Phosphatase Inhibitor Mini Tablets $206.00

Add to cart
88669 Pierce Protease and Phosphatase Inhibitor Mini Tablets, EDTA Free
Formulation: Inhibitors in proprietary excipient amber glass vials
Sufficient For: 200mL of sample (10mL each mini tablet)

Replaces Part No. 88664 (tablets in blister packs)
20 tablets Product Instructions for product #88669 Pierce Protease and Phosphatase Inhibitor Mini Tablets, EDTA Free MSDS for product #88669 Pierce Protease and Phosphatase Inhibitor Mini Tablets, EDTA Free Certificate of Analysis for product #88669 Pierce Protease and Phosphatase Inhibitor Mini Tablets, EDTA Free $206.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Share This Page
 
 

Follow Us
  Email Sign-up  Email Announcements

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747