Thermo Scientific Pierce Protease Inhibitor Tablets and Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments.
Pierce Protease Inhibitor Tablets protect proteins from proteolytic degradation through inhibition of the serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. The tablets contain AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A and are available in formulations with or without EDTA, a metalloproteinase inhibitor. The EDTA-free formulation has added compatibility with IMAC purification methods and 2D gel electrophoresis. Stable for storage at 4°C for up to 12 months, each large tablet is sufficient for 50mL of solution and each mini tablet is sufficient for 10mL of solution. Both sizes and formulations of tablet are suitable for reagent preparation prior to protein extraction from tissue and cultured cells.
- Economical – both large and mini tablets are more cost effective than other commercially available formulations for equivalent volumes
- Easy-to-use – the refrigerator-stable tablets are easily dissolved in buffers and lysis reagents
- Two formulations – available with or without EDTA
- Compatible – use with homebrew or commercially available cell lysis reagents and Thermo Scientific Protein Assays
Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids. Proteases are regulated and compartmentalized in living cells. Protein extraction from tissues and cultured cells causes their release and ability to degrade other valuable proteins, adversely affecting downstream applications. For this reason, it is common practice to add multiple compounds known to inactivate or inhibit proteases to cell lysis buffers or cell extracts.
To use Pierce Protease Inhibitor Mini and large Tablets, simply dissolve 1 tablet in 10 or 50mL (respectively) of buffer or lysate. The protease inhibitor formulation is compatible with most detergent-based cell lysis reagents, including Thermo Scientific Pierce Cell Lysis Solutions. Pierce Protease Inhibitor Tablets do not contain phosphatase inhibitors. If necessary, phosphatase inhibitors, such as Thermo Scientific Pierce Phosphatase Inhibitor Tablets, can be added along with the protease inhibitor tablets.
|Comparison of commercially available protease inhibitor tablets and cocktails. Pancreatic extract (50µL; 1µg/µL protein) or trypsin (25µL, 0.1 units/µL) was incubated with a quenched-fluorescent protease-cleavable substrate for cysteine (A) or serine proteases (B) in the presence or absence of commercially available protease inhibitors with EDTA-containing (blue) or EDTA-free (purple) formulations. Reactions were incubated for 2 hours at 37°C and the fluorescence determined at indicated detecting emissions. The percent protease inhibition is indicated for each protease inhibitor formulation.
Review of Protease and Phosphatase Inhibitors
Review of Cell Lysis and Protein Extraction
Pierce Cell Lysis Handbook
Pierce Phosphatase Inhibitor Tablets
Pierce Protease and Phosphatase Inhibitor Tablets
Halt Inhibitor Cocktails
Individual Protease Inhibitors (AEBSF, aprotinin, leupeptin, PMSF, etc.)
Browse all Pierce Cell Lysis Solutions